Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-23T12:53:02.042Z Has data issue: false hasContentIssue false
  • English
  • Français

Successful birth after ICSI with testicular immotile spermatozoa from a patient with total MMAF in the ejaculates: a case report

Published online by Cambridge University Press:  29 September 2021

Liuguang Zhang Open the ORCID record for Liuguang Zhang [Opens in a new window] ,
Yuhu Li ,
Yuqun Huang  and
Zongqiang Li
Liuguang Zhang*
Affiliation:
Center for Reproductive Medicine, Haikou Mary Hospital, 7 Lantian Road, Haikou570203, China
Yuhu Li
Affiliation:
Center for Reproductive Medicine, Haikou Mary Hospital, 7 Lantian Road, Haikou570203, China
Yuqun Huang
Affiliation:
Dong Guan Guang Ji Hospital, Dong Guan, 523690, China
Zongqiang Li
Affiliation:
College of Animal Science and Technology, Guang Xi University, NanNing 530004, Guang Xi, China
*
Author for correspondence: Liuguang Zhang. Center for Reproductive Medicine, Haikou Mary Hospital, 7 Lantian Road, Haikou570203, China. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

There has been no report on the outcome of vitrified blastocyst transfer from a vitrified oocyte injected with immotile testicular spermatozoa with only multiple morphological abnormalities of the sperm flagella (MMAF). A couple diagnosed with MMAF returned to the clinic to attempt pregnancy using their vitrified oocytes. Testicular spermatozoa were injected intracytoplasmically, and the following intracytoplasmic sperm injection results were observed. In the second cycle, surplus vitrified oocytes and testicular retrieved sperm were used, but no pregnancy ensued. In the third cycle, a surplus vitrified blastocyst was transferred, and a healthy female child was delivered, with a birth weight of 3050 g and a birth length of 53 cm. In this report we describe a successful pregnancy achieved in a patient presenting MMAF. The successful pregnancy was obtained from vitrified oocytes microinjected with testicular retrieved sperm in a vitrified blastocyst transfer.

Keywords

Multiple morphological abnormalities of the sperm flagellaTesticular spermatozoaVitrified blastocystsVitrified oocytes
Type
Review Article
Information
Zygote , Volume 30 , Issue 2 , April 2022 , pp. 169 - 175
Copyright
© The Author(s), 2021. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

These authors contributed equally to this work.

References

Afzelius, BA, Dallai, R, Lanzavecchia, S and Bellon, PL (1995). Flagellar structure in normal human spermatozoa and in spermatozoa that lack dynein arms. Tissue Cell 27, 241–7.Google ScholarPubMed
Awaga, HA, Bosdou, JK, Goulis, DG, Chatzimeletiou, K, Salem, M, Roshdy, S, Grimbizis, G, Tarlatzis, BC and Kolibianakis, EM (2018). Testicular versus ejaculated spermatozoa for ICSI in patients without azoospermia: A systematic review. Reprod Biomed Online 37, 573–80.Google ScholarPubMed
Barros, A, Sousa, M, Oliveira, C, Silva, J, Almeida, V and Beires, J (1997). Pregnancy and birth after intracytoplasmic sperm injection with totally immotile sperm recovered from the ejaculate. Fertil Steril 67, 1091–4.CrossRefGoogle ScholarPubMed
Barthelemy, C, Tharanne, MJ, Lebos, C, Lecomte, P and Lansac, J (1990). Tail stump spermatozoa: Morphogenesis of the defect. An ultrastructural study of sperm and testicular biopsy. Andrologia 22, 417–25.CrossRefGoogle ScholarPubMed
Ben Khelifa, M, Coutton, C, Zouari, R, Karaouzène, T, Rendu, J, Bidart, M, Yassine, S, Pierre, V, Delaroche, J, Hennebicq, S, Grunwald, D, Escalier, D, Pernet-Gallay, K, Jouk, PS, Thierry-Mieg, N, Touré, A, Arnoult, C and Ray, PF (2014). Mutations in DNAH1, which encodes an inner arm heavy chain dynein, lead to male infertility from multiple morphological abnormalities of the sperm flagella. Am J Hum Genet 94, 95–104.CrossRefGoogle ScholarPubMed
Casper, RF, Meriano, JS, Jarvi, KA, Cowan, L and Lucato, ML (1996). The hypo-osmotic swelling test for selection of viable sperm for intracytoplasmic sperm injection in men with complete asthenozoospermia. Fertil Steril 65, 972–6.CrossRefGoogle ScholarPubMed
Chemes, HE (2012). Sperm centrioles and their dual role in flagellogenesis and cell cycle of the zygote. In: Schatten, H. (ed.), The Centrosome, pp. 33–48. Humana Press.Google Scholar
Chemes, HE, Brugo, S, Zanchetti, F, Carrere, C and Lavieri, JC (1987). Dysplasia of the fibrous sheath: An ultrastructural defect of human spermatozoa associated with sperm immotility and primary sterility. Fertil Steril 48, 664–9.Google ScholarPubMed
Coutton, C, Escoffier, J, Martinez, G, Arnoult, C and Ray, PF (2015). Teratozoospermia: Spotlight on the main genetic actors in the human. Hum Reprod Update 21, 455–85.Google ScholarPubMed
Coutton, C, Vargas, AS, Amiri-Yekta, A, Kherraf, ZE, Ben Mustapha, SF, Le Tanno, P, Wambergue-Legrand, C, Karaouzène, T, Martinez, G, Crouzy, S, Daneshipour, A, Hosseini, SH, Mitchell, V, Halouani, L, Marrakchi, O, Makni, M, Latrous, H, Kharouf, M et al. (2018). Mutations in CFAP43 and CFAP44 cause male infertility and flagellum defects in Trypanosoma and human. Nat Commun 9, 686.CrossRefGoogle ScholarPubMed
Craft, I and Tsirigotis, M (1995). Simplified recovery, preparation and cryopreservation of testicular spermatozoa. Hum Reprod 10, 1623–6.Google ScholarPubMed
Dávila Garza, SA and Patrizio, P (2013). Reproductive outcomes in patients with male infertility because of Klinefelter’s syndrome, Kartagener’s syndrome, round-head sperm, dysplasia fibrous sheath, and ‘stump’ tail sperm: An updated literature review. Curr Opin Obstet Gynecol 25, 229–46.Google Scholar
Dong, FN, Amiri-Yekta, A, Martinez, G, Saut, A, Tek, J, Stouvenel, L, Lorès, P, Karaouzène, T, Thierry-Mieg, N, Satre, V, Brouillet, S, Daneshipour, A, Hosseini, SH, Bonhivers, M, Gourabi, H, Dulioust, E, Arnoult, C, Touré, A et al. (2018). Absence of CFAP69 causes male infertility due to multiple morphological abnormalities of the flagella in human and mouse. Am J Hum Genet 102, 636–48.Google ScholarPubMed
Esteves, SC, Miyaoka, R, Orosz, JE and Agarwal, A (2013). An update on sperm retrieval techniques for azoospermic males. Clinics 68, Suppl. 1, 99–110.CrossRefGoogle Scholar
Fauque, P, Albert, M, Serres, C, Viallon, V, Davy, C, Epelboin, S, Chalas, C, Jouannet, P and Patrat, C (2009). From ultrastructural flagellar sperm defects to the health of babies conceived by ICSI. Reprod Biomed Online 19, 326–36.CrossRefGoogle Scholar
Gardner, DK, Lane, M, Stevens, J, Schlenker, T and Schoolcraft, WB (2000). Blastocyst score affects implantation and pregnancy outcome: Towards a single blastocyst transfer. Fertil Steril 73, 1155–8.Google ScholarPubMed
Huang, T, Yin, Y, Liu, C, Li, M, Yu, X, Wang, X, Zhang, H, Muhammad, T, Gao, F, Li, W, Chen, Z, Liu, H and Ma, J (2020). Absence of murine CFAP61 causes male infertility due to multiple morphological abnormalities of the flagella. Sci Bull 65, 854–64.Google Scholar
Hyun, CS, Cha, JH, Son, WY, Yoon, SH, Kim, KA and Lim, JH (2007). Optimal ICSI timing after the first polar body extrusion in in vitro matured human oocytes. Hum Reprod 22, 1991–5.CrossRefGoogle ScholarPubMed
Inaba, K (2007). Molecular basis of sperm flagellar axonemes: Structural and evolutionary aspects. Ann NY Acad Sci 1101, 506–26.Google ScholarPubMed
Lorès, P, Coutton, C, El Khouri, E, Stouvenel, L, Givelet, M, Thomas, L, Rode, B, Schmitt, A, Louis, B, Sakheli, Z, Chaudhry, M, Fernandez-Gonzales, A, Mitsialis, A, Dacheux, D, Wolf, JP, Papon, JF, Gacon, G, Escudier, E et al. (2018). Homozygous missense mutation L673P in adenylate kinase 7 (AK7) leads to primary male infertility and multiple morphological anomalies of the flagella but not to primary ciliary dyskinesia. Hum Mol Genet 27, 1196–211.CrossRefGoogle ScholarPubMed
Liu, C, Lv, M, He, X, Zhu, Y, Amiri-Yekta, A, Li, W, Wu, H, Kherraf, ZE, Liu, W, Zhang, J, Tan, Q, Tang, S, Zhu, YJ, Zhong, Y, Li, C, Tian, S, Zhang, Z, Jin, L et al. (2020a). Homozygous mutations in SPEF2 induce multiple morphological abnormalities of the sperm flagella and male infertility. J Med Genet 57, 31–7.CrossRefGoogle ScholarPubMed
Liu, C, Miyata, H, Gao, Y, Sha, Y, Tang, S, Xu, Z, Whitfield, M, Patrat, C, Wu, H, Dulioust, E, Tian, S, Shimada, K, Cong, J, Noda, T, Li, H, Morohoshi, A, Cazin, C, Kherraf, ZE et al. (2020b). Bi-allelic DNAH8 variants lead to multiple morphological abnormalities of the sperm flagella and primary male infertility. Am J Hum Genet 107, 330–41.CrossRefGoogle Scholar
McLachlan, RI, Ishikawa, T, Osianlis, T, Robinson, P, Merriner, DJ, Healy, D, de Kretser, D and O’Bryan, MK (2012). Normal live birth after testicular sperm extraction and intracytoplasmic sperm injection in variant primary ciliary dyskinesia with completely immotile sperm and structurally abnormal sperm tails. Fertil Steril 97, 313–8.Google ScholarPubMed
Mitchell, V, Rives, N, Albert, M, Peers, MC, Selva, J, Clavier, B, Escudier, E and Escalier, D (2006). Outcome of ICSI with ejaculated spermatozoa in a series of men with distinct ultrastructural flagellar abnormalities. Hum Reprod 21, 2065–74.CrossRefGoogle Scholar
Mortimer, D (2018). The functional anatomy of the human spermatozoon: Relating ultrastructure and function. Mol Hum Reprod 24, 567–92.Google ScholarPubMed
Nagy, ZP, Varghese, AC and Agarwal, A (2019). In Vitro Fertilization. A Textbook of Current and Emerging Methods and Devices. Springer International Publishing.CrossRefGoogle Scholar
Nijs, M, Vanderzwalmen, P, Vandamme, B, Segal-Bertin, G, Lejeune, B, Segal, L, van Roosendaal, E and Schoysman, R (1996). Fertilizing ability of immotile spermatozoa after intracytoplasmic sperm injection.Hum Reprod 11, 2180–5.Google ScholarPubMed
Nsota Mbango, JF, Coutton, C, Arnoult, C, Ray, PF and Touré, A (2019). Genetic causes of male infertility: Snapshot on morphological abnormalities of the sperm flagellum. Basic Clin Androl 29, 2.Google ScholarPubMed
Palermo, GD, Schlegel, PN, Sills, ES, Veeck, LL, Zaninovic, N, Menendez, S and Rosenwaks, Z (1998). Births after intracytoplasmic injection of sperm obtained by testicular extraction from men with nonmosaic Klinefelter’s syndrome. New Engl J Med 338, 588–90.CrossRefGoogle ScholarPubMed
Peeraer, K, Nijs, M, Raick, D and Ombelet, W (2004). Pregnancy after ICSI with ejaculated immotile spermatozoa from a patient with immotile cilia syndrome: A case report and review of the literature. Reprod Biomed Online 9, 659–63.CrossRefGoogle ScholarPubMed
Sallam, HN, Farrag, A, Aganeya, AF, Ezzeldin F Eid A and Sallam A (2005). The use of a modified hypo-osmotic swelling test for the selection of viable ejaculated and testicular immobile spermatozoa in ICSI. Hum Reprod 16, 272–6.CrossRefGoogle Scholar
Sathananthan, AH (1994). Functional competence of abnormal spermatozoa. Baillieres Clin Obstet Gynaecol 8, 141–56.CrossRefGoogle ScholarPubMed
Sciorio, R and Smith, GD (2019). Embryo culture at a reduced oxygen concentration of 5%: A mini review. Zygote 27, 355–61.CrossRefGoogle Scholar
Sha, YW, Xu, X, Mei, LB, Li, P, Su, ZY, He, XQ and Li, L (2017). A homozygous CEP135 mutation is associated with multiple morphological abnormalities of the sperm flagella (MMAF). Gene 633, 48–53.Google Scholar
Sha, Y, Wei, X, Ding, L, Ji, Z, Mei, L, Huang, X, Su, Z, Wang, W, Zhang, X and Lin, S (2020a). Biallelic mutations of CFAP74 may cause human primary ciliary dyskinesia and MMAF phenotype. J Hum Genet 65, 961–9.Google ScholarPubMed
Sha, Y, Wei, X, Ding, L, Mei, L, Huang, X, Lin, S, Su, Z, Kong, L, Zhang, Y and Ji, Z (2020b). DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella. Ann Hum Genet 84, 271–9.Google ScholarPubMed
Shen, Y, Zhang, F, Li, F, Jiang, X, Yang, Y, Li, X, Li, W, Wang, X, Cheng, J, Liu, M, Zhang, X, Yuan, G, Pei, X, Cai, K, Hu, F, Sun, J, Yan, L, Tang, L et al. (2019). Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella. Nat Commun 10, 433.Google ScholarPubMed
Stalf, T, Sánchez, R, Köhn, FM, Schalles, U, Kleinstein, J, Hinz, V, Tielsch, J, Khanaga, O, Turley, H and Gips, H (1995). Pregnancy and birth after intracytoplasmic sperm injection with spermatozoa from a patient with tail stump syndrome. Hum Reprod 10, 2112–2114.Google ScholarPubMed
Stanger, JD, Vo, L, Yovich, JL and Almahbobi, G (2010). Hypo-osmotic swelling test identifies individual spermatozoa with minimal DNA fragmentation. Reprod Biomed Online 21, 474–84.CrossRefGoogle Scholar
Talreja, D, Gupta, C, Pai, H and Palshetkar, N (2020). Oocyte vitrification: A comparative analysis between fresh and cryopreserved oocytes in an oocyte donation program. Fertil Reprod 2, 9–13.Google Scholar
Tesarik, J and Sousa, M (1995). Key elements of a highly efficient intracytoplasmic sperm injection technique: Ca2+ fluxes and oocyte cytoplasmic dislocation. Fertil Steril 64, 770–6.CrossRefGoogle ScholarPubMed
Van Blerkom, J (1996). Sperm centrosome dysfunction: A possible new class of male factor infertility in the human. Mol Hum Reprod 2, 349–54.CrossRefGoogle ScholarPubMed
Vandervorst, M, Liebaers, I, Sermon, K, Staessen, C, De Vos, A, Van de Velde, H, Van Assche, E, Joris, H, Van Steirteghem, A and Devroey, P (1998). Successful preimplantation genetic diagnosis is related to the number of available cumulus–oocyte complexes. Hum Reprod 13, 3169–76.Google Scholar
Viville, S, Mollard, R, Bach, ML, Falquet, C, Gerlinger, P and Warter, S (2000). Do morphological anomalies reflect chromosomal aneuploidies?: Case report. Hum Reprod 15, 2563–6.CrossRefGoogle ScholarPubMed
Wambergue, C, Zouari, R, Fourati Ben Mustapha, S, Martinez, G, Devillard, F, Hennebicq, S, Satre, V, Brouillet, S, Halouani, L, Marrakchi, O, Makni, M, Latrous, H, Kharouf, M, Amblard, F, Arnoult, C, Ray, PF and Coutton, C (2016). Patients with multiple morphological abnormalities of the sperm flagella due to DNAH1 mutations have a good prognosis following intracytoplasmic sperm injection. Hum Reprod 31, 1164–72.CrossRefGoogle ScholarPubMed
Wang, WL, Tu, CF and Tan, YQ (2020). Insight on multiple morphological abnormalities of sperm flagella in male infertility: What is new? Asian J Androl 22, 236–45.Google Scholar
World Health Organization (2010). WHO Laboratory manual for the examination and processing of human semen (5th edn). World Health Organization. ISBN: 9789241547789Google Scholar
Yang, SM, Li, HB, Wang, JX, Shi, YC, Cheng, HB, Wang, W, Li, H, Hou, JQ and Wen, DG (2015). Morphological characteristics and initial genetic study of multiple morphological anomalies of the flagella in China. Asian J Androl 17, 513–5.Google ScholarPubMed
Yang, L, Banerjee, S, Cao, J, Bai, X, Peng, Z, Chen, H, Huang, H, Han, P, Feng, S, Yi, N, Song, X and Wu, J (2018). Compound heterozygous variants in the coiled-coil domain containing 40 gene in a Chinese family with primary ciliary dyskinesia cause extreme phenotypic diversity in cilia ultrastructure. Front Genet 9, 23.CrossRefGoogle Scholar
Yang, S, Gao, L, Wang, W, Ding, J, Xu, Y and Li, H (2018). Successful intracytoplasmic sperm injection with testicular spermatozoa from a man with multiple morphological abnormalities of the sperm flagella: A case report. J Assist Reprod Genet 35, 247–50.CrossRefGoogle ScholarPubMed