Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-26T11:17:21.818Z Has data issue: false hasContentIssue false
  • English
  • Français

Replacement of glutamine with the dipeptide derivative alanyl-glutamine enhances in vitro maturation of porcine oocytes and development of embryos

Published online by Cambridge University Press:  27 February 2013

Su Jin Kim ,
Ok Jae Koo ,
Dae Kee Kwon ,
Jung Taek Kang ,
Sol Ji Park ,
Ma Ninia Gomez ,
Mohammad Atikuzzaman ,
Goo Jang  and
Byeong-Chun Lee
Su Jin Kim
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Ok Jae Koo
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Dae Kee Kwon
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Jung Taek Kang
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Sol Ji Park
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Ma Ninia Gomez
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Mohammad Atikuzzaman
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Goo Jang
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Byeong-Chun Lee*
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
*
All correspondence to Byeong-Chun Lee. Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea. Tel: +82 2 880 1269. Fax: +82 2 873 1269. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

The presence of glutamine (Gln) in in vitro maturation (IVM) and in vitro culture (IVC) medium is a more potent factor for improving porcine oocyte and embryo development than other amino acids. However Gln is inherently unstable and spontaneously breaks down into ammonia, and therefore interferes with proper development. To avoid this adverse effect, Gln was replaced in the present study with its stable dipeptide derivative alanyl-glutamine (Ala-Gln) and the effects of this replacement on porcine IVM and IVC were evaluated. Replacement of Gln with Ala-Gln during IVM did not improve nuclear maturation, however numbers of early cleaved embryos were significantly increased after activation. Blastocyst formation rates were also significantly improved by using Ala-Gln during IVM. Replacement of Gln with Ala-Gln during IVC significantly increased total cell numbers in blastocysts. Blastocyst formation rate was also significantly higher when Ala-Gln was used in both IVM and IVC. In conclusion, the use of Ala-Gln rather than Gln gives better results for development in both porcine IVM and IVC.

Keywords

Alanyl-glutamineGlutaMAXGlutamineIn vitro maturationPorcine embryo culture
Type
Short Communications
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 286 - 289
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Biggers, J.D., McGinnis, L.K. & Lawitts, J.A. (2004). Enhanced effect of glycyl-l-glutamine on mouse preimplantation embryos in vitro. Reprod. Biomed. Online 9, 59–69.Google Scholar
Brison, D.R. & Schultz, R.M. (1997). Apoptosis during mouse blastocyst formation: evidence for a role for survival factors including transforming growth factor alpha. Biol. Reprod. 56, 1088–96.CrossRefGoogle ScholarPubMed
Gardner, D.K. & Lane, M. (1993). Amino acids and ammonium regulate mouse embryo development in culture. Biol. Reprod. 48, 377–85.Google Scholar
Hammon, D.S., Wang, S. & Holyoak, G.R. (2000). Ammonia concentration in bovine follicular fluid and its effect during in vitro maturation on subsequent embryo development. Anim. Reprod. Sci. 58, 1–8.CrossRefGoogle ScholarPubMed
Hardy, K. (1997). Cell death in the mammalian blastocyst. Mol. Hum. Reprod. 3, 919–25.Google Scholar
Hong, J. & Lee, E. (2007). Intrafollicular amino acid concentration and the effect of amino acids in a defined maturation medium on porcine oocyte maturation, fertilization, and preimplantation development. Theriogenology 68, 728–35.CrossRefGoogle Scholar
Isom, S. C., Li, R.F., Whitworth, K.M. & Prather, R.S. (2012). Timing of first embryonic cleavage is a positive indicator of the in vitro developmental potential of porcine embryos derived from in vitro fertilization, somatic cell nuclear transfer and parthenogenesis. Mol. Reprod. Dev. 79, 197–207.CrossRefGoogle ScholarPubMed
Koo, O.J., Lee, D.W., Kang, J.T., Kwon, D.K., Park, H.J., Park, S.J., Kim, S.J., Jang, G. & Lee, B.C. (2010). Selection of early cleaved embryos and optimal recipients to improve efficiency of pig cloning. J. Emb. Trans. 25, 221–7.Google Scholar
Lane, M. & Gardner, D.K. (2003). Ammonium induces aberrant blastocyst differentiation, metabolism, pH regulation, gene expression and subsequently alters fetal development in the mouse. Biol. Reprod. 69, 1109–17.Google Scholar
Park, H.J., Koo, O.J., Kwon, D.K., Kang, J.T., Jang, G. & Lee, B.C. (2010). Effect of roscovitine-treated donor cells on development of porcine cloned embryos. Reprod. Domest. Anim. 45, 1082–8.Google Scholar
Petters, R.M., Johnson, B.H., Reed, M.L. & Archibong, A.E. (1990). Glucose, glutamine and inorganic phosphate in early development of the pig embryo in vitro. J. Reprod. Fertil. 89, 269–75.Google Scholar
Roth, E., Ollenschlager, G., Hamilton, G., Simmel, A., Langer, K., Fekl, W. & Jakesz, R. (1988). Influence of two glutamine-containing dipeptides on growth of mammalian cells. In Vitro Cell Dev. Biol. 24, 696–8.Google Scholar
Summers, M.C., McGinnis, L.K., Lawitts, J.A. & Biggers, J.D. (2005). Mouse embryo development following IVF in media containing either l-glutamine or glycyl-l-glutamine. Hum. Reprod. 20, 1364–71.Google Scholar
Suzuki, C., Yoshioka, K., Sakatani, M. & Takahashi, M. (2007). Glutamine and hypotaurine improves intracellular oxidative status and in vitro development of porcine preimplantation embryos. Zygote 15, 317–24.Google Scholar
Swain, J.E., Bormann, C.L., Clark, S.G., Walters, E.M., Wheeler, M.B. & Krisher, R.L. (2002). Use of energy substrates by various stage preimplantation pig embryos produced in vivo and in vitro. Reproduction 123, 253–60.Google Scholar
Vickery, H.B., Pucher, G.W. & Clark, H.E. (1935). The determination of glutamine in the presence of asparagine. Biochem. J. 29, 2710–20.Google Scholar
Yoshioka, K., Suzuki, C., Tanaka, A., Anas, I.M. & Iwamura, S. (2002). Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol. Reprod. 66, 112–9.CrossRefGoogle Scholar
Yu, Y., Ding, C., Wang, E., Chen, X., Li, X., Zhao, C., Fan, Y., Wang, L., Beaujean, N., Zhou, Q., Jouneau, A. & Ji, W. (2007). Piezo-assisted nuclear transfer affects cloning efficiency and may cause apoptosis. Reproduction 133, 947–54.Google Scholar