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Spermatozoa isolated from cat testes retain their structural integrity as well as a developmental potential after refrigeration for up to 7 days

Published online by Cambridge University Press:  03 July 2014

Sirirak Buarpung ,
Theerawat Tharasanit ,
Chommanart Thongkittidilok ,
Pierre Comizzoli  and
Mongkol Techakumphu
Sirirak Buarpung
Affiliation:
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Theerawat Tharasanit
Affiliation:
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Chommanart Thongkittidilok
Affiliation:
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Pierre Comizzoli
Affiliation:
Department of Reproductive Sciences, Center for Species Survival, Smithsonian Conservation Biology Institute, Washington, District of Columbia, USA.
Mongkol Techakumphu*
Affiliation:
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
*
All correspondence to: Mongkol Techakumphu. Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. Tel: +66 2 218 9644. Fax: +66 2 2520738. e-mail: [email protected]
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Summary

The objective of this study was to compare the efficiency of preservation media for isolated feline testicular spermatozoa as well as the concentrations of bovine serum albumin (BSA) on: (1) the membrane (sperm membrane integrity (SMI)) and DNA integrity of spermatozoa; and (2) the developmental potential of spermatozoa after intracytoplasmic sperm injection (ICSI). Isolated cat spermatozoa were stored in HEPES-M199 medium (HM) or Dulbecco's phosphate-buffered saline (DPBS) at 4°C for up to 7 days. Results indicated that HM maintained a better SMI than DPBS throughout the storage periods (P > 0.05). When spermatozoa were stored in HM supplemented with BSA at different concentrations (4, 8 or 16 mg/ml), SMI obtained from HM containing 8 and 16 mg/ml BSA was higher than with 4 mg/ml BSA (P < 0.05). DNA integrity of spermatozoa stored in HM with 16 mg/ml BSA for 7 days was poorer than that of the fresh control, but the subsequent percentages of cleavage, morula, blastocyst produced by ICSI, as well as their average blastomere numbers of blastocysts, were similar (P > 0.05). In summary, cat spermatozoa immediately isolated from testicular tissue can be stored as a suspension in basic buffered medium at 4°C for up to 7 days. BSA supplementation into the medium improves membrane integrity of the spermatozoa during cold storage. Testicular spermatozoa stored in HM containing 16 mg/ml BSA retained full in vitro developmental potential after ICSI, similar to that of fresh controls even though DNA integrity had slightly declined.

Keywords

Cold storageDNA integrityICSIMembrane integrityTesticular spermatozoa
Type
Research Article
Information
Zygote , Volume 23 , Issue 5 , October 2015 , pp. 644 - 651
Copyright
Copyright © Cambridge University Press 2014 

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