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Thyroid hormones alter the transcriptome of in vitro-produced bovine blastocysts

Published online by Cambridge University Press:  23 June 2015

Fazl A. Ashkar ,
Tamas Revay ,
NaYoung Rho ,
Pavneesh Madan ,
Isabelle Dufort ,
Claude Robert ,
Laura A. Favetta ,
Chris Schmidt  and
W. Allan King
Fazl A. Ashkar
Affiliation:
Department of Biomedical Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Tamas Revay
Affiliation:
Department of Biomedical Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
NaYoung Rho
Affiliation:
Department of Biomedical Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Pavneesh Madan
Affiliation:
Department of Biomedical Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Isabelle Dufort
Affiliation:
Faculté des Sciences de l'Agriculture et de l'Alimentation, Département des Sciences Animales, Pavillon INAF, Centre de Recherche en Biologie de la Reproduction (CRBR), Université Laval, Québec, Canada.
Claude Robert
Affiliation:
Faculté des Sciences de l'Agriculture et de l'Alimentation, Département des Sciences Animales, Pavillon INAF, Centre de Recherche en Biologie de la Reproduction (CRBR), Université Laval, Québec, Canada.
Laura A. Favetta
Affiliation:
Department of Biomedical Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Chris Schmidt
Affiliation:
Department of Biomedical Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
W. Allan King*
Affiliation:
Department of Biomedical Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
*
All correspondence to: W. Allan King. Department of Biomedical Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada. E-mail: [email protected]
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Summary

Thyroid hormones (THs) have been shown to improve in vitro embryo production in cattle by increasing blastocyst formation rate, and the average cell number of blastocysts and by significantly decreasing apoptosis rate. To better understand those genetic aspects that may underlie enhanced early embryo development in the presence of THs, we characterized the bovine embryonic transcriptome at the blastocyst stage, and examined differential gene expression profiles using a bovine-specific microarray. We found that 1212 genes were differentially expressed in TH-treated embryos when compared with non-treated controls (>1.5-fold at P < 0.05). In addition 23 and eight genes were expressed uniquely in control and treated embryos, respectively. The expression of genes specifically associated with metabolism, mitochondrial function, cell differentiation and development were elevated. However, TH-related genes, including those encoding TH receptors and deiodinases, were not differentially expressed in treated embryos. Furthermore, the over-expression of 52 X-chromosome linked genes in treated embryos suggested a delay or escape from X-inactivation. This study highlights the significant impact of THs on differential gene expression in the early embryo; the identification of TH-responsive genes provides an insight into those regulatory pathways activated during development.

Keywords

BlastocystBovine embryoEmbryonic transcriptomeGene expressionThyroid hormones
Type
Research Article
Information
Zygote , Volume 24 , Issue 2 , April 2016 , pp. 266 - 276
Copyright
Copyright © Cambridge University Press 2015 

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