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Somatic cells derived from haploid larvae are feasible as donors for nuclear transplant in zebrafish. Preliminary results

Published online by Cambridge University Press:  24 March 2011

J. Cardona-Costa ,
M. Pérez-Camps  and
F. García-Ximénez
J. Cardona-Costa*
Affiliation:
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14, 46022 Valencia, Spain.
M. Pérez-Camps
Affiliation:
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14, 46022 Valencia, Spain.
F. García-Ximénez
Affiliation:
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14, 46022 Valencia, Spain.
*
All correspondence to: J. Cardona-Costa. Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14, 46022 Valencia, Spain. Tel: +34 96 3879433. Fax: +34 96 3877439. e-mail: [email protected]
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Summary

Somatic cells derived from zebrafish haploid larval (both androgenetic and gynogenetic) cultures were used as donors for nuclear transplant into non-enucleated oocytes. Nuclei were transplanted either before or simultaneously with oocyte activation in the central region and in the incipient animal pole, respectively. Against expected results, 20% of transplanted embryos during oocyte activation using cells of gynogenetic origin reached the 100% epiboly stage, even two survived for up to 5 days, whereas no development was observed when cells from androgenetic origin were used. Results derived from this work open a novel possibility of studying somatic cell reprogramming and imprinting phenomena in zebrafish.

Keywords

Cell cultureHaploidNuclear transplantVertebrateZebrafish
Type
Research Article
Information
Zygote , Volume 20 , Issue 3 , August 2012 , pp. 277 - 280
Copyright
Copyright © Cambridge University Press 2011

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