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Comparative morphometry of precompacted bovine embryos produced in vivo or in vitro after parthenogenetic activation and intracytoplasmic sperm injection (ICSI): ultrastructural analysis

Published online by Cambridge University Press:  27 November 2003

J. Pivko
Affiliation:
Institute for Genetics and Reproduction, Research Institute of Animal Production, Nitra, Slovakia
V. Landa
Affiliation:
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
E. Kubovičová
Affiliation:
Institute for Genetics and Reproduction, Research Institute of Animal Production, Nitra, Slovakia
A. šupová
Affiliation:
Department of Zoology and Anthropology, Faculty of Science, Konstantin The Philosopher University, Nitra, Slovakia
P. Grafenau
Affiliation:
Institute for Genetics and Reproduction, Research Institute of Animal Production, Nitra, Slovakia
A. Makarevič
Affiliation:
Institute for Genetics and Reproduction, Research Institute of Animal Production, Nitra, Slovakia
L'. Riha
Affiliation:
Institute for Genetics and Reproduction, Research Institute of Animal Production, Nitra, Slovakia
M. Zibrin
Affiliation:
Department of Anatomy, Histology and Physiology, University of Veterinary Medicine, Košice, Slovakia

Abstract

Early bovine precompacted embryos (1 to 8 blastomeres) were analysed by electron microscopy. The volume density of cellular components was determined by morphometric analysis to quantify the ultrastructure of early bovine embryos produced either in vivo or in vitro both after fertilisation by intracytoplasmic sperm injection (ICSI) or from electrically stimulated oocytes (AC/DC). In normal embryos obtained in vivo (control), most of the cellular volume was occupied by cytoplasm (82.93%). The relative volume of lipids, vacuoles, mitochondria, Golgi apparatus and inclusion bodies was minimal. In the group of embryos after parthenogenetic activation (AC/DC) a relatively high proportion of the volume was occupied by vacuoles and lipids (18.68% vs 14.33%). Early ICSI-derived embryos contained the lowest relative volume of cytoplasm (58.33%) compared with the control embryos (in vivo) and parthenogenetically AC/DC-activated embryos and a higher volume was occupied by lipids (13.25%) and vacuoles (12.92%). It is concluded that in vitro produced embryos have a significantly altered ultrastructure, indicating extensive cellular damage.

Type
Research Article
Copyright
2003 Cambridge University Press

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