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Protein kinase inhibitors induce the interphase transition by inactivating mitogen-activated protein kinase in mouse eggs

Published online by Cambridge University Press:  01 August 1998

Qing-Yuan Sun
Affiliation:
Department of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900 Israel Institute of Zoology, Academia Sinica, Beijing, 100080 China
Ayala Luria
Affiliation:
Department of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900 Israel
Sara Rubinstein
Affiliation:
Department of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900 Israel
Haim Breitbart
Affiliation:
Department of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900 Israel

Abstract

The role of mitogen-activated protein (MAP) kinase in mouse egg activation induced by protein kinase inhibitors and a protein tyrosine kinase (PTK) inhibitor was investigated. Separated egg proteins were first probed with anti-Active MAP kinase antibody and then re-probed with anti-ERK2 antibody. Staurosporine and Ro-31-8220, at concentrations that normally inhibit protein kinase C, did not affect egg activation or MAP kinase activity, while higher dosages caused egg activation. Staurosporine at 2 μM induced the metaphase–interphase transition without emission of the second polar body (PB2), while Ro-31-8220 at 40 μM induced PB2 emission, first cleavage, and then the transition to interphase. Half the eggs were also activated by the PTK inhibitor genistein. In each treatment, the proportion of eggs that entered interphase was well correlated with the degree of MAP kinase inactivation. Artificial activation of this kinase by okadaic acid overcame the interphase transition. These data suggest that protein kinase inhibitors and a protein tyrosine kinase inhibitor induce the interphase transition by inactivating MAP kinase in mouse eggs.

Type
Research Article
Copyright
© 1998 Cambridge University Press

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