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Antioxidant and developmental capacity of retinol on the in vitro culture of rabbit embryos

Published online by Cambridge University Press:  05 October 2018

Ahmed M. Elomda ,
Mohamed F. Saad ,
Ayman M. Saeed ,
Ashraf Elsayed ,
Ahmed O. Abass ,
Hosam M. Safaa  and
Gamal M. K. Mehaisen Open the ORCID record for Gamal M. K. Mehaisen [Opens in a new window]
Ahmed M. Elomda
Affiliation:
Department of Animal Biotechnology, Animal Production Research Institute, Dokki, Giza, Egypt
Mohamed F. Saad
Affiliation:
Department of Animal Biotechnology, Animal Production Research Institute, Dokki, Giza, Egypt
Ayman M. Saeed
Affiliation:
Department of Animal Biotechnology, Animal Production Research Institute, Dokki, Giza, Egypt
Ashraf Elsayed
Affiliation:
Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt
Ahmed O. Abass
Affiliation:
Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt
Hosam M. Safaa
Affiliation:
Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt Bisha University, Bisha, Saudi Arabia
Gamal M. K. Mehaisen*
Affiliation:
Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt
*
Author for correspondence: Gamal M.K. Mehaisen. Department of Animal Production, Faculty of Agriculture, Cairo University, 7 Gamaa Street, 12613 Giza, Egypt. Tel: +201226797270. Fax: +20235717355. E-mail: [email protected]
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Summary

Oxidative stress is a major cause of defective embryo development during in vitro culture. Retinoids are recognized as non-enzymatic antioxidants and may have an important role in the regulation of cell differentiation and vertebrate development. However, there are not enough reports discussing the antioxidant and developmental capacity of retinoids, including retinol (RT), on the in vitro development of embryos recovered from livestock animals, particularly in rabbit species. Therefore, morula embryos obtained from nulliparous Red Baladi rabbit does were cultured for 48 h in TCM199 medium in the absence of RT (control group) or in the presence of RT at concentrations of 10, 100 and 1000 nM. The developmental capacity to the hatched blastocyst stage, the antioxidant biomarker assay and the expression of several selected genes were analyzed in each RT group. The data show that RT significantly (P<0.001) promoted the embryo hatchability rate at the concentration of 1000 nM to 69.44% versus 29.71% for the control. The activity of malondialdehyde (MDA) level was significantly (P<0.05) lower in the RT groups than in the control group, while the total antioxidant capacity (TAC), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were significantly (P<0.05) higher following treatment with RT. Furthermore, RT treatment considerably upregulated the relative expression of gap junction protein alpha 1 (GJA1), POU class 5 homeobox 1 (POU5F1) and superoxide dismutase 1 (SOD1) genes compared with the control group. The current study highlights the potential effects of RT as antioxidant in the culture medium on the in vitro development of rabbit embryos.

Keywords

Antioxidant biomarkersGene expressionIn vitro developmentRabbit embryoRetinol
Type
Research Article
Information
Zygote , Volume 26 , Issue 4 , August 2018 , pp. 326 - 332
Copyright
© Cambridge University Press 2018 

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