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Collection and in vitro maturation of Mazama gouazoubira (brown brocket deer) oocytes obtained after ovarian stimulation

Published online by Cambridge University Press:  15 January 2021

Luciana Diniz Rola Open the ORCID record for Luciana Diniz Rola [Opens in a new window] ,
Eveline dos Santos Zanetti ,
Maite del Collado ,
Ellen de Fátima Carvalho Peroni  and
José Maurício Barbanti Duarte
Luciana Diniz Rola*
Affiliation:
NUPECCE - Núcleo de Pesquisa e Conservação de Cervídeos, Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brazil Departamento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba,Areia, PB, Brazil
Eveline dos Santos Zanetti
Affiliation:
NUPECCE - Núcleo de Pesquisa e Conservação de Cervídeos, Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brazil
Maite del Collado
Affiliation:
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil
Ellen de Fátima Carvalho Peroni
Affiliation:
NUPECCE - Núcleo de Pesquisa e Conservação de Cervídeos, Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brazil
José Maurício Barbanti Duarte
Affiliation:
NUPECCE - Núcleo de Pesquisa e Conservação de Cervídeos, Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brazil
*
Author for correspondence: Luciana D. Rola. Centro de Ciências Agrárias, Universidade Federal da Paraíba, Rodovia PB 079 - Km 12, Areia, PB, CEP: 58.397-000, Brazil. Tel: +55 83 3362-1700. E-mail: [email protected]
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Summary

In vitro production of embryos has gained prominence as a tool for use in wildlife conservation programmes in situ and ex situ. However, the development of this technique depends on steps that include ovarian stimulation, collection and oocyte maturation. The purpose of this study was to assess the feasibility of an ovarian stimulation protocol for follicular aspiration, the efficiency of videolaparoscopy for follicular aspiration and test a medium for in vitro oocyte maturation for the species Mazama gouazoubira. Five females were submitted to repeated ovarian stimulation (hormone protocol using controlled internal drug release), and estradiol benzoate on D0 and eight injections of follicle-stimulating hormone, once every 12 h, from D4 onwards at 30-day intervals. Fourteen surgical procedures were performed in superstimulated females, resulting in the collection of 94 oocytes and an average of 17.1 ± 9.1 follicles observed, 13.5 ± 6.6 follicles aspirated and 7.2 ± 3.7 oocytes collected per surgery. After collection, the oocytes were submitted to in vitro maturation for 24 h and stained with Hoechst 33342 dye to evaluate their nuclear status; 64.5% of the oocytes reached MII and 16.1% were spontaneously activated by parthenogenesis. The nuclear status of oocytes that did not undergo in vitro maturation was evaluated; 80.9% were found to be immature.

Keywords

Assisted reproductive technologiesDeerFSHLaparoscopic ovum pick-upWild animals
Type
Research Article
Information
Zygote , Volume 29 , Issue 3 , June 2021 , pp. 216 - 222
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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