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Chemical activation in Rhinella arenarum oocytes: effect of dehydroleucodine (DhL) and its hydrogenated derivative (2H-DhL)

Published online by Cambridge University Press:  26 November 2014

M.F. Medina ,
M.I. Bühler  and
G. Sánchez-Toranzo
M.F. Medina*
Affiliation:
Departamento de Biología del Desarrollo (INSIBIO), Chacabuco 461, 4000 San Miguel de Tucumán, Argentina.
M.I. Bühler
Affiliation:
Departamento de Biología del Desarrollo (INSIBIO); and Instituto de Biología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina.
G. Sánchez-Toranzo
Affiliation:
Departamento de Biología del Desarrollo (INSIBIO); and Instituto de Biología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina.
*
All correspondence to: Maria Fernanda Medina, Departamento de Biología del Desarrollo (INSIBIO), Chacabuco 461, 4000 San Miguel de Tucumán, Argentina. Fax: +54 381 4248025. e-mail: [email protected]
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Summary

Mature oocytes are arrested in metaphase II due to the presence of high levels of active maturation promoting factor (MPF). After fertilization, active MPF levels decline abruptly, enabling oocytes to complete meiosis II. One of the first and universal events of oocyte activation is an increase in cytosolic Ca2+ that would be responsible for MPF inactivation. Mature oocytes can also be activated by parthenogenetic activation. The aims of this work are to test the ability of dehydroleucodine (DhL) and its hydrogenated derivative 11,13-dihydro-dehydroleucodine (2H-DhL) to induce chemical activation in amphibian oocytes and to study the participation of calcium in the process. Results indicated that DhL and 2H-DhL induced oocyte activation in a dose-dependent manner. After 90 min of treatment, DhL 36 μM was able to induce 95% activation, while 2H-DhL 36 μM was less active, with only 40% activation. Our results suggest that DhL induced the inhibition of MPF activity, probably by an increase in intracellular Ca2+ concentration. Extracellular Ca2+ would not be significant, although Ca2+ release from intracellular stores is critical. In this sense, IP3Rs and RyRs were involved in the Ca2+ transient induced by lactones. In this species, RyRs appears to be the largest contributor to Ca2+ release in DhL-induced activation. Although more studies are needed on the mechanism of action through which these lactones induce oocyte activation in Rhinella arenarum, the results of this research provide interesting perspectives for the use of these lactones as chemical activators in in vitro fertilization and cloning.

Keywords

CalciumDehydroleucodineOocyte activationRhinella arenarum
Type
Research Article
Information
Zygote , Volume 23 , Issue 6 , December 2015 , pp. 924 - 932
Copyright
Copyright © Cambridge University Press 2014 

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References

Abrieu, A., Doree, M. & Fisher, D. (2001). The interplay between cyclin-B-Cdc2 kinase (MPF) and MAP kinase during maturation of oocytes. J. Cell Sci. 114, 257–67.Google Scholar
Ajmat, M.T., Bonilla, F., Zelarayán, L & Bühler, M.I. (2011). Participation of inositol trisphosphate and ryanodine receptors in Bufo arenarum oocyte activation. Zygote 19, 171–80.CrossRefGoogle ScholarPubMed
Ajmat, M.T., Bonilla, F., Hermosilla, P.C., Zelarayán, L. & Bühler, M.I. (2013). Role of phospholipase A2 pathway in regulating activation of Bufo arenarum oocytes. Zygote 21, 214–20.Google Scholar
Bhak, J.S., Lee, S.L., Ock, S.A., Mohana Kumar, B., Choe, S.Y. & Rho, G.J. (2006). Developmental rate and ploidy of embryos produced by nuclear transfer with different activation treatments in cattle. Anim. Reprod. Sci. 92 (1–2), 37–49.Google Scholar
Cruzado, M., Castro, C., Fernandez, D., Gomez, L., Roque, M., Giordano, O.E. & Lopez, L.A. (2005). Dehydroleucodine inhibits vascular smooth muscle cell proliferation in G2 phase. Cell. Mol. Biol. (Noisy-le-grand) 51, 525–30.Google Scholar
Deguchi, R., Osanai, K. & Morisawa, M. (1996). Extracellular Ca2+ entry and Ca2+ release from inositol 1,4,5-trisphosphate-sensitive stores function at fertilization in oocytes of the marine bivalve Mytilus edulis . Development 122, 3651–60.CrossRefGoogle ScholarPubMed
Galas, S., Barakat, , Doreé, H., Picard, M., , A. (1993). A nuclear factor required for specific translation of cyclin B may control the timing of first meiotic cleavage in starfish oocytes. Mol. Biol. Cell. 4, 1295–306.Google Scholar
Gautier, J. & Maller, J.L. (1991). Cyclin B in Xenopus oocytes: implications for the mechanism of pre-MPF activation. EMBO J. 10, 77–182.CrossRefGoogle ScholarPubMed
Giordano, O.S., Guerreiro, E., Pestchanker, M.J., Guzman, J., Pastor, D. & Guardia, T. (1990). The gastric cytoprotective effect of several sesquiterpene lactones. J. Nat. Prod. 53, 803–9.CrossRefGoogle ScholarPubMed
Giordano, O.S., Pestchanker, M.J., Guerreiro, E., Saad, J.R., Enriz, R.D., Rodriguez, A.M., Jauregui, E.A., Guzman, J., Maria, A.O. & Wendel, GH. (1992). Structure-activity relationship in the gastric cytoprotective effect of several sesquiterpene lactones. J. Med. Chem. 35, 2452–8.Google Scholar
Kline, D. & Kline, J.T. (1992). Thapsigargin activates a calcium influx pathway in the unfertilized mouse egg and suppresses repetitive calcium transients in the fertilized egg. J. Biol. Chem. 267, 17624–30.Google Scholar
Liu, C.T., Chen, C.H., Cheng, S.P. & Ju, J.C. (2002). Parthenogenesis of rabbit oocytes activated by different stimuli. Anim. Reprod. Sci. 70, 267–76.Google Scholar
López, M.E., Giordano, O.S. & López, L.A. (2002). Sesquiterpene lactone dehydroleucodine selectively induces transient arrest in G2 in Allium cepa root meristematic cells. Protoplasma 219 (1–2), 82–8.Google Scholar
Minshull, J., Murray, A., Colman, A. & Hunt, T. (1991). Xenopus oocyte maturation does not require new cyclin synthesis. J. Cell Biol. 114, 767–72.Google Scholar
Miyazaki, S., Shirakawa, H., Nakada, K. & Honda, Y. (1993). Essential role of the inositol 1,4,5-trisphosphate receptor/Ca2+ release channel in Ca2+ waves and Ca2+ oscillations at fertilization of mammalian eggs. Dev Biol. 158, 62–78.Google Scholar
Ookata, K., Hisanaga, S., Okano, T., Tachibana, K. & Kishimoto, T. (1992). Relocation and distinct subcellular localization of p34cdc2-cyclin B complex at meiosis reinitiation in starfish oocytes. EMBO J. 11, 1763–72.Google Scholar
Polo, L.M., Castro, C.M., Cruzado, MC., Collino, C.J.G., Cuello-Carrión, F.D., Ciocca, D.R., Giordano, O.S., Ferrari, M. & López, L.A. (2007). 11,13-Dihydro-dehydroleucodine, a derivative of dehydroleucodine with an inactivated alkylating function conserves the anti-proliferative activity in G2 but does not cause cytotoxicity. Eur. J. Pharmacol. 556, 19–26.CrossRefGoogle Scholar
Sánchez Toranzo, G., Giordano, O.S., López, L.A. & Bühler, M.I. (2007). Effect of dehydroleucodine on meiosis reinitiation in Bufo arenarum denuded oocytes. Zygote 15, 183–7.Google Scholar
Sánchez Toranzo, G., López, L.A., Martínez, J.Z., Bühler, M.C. & Bühler, M.I. (2009). Involvement of the dehydroleucodine alpha-methylene-gamma-lactone function in GVBD inhibition in Bufo arenarum oocytes. Zygote 18, 41–9.Google Scholar
Sánchez Toranzo, G., Bühler, M.C. & Bühler, M.I. (2012). Participation of IP3R, RyR and L-type Ca2+ channel in the nuclear maturation of Rhinella arenarum oocytes. Zygote 22, 110–23.Google Scholar
Siriwan, D., Naruse, T. & Tamura, H. (2011). Effect of epoxides and α -methylene-γ-lactone skeleton of sesquiterpenes from yacon (Smallanthus sonchifolius) leaves on caspase-dependent apoptosis and NF-κB inhibition in human cervical cancer cells. Fitoterapia 82, 1093–101.Google Scholar
Schultz, R.M. & Kopf, G.S. (1995). Molecular basis of mammalian egg activation. Curr. Top. Dev. Biol. 30, 21–62.Google Scholar
Wang, L., White, K.L., Reed, W.A. & Campbell, K.D. (2005). Dynamic changes to the inositol 1,4,5-trisphosphate and ryanodine receptors during maturation of bovine oocytes. Cloning Stem Cells 7, 306–20.Google Scholar
Watanabe, N., Hunt, T., Ikawa, Y. & Sagata, N. (1991). Independent inactivation of MPF and cytostatic factor (Mos) upon fertilization of Xenopus eggs. Nature 352, 247–248.Google Scholar
Zelarayán, LI., Toranzo, G.S., Oterino, J.M. & Bühler, M.I. (2004). The role of calcium in the nuclear maturation of Bufo arenarum oocytes. Zygote 12, 49–56.Google Scholar