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Sperm penetration in parthenogenetic mouse embryos triggers a plasma membrane block to polyspermy

Published online by Cambridge University Press:  26 September 2008

Marek Maleszewski  and
Anna Bielak
Marek Maleszewski*
Affiliation:
Institute of Zoology, University of Warsaw, Warsaw, Poland
Anna Bielak
Affiliation:
Institute of Zoology, University of Warsaw, Warsaw, Poland
*
Marek Maleszewski, Department of Embryology, Institute of Zoology, University of Warsaw, 00-927 Warsaw 64, Poland. Fax: (48 22) 26 86 24.
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Summary

Mouse oocytes activated parthenogenetically do not generate a plasma membrane block against spermatozoa over the first three cell cycles. We show that they lose this fusibility spontaneously at the 8-cell stage. Insemination of 1-cell parthenogenetic embryos induces loss of fusibility earlier, at the 2-cell stage. This observation suggests that incorporation of the sperm cell membrane components into the oolemma may be responsible for the development of the membrane block.

Keywords

Block to polyspermyIn vitro fertilisationEgg plasma membraneParthenogenetic embryoSperm fusion
Type
Review Article
Information
Zygote , Volume 1 , Issue 3 , August 1993 , pp. 237 - 242
Copyright
Copyright © Cambridge University Press 1993

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References

Austin, C.R. & Braden, A.W.H. (1954). Induction and inhibition of the second polar division in the rat egg and subsequent fertilization. Aust. J. Biol. Sci. 7, 195–210.Google Scholar
Calarco, P.G. (1991). Fertilization in the mouse oocytes. J. Electron Microsc. Technique 17, 401–11.CrossRefGoogle Scholar
Cherr, G.N., Drobnis, E.Z. & Katz, D.F., (1988). Localization of cortical granule constituents before and after exocytosis in the hamster egg. J. Exp. Zool. 246, 81–93.Google Scholar
Cuthbertson, K.S.R. (1983). Parthenogenetic activation of mouse oocytes in vitro with ethanol and benzyl alkohol. J. Exp. Zool. 226, 311–14.CrossRefGoogle Scholar
Fraser, L.R. (1982). Ca2+ is required for mouse sperm capacitation and fertilization in vitro. J. Androl. 3, 412–19.Google Scholar
Fraser, L.R. & Drury, L.M., (1975). The relationship between sperm concentration and fertilization in vitro of mouse eggs. Biol. Reprod. 13, 513–18.CrossRefGoogle ScholarPubMed
Fulton, B.P. & Whittingham, D.G., (1978). Activation of mammalian oocytes by intracellular injection of calcium. Nature 273, 149–51.Google Scholar
Gabel, C.A., Eddy, E.M. & Shapiro, B.M. (1979). After fertilization, sperm surface components remain as a patch in sea urchin and mouse embryos. Cell 18, 207–15.Google Scholar
Gaunt, S.J. (1983). Spreading of a sperm surface antigen within the plasma membrane of the egg after fertilization of the rat. J. Embryol. Exp. Morphol. 75, 259–70.Google ScholarPubMed
Gulyas, B.J. & Yuan, L.C. (1985). Cortical reaction and zona hardening in mouse oocytes following exposure to ethanol. J. Exp. Zool. 233, 269–76.CrossRefGoogle Scholar
Gundersen, G.G., Gabel, C.A. & Shapiro, B.M. (1982). An intermediate state of fertilization involved in internalization of sperm components. Dev. Biol. 93, 59–72.CrossRefGoogle ScholarPubMed
Horvath, P.M., Kellom, T., Caulfield, J. & Boldt, J. (1993). Mechanistic studies of the plasma membrane block to polyspermy in mouse eggs. Mol. Reprod. Dev. 34, 65–72.Google Scholar
Johnson, L. & Calarco, P. (1980). Electrophoretic analysis of cell surface proteins of preimplantation mouse embryos. Dev. Biol. 77, 224–7.CrossRefGoogle ScholarPubMed
Lee, S.H., Ahuja, K.K., Gilburt, D.J. & Whittingham, D.G. (1988). The appearance of glycoconjugates associated with cortical granule release during mouse fertilization. Development 105, 595–604.Google Scholar
Maleszewski, M. (1990). Decondensation of mouse sperm chromatin in cell-free extracts: a micromethod. Mol. Reprod. Dev. 27, 244–8.CrossRefGoogle ScholarPubMed
Maleszewski, M. (1992). Behavior of sperm nuclei incorporated into parthenogenetic mouse eggs prior to the first cleavage division. Mol. Reprod. Dev. 33, 215–21.CrossRefGoogle Scholar
Menezo, Y. & Khatchadourian, C. (1990). Implication de l'activitéglucose-6-phosphate isomerase (EC 5.3.1.9) dans l'arrêt de la segmentation de l'oeuf de souris au stade 2 cellules in vitro. C. R. Acad. Sci. Paris 310, 297–301.Google Scholar
Menge, A. & Black, C. (1979). Effects of antisera on human sperm penetration of zona-free hamster ova. Fertil. Steril. 32, 214–18.CrossRefGoogle ScholarPubMed
Miller, M.A. & Masui, Y. (1982). Changes in the stainability and sulphydryl level in the sperm nucleus during sperm oocyte interaction in mice. Gamete Res. 5, 167–79.Google Scholar
Nicolson, G.I., Yanagimachi, R. & Yanagimachi, H. (1975). Ultrastructural localization of lectin-binding sites on the zonae pellucidae and plasma membranes of mammalian eggs. J. Cell Biol. 66, 263–74.Google Scholar
Okabe, M., Yagasaki, M., Oda, H., Matzno, S., Kohama, Y. & Mimura, T. (1988). Effect of a monoclonal anti–mouse sperm antibody (OBF-13) on the interaction of mouse sperm with zona–free mouse and hamster eggs. J. Reprod. Immunol 13, 211–19.Google Scholar
Primakoff, P., Hyatt, H. & Tredick-Line, J. (1987). Identification and purification of a sperm surface protein with a potntial role in sperm-egg membrane fusion. J. Cell Biol. 104, 141–9.CrossRefGoogle Scholar
Saling, P., Irons, G. & Waibel, R. (1985). Mouse sperm antigens that participate in fertilization. I. Inhibition of sperm fusion with the egg plasma membrane using monoclonal antibodies. Biol. Reprod. 33, 515–26.Google Scholar
Szöllösi, D. (1967). Development of cortical granules end the cortical reaction in rat and hamster eggs. Anat. Rec. 159, 432–45.Google Scholar
Tarkowski, A.K. (1966). An air-drying method for chromosome preparation from mouse eggs. Cytogenetics 5, 394–400.CrossRefGoogle Scholar
Usui, N. & Yanagimachi, R. (1976). Behavior of hamster sperm nuclei incorporated into eggs at various stages of maturation, fertilization and early development: the appearance and disappearance of factors involved in sperm chromatin decondensation in egg cytoplasm. J. Ultrastruct. Res. 57. 276–88CrossRefGoogle ScholarPubMed
Wassarman, P. (1990). Profile of mammalian sperm receptor. Development 108, 1–17.Google Scholar
Whittingham, D.G. (1971). Culture of mouse ova. J. Reprod. Fertil. (Suppl.) 14, 7–21.Google Scholar
Wolf, D.P. (1978). The block to sperm penetration in zona-free mouse eggs. Dev. Biol. 64, 1–10.CrossRefGoogle Scholar
Wolf, D.P., Inoue, M. & Stark, R. (1976). Penetration of zona-free mouse ova. Biol. Reprod. 15, 214–21.Google Scholar
Wolf, D.P., Nicosia, S.V. & Hamada, M. (1979). Premature cortical granule loss does not prevent sperm penetration of mouse eggs. Dev. Biol. 71, 22–32.Google Scholar
Yanagimachi, R. (1981). Mechanisms of fertilization in mammals. In: Fertilization and Embryonic Development In Vitro, Mastroianni, L. & Biggers, J., 81–182. New York: Plenum Press.Google Scholar
Zuccotti, M., Yanagimachi, R. & Yanagimachi, H. (1991). The ability of hamster oolemma to fuse with spermatozoa: its acquisition during oogenesis and loss after fertilization. Development 112, 143–52.Google Scholar