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Coenzyme Q10 ameliorates the quality of mouse oocytes during in vitro culture

Published online by Cambridge University Press:  25 August 2021

Chan Hee Lee ,
Min Kook Kang ,
Dong Hyun Sohn ,
Hye Min Kim ,
Juri Yang  and
Seung Jin Han Open the ORCID record for Seung Jin Han [Opens in a new window]
Chan Hee Lee
Affiliation:
Department of Biological Science, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 50834, Republic of Korea
Min Kook Kang
Affiliation:
Department of Biological Science, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 50834, Republic of Korea Department of Research Center, Dong Nam Institute of Radiological and Medical Sciences, 46033, Republic of Korea
Dong Hyun Sohn
Affiliation:
Department of Microbiology and Immunology, Pusan National University School of Medicine, Yangsan, Gyeongnam 50612, Republic of Korea
Hye Min Kim
Affiliation:
Department of Biological Science, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 50834, Republic of Korea Institute of Digital Anti-Aging Healthcare, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 50834, Republic of Korea
Juri Yang
Affiliation:
Department of Biological Science, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 50834, Republic of Korea Institute of Digital Anti-Aging Healthcare, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 50834, Republic of Korea
Seung Jin Han*
Affiliation:
Department of Biological Science, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 50834, Republic of Korea Department of Biotechnology, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 50834, Republic of Korea Institute of Digital Anti-Aging Healthcare, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 50834, Republic of Korea Institute of Basic Science, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 50834, Republic of Korea
*
Autor for correspondence: Seung Jin Han. Department of Biotechnology, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 50834, Republic of Korea. Tel: +82 55 320 3787. Fax: +82 55 336 7706. E-mail: [email protected]
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Summary

Oxidative stress causes several diseases and dysfunctions in cells, including oocytes. Clearly, oxidative stress influences oocyte quality during in vitro maturation and fertilization. Here we tested the ability of coenzyme Q10 (CoQ10) to reduce reactive oxygen species (ROS) and improve mouse oocyte quality during in vitro culture. Treatment with 50 μM CoQ10 efficiently reduced ROS levels in oocytes cultured in vitro. The fertilizable form of an oocyte usually contains a cortical granule-free domain (CGFD). CoQ10 enhanced the ratio of CGFD–oocytes from 35% to 45%. However, the hardening of the zona pellucida in oocytes was not affected by CoQ10 treatment. The in vitro maturation capacity of oocytes, which was determined by the first polar body extrusion, was enhanced from 48.9% to 75.7% by the addition of CoQ10 to the culture medium. During the parthenogenesis process, the number of two-cell embryos was increased by CoQ10 from 43.5% to 67.3%. Additionally, treatment with CoQ10 increased the expression of Bcl2 and Sirt1 in cumulus cells. These results suggested that CoQ10 had a positive effect on ROS reduction, maturation rate and two-cell embryo formation in mouse oocyte culture.

Keywords

Anti-ageingCoenzyme Q10In vitro cultureOocyteReactive oxygen species
Type
Research Article
Information
Zygote , Volume 30 , Issue 2 , April 2022 , pp. 249 - 257
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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