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Sodium butyrate improves the cloned yak embryo viability and corrects gene expression patterns

Published online by Cambridge University Press:  12 June 2013

Xian-rong Xiong ,
Dao-liang Lan ,
Jian Li ,
Yong Wang  and
Jin-cheng Zhong
Xian-rong Xiong
Affiliation:
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, China.
Dao-liang Lan
Affiliation:
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, China.
Jian Li*
Affiliation:
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, PR China.
Yong Wang
Affiliation:
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, China.
Jin-cheng Zhong
Affiliation:
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, China.
*
All correspondence to: Jian Li. College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, PR China. Tel: +86 028 85528277. e-mail: [email protected]
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Summary

Interspecies somatic cell nuclear transfer (iSCNT), a powerful tool in basic scientific research, has been used widely to increase and preserve the population of endangered species. Yak (Bos grunniens) is one of these species. Development to term of interspecies cloned yak embryos has not been achieved, possibly due to abnormal epigenetic reprogramming. Previous studies have demonstrated that treatment of intraspecies cloned embryos with (NaBu) significantly improves nuclear–cytoplasmic reprogramming and viability in vitro. Therefore, in this study, we evaluated the effect of optimal NaBu concentration and exposure time on preimplantation development of yak iSCNT embryos and on the expression patterns of developmentally important genes. The results showed that 8-cell rate, blastocyst formation rate and total cell number increased significantly compared with their untreated counterparts when yak iSCNT embryos were treated with 5 nM NaBu for 12 h after activation, but that the 2-cell stage embryo rate was not significantly different. The treatment of NaBu also increased significantly the expression levels of Oct-4 and decreased the expression levels of HDAC-2, Dnmt-1 and IGF-1; the expression patterns of these genes were more similar to that of their bovine–yak in vitro fertilization (BY-IVF) counterparts. The results described above indicated that NaBu treatment improved developmental competence in vitro and ‘corrected’ the gene expression patterns of yak iSCNT embryos.

Keywords

DevelopmentExpressioniSCNTSodium butyrateYak
Type
Research Article
Information
Zygote , Volume 23 , Issue 1 , February 2015 , pp. 19 - 26
Copyright
Copyright © Cambridge University Press 2013 

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References

Beebe, L.F.S., Mcllfatrick, S.J. & Nottle, M.B. (2009). Cytochalasin B and trichostatin A treatment postactivation improves in vitro development of porcine somatic cell nuclear transfer embryos. Cloning Stem Cells 11, 477–82.CrossRefGoogle ScholarPubMed
Bosak, N., Fujisaki, S., Kiuchi, S., Hiraiwa, H. & Yasue, H. (2003). Assignment of DNA cytosine-5-methyltransferase 1 (DNMT1) gene to porcine chromosome 2q21–q22 by somatic cell and radiation hybrid panel mapping. Cytogenet. Genome Res. 101, 178.Google Scholar
Bui, H.T., Wakayama, S., Kishigami, S., Park, K.K., Kim, J.H., Thuan, N.V. & Wakayama, T. (2010). Effect of trichostatin A on chromatin remodeling, histone modifications, DNA replication, and transcriptional activity in cloned mouse embryos. Biol. Reprod. 83, 454–63.CrossRefGoogle ScholarPubMed
Costa-Borges, N., Santalo, J. & Ibanez, E. (2010). Comparison between the effects of valproic acid and trichostatin A on the in vitro development, blastocyst quality, and full-term development of mouse somatic cell nuclear transfer embryos. Cell. Reprogram. 12, 437–46.CrossRefGoogle ScholarPubMed
Couldrey, C. & Lee, R. (2010). DNA methylation patterns in tissues from mid-gestation bovine fetuses produced by somatic cell nuclear transfer show subtle abnormalities in nuclear reprogramming. BMC Dev. Biol. 10, 27.CrossRefGoogle ScholarPubMed
Das, Z.C., Gupta, M.K., Uhm, S.J. & Lee, H.T. (2010). Increasing histone acetylation of cloned embryos, but not donor cells, by sodium butyrate improves their in vitro development in pigs. Cell. Reprogram. 12, 95–104.CrossRefGoogle Scholar
Gómez, M.C., Pope, C.E., Kutner, R.H., Ricks, D.M., Lyons, L.A., Ruhe, M., Dumas, C., Lyons, J., López, M., Dresser, B.L. & Reiser, J. (2008). Nuclear transfer of sand cat cells into enucleated domestic cat oocytes is affected by cryopreservation of donor cells. Cloning Stem Cells 10, 469–84.CrossRefGoogle ScholarPubMed
Kishigami, S., Mizutani, E., Ohta, H., Takafusa, H., Thuan, N.V., Wakayama, S., Bui, H.T. & Wakayama, T. (2006). Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer. Biochem. Biophys. Res. Commun. 340, 183–9.CrossRefGoogle ScholarPubMed
Lager, A.E., Ragina, N.P., Ross, P.J., Beyhan, Z., Cunniff, K., Rodriguez, R.M. & Cibelli, J.B. (2008). Trichostatin A improves histone acetylation in bovine somatic cell nuclear transfer early embryos. Cloning Stem Cells 10, 371–80.Google Scholar
Lee, H.S., Yu, X.F., Bang, J.I., Cho, S.J., Gautam, K.D., Kim, B.W. & Kong, I.K. (2010). Enhanced histone acetylation in somatic cells induced by a histone deacetylases inhibitor improved inter-generic cloned leopard cat blastocysts. Theriogenology 74, 1439–49.CrossRefGoogle Scholar
Li, X., Kato, Y., Tsuji, Y. & Tsunoda, Y. (2008). The effects of trichostatin A on mRNA expression of chromatin structure-, DNA methylation-, and development-related genes in cloned mouse blastocysts. Cloning Stem Cells 10, 133–42.CrossRefGoogle ScholarPubMed
Loi, P., Ptak, G., Barboni, B., Fulka, J., Cappai, P. & Clinton, M. (2001). Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. Nat. Biotechnol. 19, 962–4.CrossRefGoogle ScholarPubMed
Maalouf, W.E., Liu, Z.C., Brochard, V., Renard, J.P., Debey, P., Beaujean, N. & Zink, D. (2009). Trichostatin A treatment of cloned mouse embryos improves constitutive heterochromatin remodeling as well as developmental potential to term. BMB Dev. Biol. 9, 11.CrossRefGoogle ScholarPubMed
Martha, C., Gomez, C., Earle, P, Monica, N., Biancardi, C.D., Galiguis, J., Morris, A.C., Wang, G.S. & Dresser, B.L. (2011). Trichostatin A modified histone covalent pattern and enhanced expression of pluripotent genes in interspecies black-footed cat cloned embryos but did not improve in vitro and in vivo viability. Cell. Reprogram. 13, 315–29.Google Scholar
Miyamoto, K., Furusawa, T., Ohnuki, M., Goel, S., Tokunaga, T., Minami, N., Yamada, M., Ohsumi, K. & Imai, H. (2007). Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts. Mol. Reprod. Dev. 74, 1268–77.CrossRefGoogle ScholarPubMed
Mohana, K.B., Song, H.J., Cho, S.K., Balasubramanian, S., Choe, S.Y. & Rho, G.J. (2007). Effect of histone acetylation modification with sodium butyrate, a histone deacetylase inhibitor, on cell cycle, apoptosis, ploidy and gene expression in porcine fetal fibroblasts.J. Reprod. Dev. 53, 903–13.CrossRefGoogle Scholar
Murko, C., Lagger, S., Steiner, M., Seiser, C., Schoefer, C. & Pusch, O. (2010). Expression of class I histone deacetylases during chick and mouse development. Int. J. Dev. Biol. 54, 1527–37.CrossRefGoogle ScholarPubMed
Ning, S.F., Li, Q.Y., Liang, M.M., Yang, X.G., Xu, H.Y., Lu, Y.Q., Lu, S.S. & Lu, K.H. (2012). Methylation characteristics and developmental potential of Guangxi Bama minipig (Sus scrofa domestica) cloned embryos from donor cells treated with trichostatin A and 5-aza-2′-deoxycytidine. Zygote 22, 1–9.Google Scholar
Oh, H.J., Kim, M.K., Jang, G., Kim, H.J., Hong, S.G., Park, J.E., Park, K., Park, C., Sohn, S.H., Kimb, D.Y., Shin, N.S. & Lee, B.C. (2008). Cloning endangered gray wolves (Canis lupus) from somatic cells collected postmortem. Theriogenology 70, 638–47.CrossRefGoogle ScholarPubMed
Okano, M., Bell, D.W., Haber, D.A. & Li, E. (1999). DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99, 247–57.CrossRefGoogle Scholar
Shao, G.B., Ding, H.M., Gao, W.L., Ding, H.M., Gao, W.L., Li, S.H., Wu, C.F., Xu, Y.X. & Liu, H.L. (2009). Effect of trichostatin A treatment on gene expression in cloned mouse embryos. Theriogenology 71, 1245–52.CrossRefGoogle ScholarPubMed
Shi, W., Hoeflich, A., Flaswinkel, H., Stojkovic, M., Wolf, E. & Zakhartchenko, V. (2003). Induction of a senescent-like phenotype does not confer the ability of bovine immortal cells to support the development of nuclear transfer embryos. Biol. Reprod. 69, 301–9.CrossRefGoogle Scholar
Shi, L.H., Miao, Y.L., Ouyang, Y.C., Huang, J.C., Lei, Z.L., Yang, J.W., Han, Z.M., Song, X.F., Sun, Q.Y. & Chen, D.Y. (2008). Trichostatin A (TSA) improves the development of rabbit–rabbit intraspecies cloned embryos, but not rabbit–human interspecies cloned embryos. Dev. Dyn. 237, 640–8.CrossRefGoogle Scholar
Srirattana, K., Laowtammathron, C., Devahudi, R., Imsoonthornruksa, S., Sangmalee, A., Tunwattana, W., Lorthongpanich, C., Sripunya, N., Keawmungkun, K., Phewsoi, W., Ketudat-Cairns, M. & Parnpai, R. (2008). Effect of trichostatin A on developmental potential of interspecies cloned gaur (Bos gaurus) embryos. Reprod. Fertil. Dev. 21, 126.CrossRefGoogle Scholar
Velazquez, M.A., Zaraza, J., Oropeza, A., Webb, R. & Niemann, H. (2009). The role of IGF1 in the in vivo production of bovine embryos from superovulated donors. Reproduction 137, 161–80.CrossRefGoogle ScholarPubMed
Vogel, G. (2001). Endangered species cloned gaur a short-lived success. Science 291, 409.CrossRefGoogle Scholar
Wang, L.J., Zhang, H., Wang, Y.S., Xu, W.B., Xiong, X.R., Li, Y.Y., Su, J.M., Hua, S. & Zhang, Y. (2011). Scriptaid improves in vitro development and nuclear reprogramming of somatic cell nuclear transfer bovine embryos. Cell. Reprogram. 13, 431–9.CrossRefGoogle ScholarPubMed
Xiong, X.R., Wang, L.J., Zi, X.D., Ma, L., Xu, W.B., Wang, Y.S. & Li, J. (2012). Epigenetic reprogramming of yak iSCNT embryos after donor cell pre-treatment with oocyte extracts. Anim. Reprod. Sci. 133, 229–36.CrossRefGoogle ScholarPubMed
Xu, J. & Yang, X.Z. (2001). Telomerase activity in early bovine embryos derived from parthenogenetic activation and nuclear transfer. Biol. Reprod. 64, 3770–4.CrossRefGoogle ScholarPubMed
Zhao, J., Hao, Y., Ross, J.W, Spate, E.M., Walters, M.S., Samuel, A.R., Clifton, N. M. & Randall, S.P. (2010). Histone deacetylases inhibitors improve in vitro and in vivo developmental competence of somatic cell nuclear transfer porcine embryos. Cell. Reprogram. 12, 75–83.CrossRefGoogle ScholarPubMed