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Anethole improves blastocysts rates together with antioxidant capacity when added during bovine embryo culture rather than in the in vitro maturation medium

Published online by Cambridge University Press:  27 August 2019

J.C. Anjos ,
F.L.N. Aguiar Open the ORCID record for F.L.N. Aguiar [Opens in a new window] ,
N.A.R. Sá ,
J.F. Souza ,
F.W.S. Cibin ,
B.G. Alves ,
R.R. Santos Open the ORCID record for R.R. Santos [Opens in a new window]  and
J.R. Figueiredo
J.C. Anjos
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil
F.L.N. Aguiar
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil
N.A.R. Sá
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil
J.F. Souza
Affiliation:
Laboratory BryoEmbryo – Genetics and biotechnology, Araguaína, Tocantins, Brazil
F.W.S. Cibin
Affiliation:
Federal University of Pampa, Uruguaiana, Rio Grande do Sul, Brazil
B.G. Alves
Affiliation:
Federal University of Goiás, Postgraduate Programme in Animal Bioscience, Jataí, Goiás, Brazil
R.R. Santos
Affiliation:
Federal University of Pará, Castanhal, PA, Brazil
J.R. Figueiredo*
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil
*
Address for correspondence: José Ricardo de Figueiredo. Postgraduate Programme in Veterinary Science, State University of Ceará, Fortaleza, Ceará, Brazil. 1700 Paranjana Avenue, Itaperi Campus. Fortaleza, Ceará 60714-903, Brazil. Tel: +55 85 3101 9852. Fax: +55 85 3101 9840. E-mail: [email protected]
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Summary

We performed the exposure of bovine oocytes to anethole during in vitro maturation (0 or 300 µg/ml), during in vitro embryo production (0, 30, 300 or 2000 µg/ml), or during both periods to determine the rates of 2−4 cells embryos, blastocysts rates and cells numbers, as well as the production of reactive oxygen species (ROS). Bovine ovaries (n = 240) were collected from a local abattoir after slaughter and cumulus–oocyte complexes (COCs) with homogeneous and non-dark cytoplasm, surrounded by two or more compact layers of cumulus cells, and an intact zona pellucida were selected for in vitro maturatuion (IVM). Mature oocytes were then submitted to in vitro fertilization (IVF) and in vitro embryo production (IVP) in culture medium supplemented or not with different concentrations of anethole, as described above. Although IVM medium supplementation with 300 µg/ml anethole improved the rates of bovine blastocysts formation, we demonstrated that IVP medium supplementation with 30 µg/ml anethole, regardless of IVM medium enrichment, considerably enhanced blastocysts rates. Furthermore, ROS levels were decreased only when anethole was added to the IVP medium without previous IVM medium supplementation.

Keywords

AnetholeAntioxidantsIn vitro embryo productionOxidative stress
Type
Research Article
Information
Zygote , Volume 27 , Issue 6 , December 2019 , pp. 382 - 385
Copyright
© Cambridge University Press 2019 

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