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A specific adenylyl cyclase inhibitor (DDA) and a cyclic AMP-dependent protein kinase inhibitor (H-89) block the action of equine growth hormone on in vitro maturation of equine oocytes

Published online by Cambridge University Press:  26 September 2014

Gabriel Ribas Pereira ,
Pedro Luis Lorenzo ,
Gustavo Ferrer Carneiro ,
Sylvie Bilodeau-Goeseels ,
John Kastelic  and
Irwin K. M. Liu
Gabriel Ribas Pereira*
Affiliation:
Animal Science Department, School of Agronomy, Federal University of Rio Grande do Sul, Campus Agronomia, Ave. Bento Gonçalves 7712, Porto Alegre, 91540–000, RS, Brasil. Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA, USA.
Pedro Luis Lorenzo
Affiliation:
Animal Physiology Department, Veterinary School, Universidad Complutense de Madrid, Madrid, Spain.
Gustavo Ferrer Carneiro
Affiliation:
Animal Reproduction Department, Garanhuns Academic Unity, Federal Rural University of Pernambuco, Garanhuns, PE, Brazil.
Sylvie Bilodeau-Goeseels
Affiliation:
Agriculture and Agri-Food Canada Research Centre, Lethbridge, Canada.
John Kastelic
Affiliation:
Agriculture and Agri-Food Canada Research Centre, Lethbridge, Canada.
Irwin K. M. Liu
Affiliation:
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA, USA.
*
All correspondence to: Gabriel Ribas Pereira. Animal Science Department, School of Agronomy, Federal University of Rio Grande do Sul, Campus Agronomia, Ave. Bento Gonçalves 7712, Porto Alegre, 91540–000, RS, Brasil. Tel.: +55 55 99972240 or +55 51.33086958. e-mail: [email protected] or [email protected]
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Summary

The objectives of this study were firstly to determine whether the stimulatory function of equine growth hormone (eGH) on equine oocyte maturation in vitro is mediated via cyclic adenosine monophosphate (cAMP); and secondly if the addition of eGH in vitro influences oocyte nuclear maturation and if this effect is removed when GH inhibitors are added to the culture. Cumulus–oocyte complexes (COCs) were recovered from follicles <25 mm in diameter and randomly allocated as follows: (i) control (no additives); and (ii) 400 ng/ml of eGH. A specific inhibitor against cyclic AMP-dependent protein kinase (H-89; 10−9, 10−11 or 10−15 M concentration) and a specific adenylate cyclase inhibitor, 2′,3′-dideoxyadenosine (DDA; 10−8, 10−10 or 10−14 M concentration) were used to observe whether they could block the eGH effect. After 30 h of in vitro maturation at 38.5°C with 5% CO2 in air, oocytes were stained with 10 μg/ml of Hoechst to evaluate nuclear status. More mature oocytes (P < 0.05) were detected when COCs were incubated with eGH (29 of 84; 34.5%) than in the control group (18 of 82; 21.9%). The H-89 inhibitor used at a concentration of 10−9 M (4 of 29; 13.8%) decreased (P < 0.05) the number of oocytes reaching nuclear maturation when compared with eGH (11 of 29; 38%). The DDA inhibitor at a concentration of 10−8 M (2 of 27; 7.4%) also reduced (P < 0.05) the number of oocytes reaching maturity when compared with the eGH group (9 of 30; 30%). Results from the present study show that H-89 and DDA can be used in vitro to block the eGH effect on equine oocyte maturation.

Keywords

Equine growth hormoneInhibitorsMeiosis resumptionOocyte maturation
Type
Research Article
Information
Zygote , Volume 23 , Issue 6 , December 2015 , pp. 795 - 801
Copyright
Copyright © Cambridge University Press 2014 

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