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Anethole improves blastocysts rates together with antioxidant capacity when added during bovine embryo culture rather than in the in vitro maturation medium

Published online by Cambridge University Press:  27 August 2019

J.C. Anjos ,
F.L.N. Aguiar Open the ORCID record for F.L.N. Aguiar [Opens in a new window] ,
N.A.R. Sá ,
J.F. Souza ,
F.W.S. Cibin ,
B.G. Alves ,
R.R. Santos Open the ORCID record for R.R. Santos [Opens in a new window]  and
J.R. Figueiredo
J.C. Anjos
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil
F.L.N. Aguiar
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil
N.A.R. Sá
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil
J.F. Souza
Affiliation:
Laboratory BryoEmbryo – Genetics and biotechnology, Araguaína, Tocantins, Brazil
F.W.S. Cibin
Affiliation:
Federal University of Pampa, Uruguaiana, Rio Grande do Sul, Brazil
B.G. Alves
Affiliation:
Federal University of Goiás, Postgraduate Programme in Animal Bioscience, Jataí, Goiás, Brazil
R.R. Santos
Affiliation:
Federal University of Pará, Castanhal, PA, Brazil
J.R. Figueiredo*
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil
*
Address for correspondence: José Ricardo de Figueiredo. Postgraduate Programme in Veterinary Science, State University of Ceará, Fortaleza, Ceará, Brazil. 1700 Paranjana Avenue, Itaperi Campus. Fortaleza, Ceará 60714-903, Brazil. Tel: +55 85 3101 9852. Fax: +55 85 3101 9840. E-mail: [email protected]
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Summary

We performed the exposure of bovine oocytes to anethole during in vitro maturation (0 or 300 µg/ml), during in vitro embryo production (0, 30, 300 or 2000 µg/ml), or during both periods to determine the rates of 2−4 cells embryos, blastocysts rates and cells numbers, as well as the production of reactive oxygen species (ROS). Bovine ovaries (n = 240) were collected from a local abattoir after slaughter and cumulus–oocyte complexes (COCs) with homogeneous and non-dark cytoplasm, surrounded by two or more compact layers of cumulus cells, and an intact zona pellucida were selected for in vitro maturatuion (IVM). Mature oocytes were then submitted to in vitro fertilization (IVF) and in vitro embryo production (IVP) in culture medium supplemented or not with different concentrations of anethole, as described above. Although IVM medium supplementation with 300 µg/ml anethole improved the rates of bovine blastocysts formation, we demonstrated that IVP medium supplementation with 30 µg/ml anethole, regardless of IVM medium enrichment, considerably enhanced blastocysts rates. Furthermore, ROS levels were decreased only when anethole was added to the IVP medium without previous IVM medium supplementation.

Keywords

AnetholeAntioxidantsIn vitro embryo productionOxidative stress
Type
Research Article
Information
Zygote , Volume 27 , Issue 6 , December 2019 , pp. 382 - 385
Copyright
© Cambridge University Press 2019 

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References

Cadenas, J, Maside, C, Ferreira, ACA, Vieira, LA, Leiva-Revilla, J, Paes, VM, Alves, BG, Brando, FZ, Rodriques, APR, Wheeler, MB and Figueiredo, JR (2017) Relationship between follicular dynamics and oocyte maturation during in vitro culture as a non-invasive sign of caprine oocyte meiotic competence. Theriogenology 107, 95–103.10.1016/j.theriogenology.2017.10.038CrossRefGoogle ScholarPubMed
Chowdhury, MMR, Choi, BH, Khan, I, Lee, KL, Mesalam, A, Song, SH, Xu, L, Joo, MD, Afrin, F and Kong, IK (2017) Supplementation of lycopene in maturation media improves embryo quality in vitro . Theriogenology 103, 173–84.CrossRefGoogle ScholarPubMed
Combelles, CM, Gupta, S and Agarwal, A (2009) Could oxidative stress influence the in vitro maturation of oocytes? Reprod Biomed Online 18, 864–80.CrossRefGoogle ScholarPubMed
Galicka, A, Kretowski, R, Nazaruk, J and Chechowska-Pasko, M (2014) Anethole prevents hydrogen peroxide-induced apoptosis and collagen metabolism alterations in human skin fibroblasts. Mol Cell Biochem 394, 217–24.CrossRefGoogle ScholarPubMed
Giustarini, D, Fanti, P, Sparatore, A, Matteucci, E and Rossi, R (2014) Anethole dithiolethione lowers the homocysteine and raises the glutathione levels in solid tissues and plasma of rats: a novel non-vitamin homocysteine-lowering agent. Biochem Pharmacol 89, 246–54.10.1016/j.bcp.2014.03.005CrossRefGoogle ScholarPubMed
Hidaka, T, Fukumoto, Y, Yamamoto, S, Ogata, Y and Horiuchi, T (2018) Variations in bovine embryo production between individual donors for OPU-IVF are closely related to glutathione concentrations in oocytes during in vitro maturation. Theriogenology 113, 176–82.10.1016/j.theriogenology.2018.03.002CrossRefGoogle ScholarPubMed
Khazaei, M and Aghaz, F (2017) Reactive oxygen species generation and use of antioxidants during in vitro maturation of oocytes. Int J Fertil Steril 11, 63–70.Google ScholarPubMed
Korkina, LG (2007) Phenylpropanoids as naturally occurring antioxidants: from plant defense to human health. Cell Mol Biol 53, 15–25.Google ScholarPubMed
Liang, R, Yu, WD, Du, JB, Yang, LJ, Yang, JJ, Xu, J, Shang, M and Guo, JZ (2007) Cystathionine beta synthase participates in murine oocyte maturation mediated by homocysteine. Reprod Toxicol 24, 89–96.CrossRefGoogle ScholarPubMed
Loetchutinat, S, Kothan, S, Dechsupa, J, Meesungnoen, J and Jay-Gerin, SM (2005) Spectrofluorometric determination of intracellular levels of reactive oxygen species in drug-sensitive and drug-resistant cancer cells using the 2′,7′-dichlorofluorescein diacetate assay. Radiat Phys Chem 72, 323–31.CrossRefGoogle Scholar
Marinovi, V and Valcheva-Kuzmanova, S (2015) Review on the pharmacological activities of anethole. Scripta Sci Pharm 2, 14–19.Google Scholar
Moore, SG and Hasler, JF (2017) A 100-year review: reproductive technologies in dairy science. J Dairy Sci 100, 10314–31.CrossRefGoogle ScholarPubMed
Papa, P, Papa, F, Oliveira, L, Guasti, P, Castilho, C and Giometti, IC (2015) Different extenders in the cryopreservation of bovine epididymal spermatozoa. Anim Reprod Sci 161, 58–63.10.1016/j.anireprosci.2015.08.004CrossRefGoogle ScholarPubMed
Roginsky, V (2003) Chain-breaking antioxidant activity of natural polyphenols as determined during the chain oxidation of methyl linoleate in Triton X-100 micelles. Arch Biochem Biophys 414, 261–70.10.1016/S0003-9861(03)00143-7CrossRefGoogle ScholarPubMed
Sá, NAR, Araújo, VRH, Correia, HV, Ferreira, ACA, Guerreiro, DD, Sampaio, AM, Escobar, E, Santos, FW, Moura, AA, Lobo, CH, Ceccatto, VM, Campello, CC, Rodriques, APR, Leal-Cardoso, JH and Figueiredo, JR (2017) Anethole improves the in vitro development of isolated caprine secondary follicles. Theriogenology 89, 226–34.CrossRefGoogle ScholarPubMed
Sá, NAR, Vieira, LA, Ferreira, ACA, Cadenas, J, Bruno, JB, Maside, C, Sousa, FQC, Cibin, FWS, Alves, BG, Rodriques, APR, Leal-Cardoso, JH, Gastal, EL and Fiqueiredo, JR (2019) Anethole supplementation during oocyte maturation improves in vitro production of bovine embryos. Reprod Sci [Epub ahead of print]CrossRefGoogle Scholar
Sapanidou, V, Taitzoglou, I, Tsakmakidis, I, Kourzelis, I, Fletouris, D, Theodoridis, A, Lavrentiadou, S and Tsantarliotou, M (2016) Protective effect of crocetin on bovine spermatozoa against oxidative stress during in vitro fertilization. Andrology 4, 1138–49.10.1111/andr.12248CrossRefGoogle ScholarPubMed
Simões, R, Feitosa, WB, Siqueira, AF, Nichi, M, Paula-Lopes, FF, Marques, MG, Peres, MA, Barnabe, VH, Visintin, JA and Assumpcao, ME (2013) Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome. Reproduction 146, 433–41.CrossRefGoogle ScholarPubMed
Souza, JF, Oliveira, CM, Lienou, LL, Cavalcante, TV, Alexandrino, E, Santos, RR, Rodrigues, APR, Campello, CC, Figueiredo, JR and Dias, FEF (2018a) Vitrification of bovine embryos followed by in vitro hatching and expansion. Zygote 26, 99–103.CrossRefGoogle Scholar
Souza, JF, Lienou, LL, Rodrigues, APR, Alexandrino, E, Cavalcante, TV, Santos, RR, Figueiredo, JR and Dias, FEF (2018b) Cryosurvival after exposure of IVF-derived Nellore embryos to different cryoprotectants and exposure times during vitrification. Cryobiology 84, 95–7.CrossRefGoogle ScholarPubMed
Sovernigo, TC, Adona, PR, Monzani, PS, Guemra, S, Barros, F, Lopes, FG and Leal, C (2017) Effects of supplementation of medium with different antioxidants during in vitro maturation of bovine oocytes on subsequent embryo production. Reprod Domest Anim 52, 561–69.CrossRefGoogle ScholarPubMed
Tavallali, V, Rahmati, S and Rowshan, V (2017) Characterization and influence of green synthesis of nano-sized zinc complex with 5-aminolevulinic acid on bioactive compounds of aniseed. Chem Biodivers [Epub ahead of print]CrossRefGoogle Scholar
Torres, V, Hamdi, M, Maillo, V, Urrego, R, Echeverri, JJ, López-Herrera, A, Gutierrez-Adan, A, Rizos, D and Sanchez-Calabuig, MJ (2019) Ascorbic acid-cyclodextrin complex alters the expression of genes associated with lipid metabolism in bovine in vitro produced embryos. Reprod Domest Anim 54, 55–62.CrossRefGoogle ScholarPubMed
Torres, V, Hamdi, M, Millán de la Blanca, MG, Urrego, ER, Echeverri, J, López-Herrera, A, Rizos, D, Gutierrez-Adan, A and Sanchez-Calabuig, MJ (2018) Resveratrol–cyclodextrin complex affects the expression. of genes associated with lipid metabolism in bovine in vitro produced embryos. Reprod Domest Anim 53, 850–8.10.1111/rda.13175CrossRefGoogle ScholarPubMed
Wrenzycki, C (2018) Gene expression analysis and in vitro production procedures for bovine preimplantation embryos: past highlights, present concepts and future prospects. Reprod Domest Anim 53, 14–9.CrossRefGoogle ScholarPubMed
Yu, S, Long, H, Lyu, Q, Zhang, Q, Yan, Z, Liang, HX, Chai, WR, Yan, Z, Kuang, YP and Qi, C (2014) Protective effect of quercetin on the development of preimplantation mouse embryos against hydrogen peroxide-induced oxidative injury. PLoS One 9, e89520.CrossRefGoogle ScholarPubMed