Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-23T05:12:50.776Z Has data issue: false hasContentIssue false
  • English
  • Français

Activation of bovine oocytes penetrated after germinal vesicle breakdown

Published online by Cambridge University Press:  26 September 2008

Lalantha R. Abeydeera ,
Kiyoshi Okuda  and
Koji Niwa
Lalantha R. Abeydeera
Affiliation:
Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama, Japan
Kiyoshi Okuda
Affiliation:
Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama, Japan
Koji Niwa*
Affiliation:
Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama, Japan
*
K. Niwa, Division of Animal science and Technology, Faculty of Agriculture, Okayama University, Okayama 700, Japan. Telephone: 086-251-8328. Fax: 086-254-0714.
Get access Rights & Permissions [Opens in a new window]

Summary

The present study was designed to examine the ability of bovine oocytes, after germinal vesicle breakdown (GVBD), to be activated by sperm penetration and the sequence of sperm nuclear transformation. Bovine oovytes cultured for 8 h in maturation medium (tissue culture medium TCM-199 containing 10% fetal calf serum) were inseminated in Brackett and Oilphant's medium supplemented with bovine serum albumin (10 mg/ml), caffeine (5mM) and heparin (10 μg/ml). When oocytes were transferrred to the maturation medium 8 h after insemination and additionally cultured for 5−40 h at 39°C in 5% CO2 in air, 71−76% of oocytes were penetrated and polyspermy (67–75%) was common. The proportions of penetrated oocytes that were activated significantly increased with the lapse of the additional culture time, reaching 88% and 87% by 25 and 40 h after additional culture, respectively. When compared with unpenetrated oocytes, signifcantly higher proportions of penetrated oocytes, reached metaphase II or beyond 15 and 25 h after additional culture. After penetration, sperm nuclei were transformed into metaphase chromosomes and then to telophase chromomes before the formation of male pronuclei. These results provide evidence that bovine oocytes acquire the ability to respond to sperm-mediated activation soon after GVBD.

Keywords

BovineGerminal vesicle breakdownOocyte activationOocytesSperm penetration
Type
Article
Information
Zygote , Volume 2 , Issue 4 , November 1994 , pp. 273 - 279
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abeydeera, L.R. & Niwa, K.. (1992). Ability of in vitro maturing bovine oocytes to transform sperm nuclei to metaphase chromosomes. J. Reprod. Fertil. 96, 565–73.CrossRefGoogle ScholarPubMed
Abeydeera, L.R.. Niwa, K. & Okuda, K.. (1993). Maturation promoting factor (MPF) is responsible for the transformation of spern nulei to metaphase chromosomes in maturing boving oocytes in vitro. J. Reprod. Fertil. 98, 409–14.CrossRefGoogle Scholar
Balakier, H. & Czolowska, N.. (1977). Cytoplasmic control of nuclear maturation in mouse oocytes. Exp. Cell res. 110, 446–9.Google ScholarPubMed
Brackett, B.G. & Oliphant, G.. (1975). Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. 12, 260–74.CrossRefGoogle ScholarPubMed
Chian, R.C., Niwa, K. & Nakahara, H.. (1992). Effect of sperm penetration in vitro on completion of first meiosis by bovine oocytes arrested at various stages in culture. J. Reprod. Fertil. 96, 73–8.CrossRefGoogle ScholarPubMed
Clarke, H.J. & Masui, Y.. (1986). Transformation of sperm nuclei to the metaphase chromosomes in the cytoplasm of maturing oocytes of the mouse. J. Cell Biol. 102, 1039–46.CrossRefGoogle Scholar
Clarke, H.J. & Masui, Y.. (1987). Does-dependent relationship between oocyte cytoplasmic volume and transformation of sperm nuclei to metaphase chromosomes. J. Cell Biol. 104, 831–40.CrossRefGoogle Scholar
Das, N.K.. Barker, C.. (1976). Mitotic chromosome condensation in the sperm nucleus during postfertilization maturation dicision in Urechis eggs. J. Cell Biol. 68, 155–9.CrossRefGoogle Scholar
Elinson, R.P.. (1977). Fertilization of immature frog eggs: cleavage and development following subsequent activiation. J. Embryol. Exp. Morphol. 37, 187–201.Google Scholar
Kruip, T.A.M., Cran, D.G., Van Beneden, T.H. & Dielemann, S.J.. (1983). Structural changes in bovine oocytes during final maturation in vivo. Gamete Res. 8, 29–47.CrossRefGoogle Scholar
Longo, F.J., Cook, S. & Mathews, L.. (1991). Pronuclear for mation in starfish eggs inseminated at different stages of meiotic maturation: correlation of sperm nuclear transfomations and activity of the maternal chromatin. Dev. Biol. 147, 62–72.CrossRefGoogle Scholar
Masui, Y.. (1985). Problems of oocyte maturation and the control of chromosome cycles. Dev. Growth Differ. 27, 295–309.CrossRefGoogle ScholarPubMed
Masuk, Y. & Markert, C.L.. (1971). Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes. J. Exp. Zool. 177, 129–45.Google Scholar
Meyerhof, P.G. & Masui, Y.. (1977). Ca and Mg control of cytoplasmic factors from Rana pipiens oocytes which cause metaphase and cleavage arrest. Dev. Biol. 61, 214–29.CrossRefGoogle Scholar
Meyhof, P.G.. Masui, Y.. (1979). Chromosome condensation activity in Rana pipiens eggs matured in vitro and in blastomeres arres arrested by cytostatis factor (CSF). Exp. Cell Rse. 123, 345–53.Google Scholar
Moriya, M. & Katagiri, C.. (1976). Microinjection of toad sperm into oocytes undergoing maturation division. Dev. Growth Differ. 18, 349–56.CrossRefGoogle ScholarPubMed
Niwa, K. & Ohgoda, O.. (1988). Synergistic effect of caffeine and heparin on in vitro fertilization of cattle oocytes matured in culture. Theriogenology 30, 733–41.CrossRefGoogle ScholarPubMed
Niwa, K., Park, C.-K. & Okuda, K.. (1991). Penetration in vitro of bovine oocytes during maturation by frozen–thawed spermatozoa. J. Reprod. Fertil. 91, 329–36.CrossRefGoogle ScholarPubMed
Ohgoda, O., Niwa, K., Yuhar, M., Takahashi, S. & Kanoya, K.. (1988). Variation in penetration rates in vitro of bovine follicular oocytes do not reflect conception rates after artificial insemination using frozen semen from different bulls. Theriogenology 29, 1375–81.CrossRefGoogle Scholar
Pincus, G. & Enzmann, E.V.. (1935). The comparative behavior of mammalian eggs in vivo and in vitro. I. The activiation of ovarian eggs. J. Exp. Med. 62, 665–75.CrossRefGoogle Scholar
Saeki, K., Kato, Y., Hosoi, M., Miyake, M., Utsumi, K. & Iritani, A.. (1991). Early morphological events of in vitro fertilized boving oocytes with frozen-thawed spermatozoa. Theriogenology 35, 1051–8.CrossRefGoogle Scholar
Sirard, M.A., Florman, H.M., LeibfriedRutledge, M.L., Barnes, F.L., Sims, M.L. & First, N.L.. (1989). Thming of nuclear progression and protein synthesis necessary for meiotic maturation of boving oocytes. Biol. Reprod. 40, 1257–63.CrossRefGoogle Scholar
Thibault, C. & Gerard, M.. (1973). Cytoplasmic and nuclear maturation of rabbit oocytes in vitro. Ann. Biol. Biochim. Biophys. 13, 145–56.CrossRefGoogle Scholar
Yanagimachi, R.. (1981). Mechanisms of fertilization in mammals. In fertilization and Embryonic Development In Vitro, ed. Mastroianni, L. Jr, & Biggers, J.D., pp. 81–182. New York: Plenum Press.CrossRefGoogle Scholar