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Specific activation of the hb4 gene in the Xenopus oocyte through a Nobox-binding element located at the proximal promoter sequence

Published online by Cambridge University Press:  28 June 2019

Masanori Nakamigawa ,
Takumi Kondo  and
Mitsugu Maéno Open the ORCID record for Mitsugu Maéno [Opens in a new window]
Masanori Nakamigawa
Affiliation:
Graduate School of Science and Technology;
Takumi Kondo
Affiliation:
Graduate School of Science and Technology;
Mitsugu Maéno
Affiliation:
Institute of Science and Technology, Niigata University, 8050 Ikarashi-2, Nishi-ku, Niigata 950-2181, Japan
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Summary

We isolated and characterized Xenopus tropicalis hb4 flanking DNA and showed that the −3076/+29 sequence was able to drive stage-specific transcription in the developmental process. Transgenic reporter analysis indicated that green fluorescent protein was expressed in the ovaries of female frogs at 3 months of age and in both the ovaries and testis of frogs at 6 months of age. A series of experiments with deletion of the flanking sequence and a subsequent luciferase reporter assay revealed that there were two positive regulatory regions and that the most proximal sequence of the promoter region had a certain level of transcriptional activity in oocytes. Subsequently, we showed that a conserved sequence containing Nobox-binding element (NBE) was essential for transcriptional activation and that Nobox expressed in the ovary had a crucial role in hb4 transcription through the NBE sequence.

Keywords

Linker histoneLuciferaseMaternal geneTransgenic frogTranscription
Type
Research Article
Information
Zygote , Volume 27 , Issue 4 , August 2019 , pp. 195 - 202
Copyright
© Cambridge University Press 2019 

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