Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-26T11:14:43.511Z Has data issue: false hasContentIssue false
  • English
  • Français

Possible regulation of Oct60 transcription by a positive feedback loop in Xenopus oocytes

Published online by Cambridge University Press:  28 November 2012

Keisuke Morichika ,
Makoto Sugimoto ,
Katsuki Yasuda  and
Tsutomu Kinoshita
Keisuke Morichika*
Affiliation:
Department of Life Science, School of Science, Rikkyo University, 3–34–1 Nishi-ikebukuro, Toshima, Tokyo 178–8501, Japan.
Makoto Sugimoto
Affiliation:
Department of Life Science, School of Science, Rikkyo University, 3–34–1 Nishi-ikebukuro, Toshima, Tokyo 178–8501, Japan.
Katsuki Yasuda
Affiliation:
Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, 2–1 Gakuen, Sanda, Hyogo, 669–1337, Japan.
Tsutomu Kinoshita
Affiliation:
Department of Life Science, School of Science, Rikkyo University, 3–34–1 Nishi-ikebukuro, Toshima, Tokyo 178–8501, Japan.
*
All correspondence to: Keisuke Morichika. Department of Life Science, School of Science, Rikkyo University, 3–34–1 Nishi-ikebukuro, Toshima, Tokyo 178–8501, Japan. Tel: +81 3 3985 4766. Fax: +81 3 3985 4766. e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

The POU family subclass V (POU-V) proteins have important roles in maintaining cells in an undifferentiated state. In Xenopus, expression of the POU-V protein Oct60 was detected in oocytes and was found to decrease in blastula- to gastrula-stage embryos. In addition, Oct60 overexpression inhibits some signals in early embryogenesis, including Activin/Nodal, BMP, and Wnt signalling. In this report, we analysed mechanisms of Oct60 promoter activation and discovered that Oct60 transcription was activated ectopically in somatic nuclei by oocyte extract treatment. Promoter assays demonstrated that Oct60 transcription was activated in oocytes specifically and that this activation was dependent on an Octamer-Sox binding motif. ChIP assays showed that the Oct60 protein binds the motif. These results suggest that Oct60 transcription is regulated by a positive-feedback loop in Xenopus oocytes.

Keywords

Oct60OogenesisPromoter analysisPOU family class VSelf-regulation
Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 266 - 274
Copyright
Copyright © Cambridge University Press 2012 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Byrne, J.A., Simonsson, S., Western, P.S. & Gurdon, J.B. (2003). Nuclei of adult mammalian somatic cells are directly reprogrammed to Oct-4 stem cell gene expression by amphibian oocytes. Curr. Biol. 13, 1206–13.CrossRefGoogle ScholarPubMed
Cao, Y., Knöchel, S., Donow, C., Miethe, J., Kaufmann, E. & Knöchel, W. (2004). The POU factor Oct-25 regulates the Xvent-2B gene and counteracts terminal differentiation in Xenopus embryos. J. Biol. Chem. 279, 43735–43.Google Scholar
Cao, Y., Siegel, D. & Knöchel, W. (2006). Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation. Mech. Dev. 123, 614–25.Google Scholar
Cao, Y., Siegel, D., Donow, C., Knöchel, S., Yuan, L. & Knöchel, W. (2007). POU-V factors antagonize maternal VegT activity and beta-catenin signaling in Xenopus embryos. EMBO J. 26, 2942–54.Google Scholar
Cao, Y., Siegel, D., Oswald, F. & Knöchel, W. (2008). Oct25 represses transcription of nodal/activin target genes by interaction with signal transducers during Xenopus gastrulation. T.J. Biol. Chem. 283, 34168–77.CrossRefGoogle ScholarPubMed
Chew, J.-L., Loh, Y.-H., Zhang, W., Chen, X., Tam, W.-L., Yeap, L.-S., Li, P., Ang, Y.-S., Lim, B., Robson, P. & Ng, H.-H. (2005). Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells. Mol. Cell. Biol. 25, 6031–46.Google Scholar
Dumont, J.N. (1972). Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals. J. Morphol. 136, 153–79.Google Scholar
Frank, D. & Harland, R.M. (1992). Localized expression of a Xenopus POU gene depends on cell-autonomous transcriptional activation and induction-dependent inactivation. Development 115, 439–48.Google Scholar
Gurdon, J.B. (2006). Nuclear transplantation in Xenopus. Methods Mol. Biol. 325, 1–9.Google Scholar
Hinkley, C.S., Martin, J.F., Leibham, D. & Perry, M. (1992). Sequential expression of multiple POU proteins during amphibian early development. Mol. Cell. Biol. 12, 638–49.Google ScholarPubMed
Ito, M., Katada, T., Miyatani, S. & Kinoshita, T. (2007). XSu(H)2 is an essential factor for gene expression and morphogenesis of the Xenopus gastrula embryo. Int. J. Dev. Biol. 51, 27–36.Google Scholar
Iwafuchi-Doi, M., Yoshida, Y., Onichtchouk, D., Leichsenring, M., Driever, W., Takemoto, T., Uchikawa, M., Kamachi, Y. & Kondoh, H. (2011). The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates. Dev. Biol. 352, 354–66.Google Scholar
Morrison, G.M. & Brickman, J.M. (2006). Conserved roles for Oct4 homologues in maintaining multipotency during early vertebrate development. Development 133, 2011–22.Google Scholar
Nishimoto, M., Fukushima, A., Okuda, A. & Muramatsu, M. (1999). The gene for the embryonic stem cell coactivator UTF1 carries a regulatory element which selectively interacts with a complex composed of Oct-3/4 and Sox-2. Mol. Cell. Biol. 19, 5453–65.Google Scholar
Okamoto, K., Okazawa, H., Okuda, A., Sakai, M., Muramatsu, M. & Hamada, H. (1990). A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. Cell 60, 461–72.Google Scholar
Okumura-Nakanishi, S., Saito, M., Niwa, H. & Ishikawa, F. (2005). Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cells. J. Biol. Chem. 280, 5307–17.CrossRefGoogle ScholarPubMed
Palmieri, S.L., Peter, W., Hess, H. & Schöler, H.R. (1994). Oct-4 transcription factor is differentially expressed in the mouse embryo during establishment of the first two extraembryonic cell lineages involved in implantation. Dev. Biol. 166, 259–67.Google Scholar
Pearse, R.V., Drolet, D.W., Kalla, K.A., Hooshmand, F., Bermingham, J.R. & Rosenfeld, M.G. (1997). Reduced fertility in mice deficient for the POU protein sperm-1. Proc. Natl. Acad. Sci. USA 94, 7555–60.CrossRefGoogle ScholarPubMed
Phillips, J.E. & Corces, V.G. (2009). CTCF: master weaver of the genome. Cell 137, 1194–211.Google Scholar
Reim, G. & Brand, M. (2006). Maternal control of vertebrate dorsoventral axis formation and epiboly by the POU domain protein Spg/Pou2/Oct4. Development 133, 2757–70.CrossRefGoogle ScholarPubMed
Reim, G., Mizoguchi, T., Stainier, D.Y., Kikuchi, Y. & Brand, M. (2004). The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein Casanova. Dev. Cell 6, 91–101.Google Scholar
Rodda, D.J., Chew, J.-L., Lim, L.-H., Loh, Y.-H., Wang, B., Ng, H.-H. & Robson, P. (2005). Transcriptional regulation of nanog by OCT4 and SOX2. J. Biol. Chem. 280, 24731–7.Google Scholar
Rosner, M.H., Vigano, M.A., Ozato, K., Timmons, P.M., Poirier, F., Rigby, P.W. & Staudt, L.M. (1990). A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo. Nature 345, 686–92.Google Scholar
Ryan, A.K. & Rosenfeld, M.G. (1997). POU domain family values: flexibility, partnerships, and developmental codes. Genes Dev. 11, 1207–25.Google Scholar
Simonsson, S. & Gurdon, J. (2004). DNA demethylation is necessary for the epigenetic reprogramming of somatic cell nuclei. Nat Cell Biol. 10, 984–90.Google Scholar
Stewart, D., Tomita, A., Shi, Y.-B. & Wong, J. (2006). Chromatin immunoprecipitation for studying transcriptional regulation in Xenopus oocytes and tadpoles. Methods Mol. Biol. 322, 165–81.Google Scholar
Tokuzawa, Y., Kaiho, E., Maruyama, M., Takahashi, K., Mitsui, K., Maeda, M., Niwa, H. & Yamanaka, S. (2003). Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. Mol. Cell. Biol. 23, 2699–708.Google Scholar
Tomioka, M., Nishimoto, M., Miyagi, S., Katayanagi, T., Fukui, N., Niwa, H., Muramatsu, M. & Okuda, A. (2002). Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex. Nucleic Acids Res. 30, 3202–13.Google Scholar
Whitfield, T., Heasman, J. & Wylie, C. (1993). XLPOU-60, a Xenopus POU-domain mRNA, is oocyte-specific from very early stages of oogenesis, and localised to presumptive mesoderm and ectoderm in the blastula. Dev. Boil. 155, 361–70.Google Scholar
Yuan, H., Corbi, N., Basilico, C. & Dailey, L. (1995). Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3. Genes Dev. 9, 2635–45.Google Scholar