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Characterization of isolated bovine preantral follicles based on morphology, diameter and cell number

Published online by Cambridge University Press:  14 January 2020

Juliana I. Candelaria  and
Anna C. Denicol Open the ORCID record for Anna C. Denicol [Opens in a new window]
Juliana I. Candelaria
Affiliation:
Department of Animal Science, University of California, Davis, CA, USA
Anna C. Denicol*
Affiliation:
Department of Animal Science, University of California, Davis, CA, USA
*
Author for correspondence: Anna C. Denicol. Department of Animal Science, University of California, 450 Bioletti Way, Davis, CA, 95616, USA. Tel: +1 352 665 1909. E-mail: [email protected]
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Summary

Preantral follicles are a potential reservoir of oocytes to be used in assisted reproductive technologies. With the increasing interest in developing techniques to grow preantral follicles in vitro, and as the bovine emerges as an appropriate model species to understand human folliculogenesis, the establishment of an accurate classification of developmental stages is needed. Classification of bovine preantral follicles has been mostly based on histological analysis and estimation models, which may not translate well to correctly characterize preantral follicles isolated from the ovary. In this study, we classified bovine preantral follicles by morphology upon isolation, determined diameter and number of granulosa cells by direct counting, and compared our results with previous studies reporting bovine preantral follicle classification. Follicles were isolated via homogenization of ovary tissue and classified into primary, early secondary and secondary stage based on morphology and number of layers of granulosa cells. Diameter was individually measured and Hoechst 33342 was used as a nuclear stain to count granulosa cells. We found that follicles classified by morphology into primary, early secondary, and secondary had different mean diameter and cell number (P < 0.01); cell number and diameter were positively correlated, as were cell density and cell number in each developmental stage (P < 0.01). Results obtained here were mostly in agreement with previous classifications based on histological sections and on isolated follicles, with some discrepancies. The present data add accuracy to classification of bovine preantral follicles that is critical to optimize culture conditions to produce developmentally competent oocytes.

Keywords

BovineClassificationFollicle morphologyFolliculogenesisPreantral follicle
Type
Research Article
Information
Zygote , Volume 28 , Issue 2 , April 2020 , pp. 154 - 159
Copyright
© Cambridge University Press 2020

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