Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-05T16:12:58.821Z Has data issue: false hasContentIssue false
  • English
  • Français

Sulphydryl reagent iodoacetamide inhibits progression of meiosis and sperm transformation in mouse oocytes fertilised in vitro

Published online by Cambridge University Press:  26 September 2008

Marek Maleszewski
Marek Maleszewski
Affiliation:
Department of Embryology, Institute of Zoology, University of Warsaw, Poland.
Get access Rights & Permissions [Opens in a new window]

Summary

The effect of iodoacetamide (IA), a sulphydryl blocking agent, on fertilisation in the mouse was examined by transferring zona-free oocytes into IA (500 μM)-containing medium at various times after insemination. When inseminated oocytes were transferred into IA medium 10 min after insemination, the oocyte chromosomes remained aggregated in one or two masses and the sperm nucleus failed to decondense. When oocytes were transferred during the second meiosis, oocytes meiosis was more or less arrested. The sperm nucleus decondensed but remained blocked at an early stage of decondensation. These observations suggest that thiol groups in the oocyte's cytoplasm and perhaps microtubules of the meiotic spindle play crucial roles in the completion of meiosis and the transformation of sperm nucleus into pronucleus.

Keywords

Disulphide bondsFertilisation in vitroIodoacetamideOocyte activationSperm transformation
Type
Article
Information
Zygote , Volume 3 , Issue 1 , February 1995 , pp. 75 - 79
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abramson, J.J., Trimm, J.L., & Salama, G.. (1983) Heavy metals induce rapid calcium release from sarcoplasmic reticulum vesicles isolated from skeletal muscle. Proc. Natl. Acad. Sci. USA 80, 1526–30.CrossRefGoogle ScholarPubMed
Adenot, P.G., Szöllösi, M.S., Geze, M., Renard, J-P, & Debey, P. (1991). Dynamics of paternal chromatin changes in live one-cell mouse embryo after natural fertilization. Mol. Reprod. Dev. 28, 23–34.CrossRefGoogle ScholarPubMed
Dale, B., & Russo, P.. (1988). Sulfhydryl groups are involved in the activation of sea urchin eggs. Gamete Res. 19, 161– 8.CrossRefGoogle ScholarPubMed
Fraser, L.R., & Drury, L.M.. (1975). The relationship between sperm concentration and fertilization in vitro of mouse eggs. Biol. Reprod. 13, 513–18.CrossRefGoogle ScholarPubMed
Fulton, B.P., & Whittingham, D.G.. (1978). Activation of mammalian oocytes by intracellular injection of calcium. Nature 273, 149–51.CrossRefGoogle ScholarPubMed
Kishimoto, T., & Kanatani, H.. (1973). Induction of starfish oocyte maturation by disulfide-reducing agents. Exp. Cell Res. 101, 296–302.CrossRefGoogle Scholar
Krzanowska, H.. (1982). Toluidine blue staining reveals changes in chromation stabilization of mouse spermatozoa during epididymal maturation and penetration of ova. J. Reprod. Fertil. 64, 97–101.CrossRefGoogle Scholar
Lassalle, B., & Testart, J.. (1989). Effects of glutathione (GSH and GSSG) and glutathione reductase (GR) on zona-free hamster oocyte ability to decondense human sperm. Gamete Res. 24. 21–30.CrossRefGoogle ScholarPubMed
Maleszewski, M.. (1990). Decondensation of mouse sperm chromatin in cell-free extracts: a micromethod. Mol. Reprod. Dev. 27 244–8.CrossRefGoogle ScholarPubMed
Mellon, M.G., & Rebhun, L.I.. (1976). Sulfhydryls and in vitro polymerization of tubulin. J. Cell Biol. 70, 226–38.CrossRefGoogle ScholarPubMed
Miller, M.A., & Masui, Y.. (1982). Changes in the stainability and sulphydryl level in the sperm nucleus during sperm-oocyte interaction in mice. Gamete Res. 5, 167–79.CrossRefGoogle Scholar
Olivier, J.M., Albertini, D.F., & Berlin, R.D.. (1976). Effects of glutathione-oxidizing agents on microtubule assembly and microtubule-dependent surface properties of human neutrophils. J. Cell Biol. 71, 921–32.CrossRefGoogle Scholar
Olivier, J.M., Spielberg, S.P., Pearson, C.B., & Schulman, J.D. (1978). Microtubule assembly and function in normal and glutathione synthetase-deficient polymorphonuclear leu-kocytes. j. Immunol. 120, 1181–6.CrossRefGoogle Scholar
Perreault, S.D., Barbee, R.R., & Slott, V.L.. (1988). Importance of glutathione in the acquisition and maintenance of sperm nuclear decondensing activity in maturing hamster oocytes. Dev. Biol. 125. 181–6.CrossRefGoogle ScholarPubMed
Perreault, S.D., Wolff, R.A., & Zirkin, B.. (1984). The role of disulfide bonds reduction during mammalian sperm nuclear decondensation in vitro. Dev. Biol. 101, 160–7.CrossRefGoogle Scholar
Usui, N., & Yanagimachi, R.. (1976). Behavior of hamster sperm nuclei incorporated into eggs at various stages of maturation, fertilization and early development. J. Ultra-struct. Res. 57, 276–88.CrossRefGoogle ScholarPubMed
Whittingham, D.G.. (1971). Culture of mouse ova. Biol. Reprod. (Suppl.) 2, 44–63.Google Scholar
Yanagimachi, R.. (1994). Mammalian fertilization. In: The Physiology of Reproduction. ed Knobil, E. & Neill, J.D., pp. 189–317. New York: Raven Press.Google Scholar
Zirkin, B.R.,. Perreault, S.D., & Nasih, S. (1989). Formation and function of the male pronucleus during mammalian fertilization. In the Molecular Biology of Fertilization, ed Schatten, H. & Schatten, G.. pp. 91–114. Orlando, FL: Aca demic Press.CrossRefGoogle Scholar
Zirkin, B.R., Soucek, D.A., Chang, T.S.K., & Perrault, S.D. (1985). In vitro and in vivo studies of mammalian sperm nuclear decondensation. Gamete Res. 11, 349–65.CrossRefGoogle Scholar