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Biphasic assembly of the contractile apparatus during the first two cell division cycles in zebrafish embryos

Published online by Cambridge University Press:  27 March 2013

Sarah E. Webb ,
Cécile Goulet ,
Ching Man Chan ,
Michael Y. F. Yuen  and
Andrew L. Miller
Sarah E. Webb
Affiliation:
Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
Cécile Goulet
Affiliation:
Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
Ching Man Chan
Affiliation:
Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
Michael Y. F. Yuen
Affiliation:
Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
Andrew L. Miller*
Affiliation:
Division of Life Science, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China. Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA.
*
All correspondence to: Andrew L. Miller. Division of Life Science, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China. Tel: +1 852 2358 8631. Fax: +1 852 2358 1559. e-mail: [email protected]
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Summary

The large and optically clear embryos of the zebrafish provide an excellent model system in which to study the dynamic assembly of the essential contractile band components, actin and myosin, via double fluorescent labelling in combination with confocal microscopy. We report the rapid appearance (i.e. within <2 min) of a restricted arc of F-actin patches along the prospective furrow plane in a central, apical region of the blastodisc cortex. These patches then fused with each other end-to-end forming multiple actin cables, which were subsequently bundled together forming an F-actin band. During this initial assembly phase, the F-actin-based structure did not elongate laterally, but was still restricted to an arc extending ~15° either side of the blastodisc apex. This initial assembly phase was then followed by an extension phase, where additional F-actin patches were added to each end of the original arc, thus extending it out to the edges of the blastodisc. The dynamics of phosphorylated myosin light chain 2 (MLC2) recruitment to this F-actin scaffold also reflect the two-phase nature of the contractile apparatus assembly. MLC2 was not associated with the initial F-actin arc, but MLC2 clusters were recruited and assembled into the extending ends of the band. We propose that the MLC2-free central region of the contractile apparatus acts to position and then extend the cleavage furrow in the correct plane, while the actomyosin ends alone generate the force required for furrow ingression. This biphasic assembly strategy may be required to successfully divide the early cells of large embryos.

Keywords

ActinBiphasicMeroblastic cell divisionMyosin light chain 2Zebrafish
Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 218 - 228
Copyright
Copyright © Cambridge University Press 2013 

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