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Centrosomes with striated rootlets in rabbit zygotes

Published online by Cambridge University Press:  26 September 2008

M.S. Szöllösi ,
P. Adenot  and
D. Szöllösi
M.S. Szöllösi*
Affiliation:
Insitut de Biologie Physico-Chimique, Paries, and INRA, Jouy-en-Josas, France.
P. Adenot
Affiliation:
Insitut de Biologie Physico-Chimique, Paries, and INRA, Jouy-en-Josas, France.
D. Szöllösi
Affiliation:
Insitut de Biologie Physico-Chimique, Paries, and INRA, Jouy-en-Josas, France.
*
Maria S. Szöllösi, Laboratorire Associé INSERM/INRA, IBPC, 13, rue P.et M.curie, 75006 Paris, France. Fax: +33 1 43 29 80 88. e-mail: [email protected].
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Summary

An electron microscopic study of the rabbit zygote has shown the presence of numerous paracrystalline structures (PSs) around the pronuclei. The majority of these structures are situated in the narrow space between pronuclei. The PSs during interphase are associated with small dense knobs, and filamentous material;some of them, namely those situated in the internuclear space, are also associated with striated rootlets. The PS and its appendages form a complex which nucleates microtubules during interphase and phase M. The structure of these complexes changes with the cell cycle. Striated rootlets disappear at G2/M. Dense Knobs and filamentous material separate from the PS, become loose and associate with numerous microtubules at the poles of the first mitotic spindle. PSs and their associated structures are considered to be a newly discovered morphological form of the centrosome.

Keywords

CentrosomeRabbit zygoteStriated rootlet
Type
Article
Information
Zygote , Volume 4 , Issue 3 , August 1996 , pp. 173 - 179
Copyright
Copyright © Cambridge University Press 1996

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References

Alieva, I.B. & Vorobjev, I.A. (1989). The morphofunctional characteristics of striated rootlets within centrosomes of the cultured PK-cells Tsitologija 31, 1016–19.Google Scholar
Alieva, I.B., Nadezhdina, E.S., Vaisberg, E.A. & Vorobjev, I.A. (1992). Microtubule and intermediate filament patterns around the centrosome in interphase cells. In: The Centrosome, ed. Kalnins, V.J., pp. 103–29. New York: Academic Press.CrossRefGoogle ScholarPubMed
Anderson, E. (1972). The localization of acid phosphatase and the uptake of horseradish peroxidase in the oocyte and follicle cells of mammals. In Oogenesis ed. Biggers, J.D. & Schuetz, A.W., pp. 87–117. Baltimore/London: University Park Press/Butterwoth.Google Scholar
Baron, A.T. & Salisbury, J.L. (1988). Identification and localization of a novel, cytoskeletal, centrosome-associated Protein in PtK2 cells. J. Cell Biol. 107, 2669–78.CrossRefGoogle ScholarPubMed
Bernardini, G., Stipani, R. & Melone, G. (1986). The ultrastructure of Xenopusspermatozoon. J. Ultrastruct. Mol Struct. Res. 94, 188&94.Google Scholar
Calarco, P.G., Donahue, R.P. & Szöllösi, D. (1972). Germinal vesicle breakdown in the mouse oocyte. J. Cell Sci. 10, 369–85.Google Scholar
Calarco-Gillam, P.D., Siebert, M.C., Hubble, R., Mitchison, T. & Kirschner, M. (1983). Centrosome development in early mouse embryos as defined by an autoantibody against pericentriolar material. Cell. 35, 621–9.CrossRefGoogle ScholarPubMed
Dales, S., Hsu, K.C. & Nagayama, A. (1973). The fine structure and immunological labeling of the achromatic mitotic apparatus after disruption of cell membranes. J. Cell Biol. 59, 643–60.CrossRefGoogle ScholarPubMed
Felix, M.-A., Antony, C., Wright, M. & Maro, B. (1994). Centrosome assembly in vitro: role of γ-tubulin recruitment in Xenopus sperm aster formation. J. Cell Biol. 124, 19–31.Google Scholar
Gondos, B. & Bhiraleus, P. (1970). Pronuclear relationship and association of maternal and paternal chromosomes in flushed rabbit ova. Z. Zellforsch. 111, 149–59.CrossRefGoogle ScholarPubMed
Hard, R. & Rieder, C.L. (1983). Muciliary transport in newt lungs: the ultrastucture of the ciliary apparatus in isolated epithelial sheets and in functional triton-extracted models. Tissue Cell. 15, 227–47.CrossRefGoogle Scholar
Kimble, M. & Kuriyama, R. (1992). Functional components of microtubule-organizing centers. Int. Rev. Cytol. 136, 1–50.Google Scholar
Lauwerys, J.M. & Boussauw, L. (1973). Striated filamentous bundles associated with centrioles in pulmonary lymphatic endothelial cells. J. Ultrastruct. Res. 42, 25–8.CrossRefGoogle Scholar
Longo, F.J. (1976). Sperm aster in rabbit zygotes: its structure and function. J. Cell Biol. 69, 539–47.Google Scholar
Nagano, T. (1962). An electron microscopic observation on the cross-striated fibrils occurring in the human spermatocyte. J. Zellforsch. 58, 214–18.CrossRefGoogle ScholarPubMed
Sakaguchi, H. (1965). Pericentriolar filamentous bodies. J. Ultrastruct. Res. 12, 13–21.CrossRefGoogle ScholarPubMed
Salisbury, J.L., Baron, A., Surek, B. & Melkonian, M. (1984). Striated flagellar roots: isolation and partial characterization of a calcium-modulated contractile organelle. J. Cell biol. 99, 962–70.Google Scholar
Schatten, G. (1994). The centrosome and its mode of inheritance: the reduction of the centrosome during gametogenesis and its restoration during fertilization. Dev. Biol. 165, 299–335.CrossRefGoogle ScholarPubMed
Szöllösi, D. & Ozil, J.-P. (1991). De novo formation of centrioles in parthenogetically activated, diploidized rabbit embryos. Biol. Cell. 72, 61–6.CrossRefGoogle Scholar
Vorobjev, I.A. & Chentsov, Y.S. (1977). The ultrastucture of the centrosome in cell of haemopoietic tissue of the axoloti. Tsitologijia. 19, 598–603.Google Scholar
Vorobjev, I.A. & Chentsov, Y.S. (1982). Centrioles in the cell cycle. Epithelial cells J.cell Biol. 98, 938–49.Google Scholar
Vorobjev, I.A. & Nadezhdina, E.S. (1987). The centrosome and its role in the organization of microtubules. Int. Rev Cytol. 106, 227–93.Google Scholar
Yllera Fernandez, M.M., Crozet, N. & Ahmed-Ali, M.. (1992). Microtubule distribution during fertilization in the rabbit. Mol. Reprod. Dev. 32, 271–6.CrossRefGoogle ScholarPubMed
Zamboni, L. & Mastroianni, L. Jr (1966). Electron microscopic studies on rabbit ova. I. The follicular oocyte. J. Ultrastruct. Res. 14, 95–117.CrossRefGoogle Scholar