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Xenopus interspersed RNA families, Ocr and XR, bind DNA-binding proteins

Published online by Cambridge University Press:  26 September 2008

Katherine Ladner Guttridge  and
L. Dennis Smith
Katherine Ladner Guttridge*
Affiliation:
Developmental Biology Center, University of California-Irvine, Irvine, California, USA
L. Dennis Smith
Affiliation:
Developmental Biology Center, University of California-Irvine, Irvine, California, USA
*
Dr Katherine L. Guttridge, Department of Microbiology and Molecular Genetics, University of California-Irvine, Irvine, CA 92717, USA. Telephone: (714) 725-3096. Fax: (714)856-8598.
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Summary

Interspersed RNA makes up two-thirds of cytoplasmic polyadenylated RNA in Xenopus and sea urchin eggs.Although it has no known function, previous work has suggested that at least one family of interspersed RNA, XR, binds Xenopus oocyte proteins, and can influence the rate of translation. We have used two Xenopus repeat families, Ocr and XR, to explore their protein binding abilities. Ocr RNA binds the same pattern of highly abundant oocyte proteins that XR RNA binds, which are believed to be messenger ribonucleoprotein (mRNP) particle proteins. In addition, we show that Ocr RNA binds the Oct-60 protein, a member of the POU-domain family of transcription factors found in Xenopus oocytes. Using a 32 base pair sequence from the XR repeat in a DNA affinity column two proteins were isolated, 66KDa and 92KDa, that together form a complex with XR DNA. One of these proteins (92KDa) also binds XR RNA. We suggest that the role of at least a subset of interspersed RNAs in development may be to bind, and sequester in the cytoplasm, DNA-binding proteins until the end of oogenesis

Keywords

DNA-binding proteinsInterspersed RNAOogenesisXenopus
Type
Article
Information
Zygote , Volume 3 , Issue 2 , May 1995 , pp. 111 - 122
Copyright
Copyright © Cambridge University Press 1995

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