Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-25T15:07:12.914Z Has data issue: false hasContentIssue false

Ultrastructural changes of Arabidopsis thaliana pollen during final maturation and rehydration

Published online by Cambridge University Press:  26 September 2008

A.C. Van Aelst
Affiliation:
Wageningen Agricultural University, The Netherlands, and University of Siena, Italy.
E.S. Pierson
Affiliation:
Wageningen Agricultural University, The Netherlands, and University of Siena, Italy.
J.L. Van Went
Affiliation:
Wageningen Agricultural University, The Netherlands, and University of Siena, Italy.
M. Cresti*
Affiliation:
Wageningen Agricultural University, The Netherlands, and University of Siena, Italy.
*
M. Cresti, Dipartimento di Biologia Ambientale, Università di Siena, via P.A. Mattioli 4, I-53100 Siena, Italy.

Summary

Several ultrastructural changes occur during dehydration and subsequent rehydration of Arabidopsis thaliana pollen. The cytoplasmic channels, present in the outer part of the intine of the mature, dehydrating pollen grain, degenerate and develop into electron-dense inclusions. At the same time a large quantity of electron-dense material is deposited in the cavities of the exine. A large number of vesicles is produced in the vegetative cell, and they become predominantly located in the peripheral region near the intine. Starch of amyloplasts is consumed and the lipid bodies which originally surround the sperm cells become randomly distributed. In addition, the individual lipid bodies become enveloped by single rough endoplasmic reticulum cisterns.

Type
Article
Copyright
Copyright © Cambridge University Press 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bowman, J.L., Smyth, D.R. & Meyerowitz, E.M. (1989). Genes directing flower development in Arabidopsis. Plant Cell 1, 3752.Google Scholar
Charzynska, M., Murgia, M. & Cresti, M. (1989 a). Ultrastructure of the vegetative cell of Brassica napus pollen with particular reference to microbodies. Protoplasma 152, 22–8.Google Scholar
Charzynska, M., Murgia, M., Milanesi, C. & Cresti, M. (1989 b). Origin of sperm cell association in the ‘male germ unit’ of Brassica pollen. Protoplasma 149, 14.Google Scholar
Cresti, M., Pacini, E., Ciampolini, F. & Sarfatti, G. (1977). Germination and early tube development in vitro of Lycopersicum peruvianum pollen. Planta 136, 239–47.CrossRefGoogle ScholarPubMed
Cresti, M., Ciampolini, F. & Sarfatti, G. (1983). Ultrastructure features of Malus communis L. mature pollen: In Pollen: Biology and Implications for Plant Breeding, ed. Mulcahy, D.L. & Ottaviano, E., pp. 165–72. New York: Elsevier Biomedical.Google Scholar
Cresti, M., Murgia, M. & Theunis, C.H. (1990 a). Microtubule organization in sperm cells in the pollen tubes of Brassica oleracea L. Protoplasma 154, 151–6.Google Scholar
Cresti, M., Milanesi, C., Salvatici, P. & Van Aelst, A.C. (1990 b). Ultrastructural observations of Papaver rhoeas mature pollen grains. Bot. Acta 103, 349–54.CrossRefGoogle Scholar
Gunning, B.E.S. & Steer, M.W. (1986). Plant Cell Biology: An Ultrastructural Approach. London: Edward Arnold.Google Scholar
Heslop-Harrison, J. (1979). Aspects of structure, cytochemistry and germination of the pollen of rye (Secale cereale L.). Ann. Bot. 44 (Suppl), 147.Google Scholar
Heslop-Harrison, J. (1987). Pollen germination and pollen tube growth. Int. Rev. Cytol. 107, 178.Google Scholar
Kuroda, K. (1990). Cytoplasmic streaming in plant cells. Int. Rev. Cytol. 121, 267307.Google Scholar
Mascarenhas, J.P. (1975). The biochemistry of angiosperm pollen development. Bot. Rev. 41, 259314.CrossRefGoogle Scholar
McConchie, C.A., Russell, S.D., Dumas, C. & Knox, R.B. (1987). Quantitative cytology of the sperm cells of Brassica campestris and B. oleracea. Planta 170, 446–52.CrossRefGoogle ScholarPubMed
Miki-Hirosige, H. & Nakamura, S. (1982). Process of metabolism during pollen tube wall formation. J. Electron Microsc. 31, 5162.Google Scholar
Murgia, M., Detchepare, S., Van Went, J.L. & Cresti, M. (1991). Brassica napus pollen development during generative cell and sperm cell formation. Sex. Plant Reprod. 4, 176–81.Google Scholar
Pacini, E., Franchi, G.G. & Hesse, M. (1985). The tapetum: its form, function and possible phylogeny in Embryophyta. Plant Syst. Evol. 149, 155–85.Google Scholar
Pickert, M. (1988). In vitro germination and storage of trinucleate Arabidopsis thaliana (L.) pollen grains. Arabidopsis Inf. Service 3, 3942.Google Scholar
Regan, S.M. & Moffat, B.A. (1990). Cytochemical analysis of pollen development in wild-type Arabidopsis and male-sterile mutant. Plant Cell 2, 877–89.Google Scholar
Twell, D., Yamaguchi, J. & McCormick, S. (1990). Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis. Development 109, 705–13.Google Scholar
Van Aelst, A.C. & Van Went, J.L. (1991). The ultrastructure of mature Papaver dubium L. pollen grains. Acta Bot. Neerl. 40, 319–28.Google Scholar
Van Aelst, A.C. & Van Went, J.L. (1992). Ultrastructural immuno-localization of pectines and glycoproteins in Arabidopsis thaliana pollen grains. Protoplasma 168, 1419.Google Scholar
Van Der Woude, W.J., Morré, D.J. & Bracker, C.E. (1971). Isolation and characterization of secretory vesicles in germinated pollen of Lilium longiflorum. J. Cell Sci. 8, 331–51.Google ScholarPubMed