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Combined immunocytogenetic and molecular cytogenetic analysis of meiosis I oocytes from normal human females

Published online by Cambridge University Press:  15 January 2010

A.L. Barlow  and
M.A. Hultén
A.L. Barlow
Affiliation:
LSF Research Unit, Regional Genetics Services, Heartlands Hospital, Birmingham, UK
M.A. Hultén*
Affiliation:
LSF Research Unit, Regional Genetics Services, Heartlands Hospital, Birmingham, UK
*
M.A. Hultén, Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK. Telephone: +44 (01203) 528976. Fax: +44 (01203) 523701. e-mail: [email protected].
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Summary

The microspread oocytes of three fetuses, two of 16 weeks gestation and one of 15 weeks gestation, were labelled with a combination of anti-lateral element antiserum and a human centromere labelling auto-immune serum. The anti-lateral element serum was found to label both asynapsed axial elements and synapsed lateral elements strongly. Nuclei were found from leptotene to diplotene in all three fetuses. The use of the human auto-immune serum led to the observation of ‘staggered centromeres’ and ‘centromeric associations’ as well as tightly clustered centromeres in ‘stellar nuclei’. Nuclei displaying various aberrant features were detected. The use of antibody-labelled microspread oocytes as substrates for fluorescence in situ hybridisation (FISH) was found to be reliably successful only with repetitive (centromeric and telomeric) probes.

Keywords

Fetal ovariesHumanMeiosisOocytesSynaptonemal complex
Type
Research Article
Information
Zygote , Volume 6 , Issue 1 , February 1998 , pp. 27 - 38
Copyright
Copyright © Cambridge University Press 1998

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