Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-23T15:35:21.412Z Has data issue: false hasContentIssue false
  • English
  • Français

Peijingsu effectively improves sperm DNA integrity

Published online by Cambridge University Press:  22 February 2021

Caixia Yuan ,
Haixia Song ,
Zhulin Wang  and
Huaixiu Wang Open the ORCID record for Huaixiu Wang [Opens in a new window]
Caixia Yuan
Affiliation:
Department of Reproductive Medicine, Shanxi Provincial Hospital, Taiyuan, China
Haixia Song
Affiliation:
Department of Reproductive Medicine, Shanxi Provincial Hospital, Taiyuan, China
Zhulin Wang
Affiliation:
School of Environment and Engineering, Tianjin Industrial University, Tianjin, China
Huaixiu Wang*
Affiliation:
Beijing Perfect Family Hospital, Beijing, China
*
Author for correspondence: Huaixiu Wang. Beijing Perfect Family Hospital, Beijing, 100034, China. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Intact human sperm DNA is an essential prerequisite for successful fertilization and embryo development. Abnormal sperm DNA fragmentation is a independent factor for male infertility. The objective of this study was to investigate the effects of Peijingsu, a health product, on the DNA integrity of human sperm. Peijingsu was administered for 15 days to 22 patients who had an abnormal sperm DNA fragmentation index (DFI). The DFIs before and after treatment were compared and analyzed using paired t-test. DFIs decreased significantly (P = 0.0008) after treatment, therefore it was concluded that Peijingsu effectively improved sperm DNA integrity in infertile patients who had an abnormal sperm DFI.

Keywords

DNA fragmentation indexHealth productMale infertilityPeijingsuSperm
Type
Research Article
Information
Zygote , Volume 29 , Issue 4 , August 2021 , pp. 260 - 263
Copyright
© The Author(s), 2021. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

These authors contributed equally to this work.

References

Abad, C, Amengual, MJ, Gosalve, ZJ, Coward, K, Hannaoui, N, Benet, J, Garcia-Peiro, A and Prats, J (2013). Effects of oral antioxidant treatment upon the dynamics of human sperm DNA fragmentation and subpopulation of sperm with highly degraded DNA. Andrologia 45, 211–6.CrossRefGoogle ScholarPubMed
Agarwal, A and Allameneni, SSR (2004). The effect of sperm DNA damage in assisted reproduction outcomes. Minerva Ginecol 56, 23–45.Google ScholarPubMed
Agarwal, A, Nallela, KP, Allamaneni, SS and Said, TM (2004). Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online 8, 616–27.CrossRefGoogle ScholarPubMed
Agarwal, A and Said, TM (2003). Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update 9, 331–45.CrossRefGoogle ScholarPubMed
Agarwal, A, Saleh, RA and Bedaiwy, MA (2003). Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 79, 829–43.CrossRefGoogle ScholarPubMed
Aitken, RJ and De Iuliis, GN (2007). Value of DNA integrity assays for fertility evaluation. Soc Reprod Fertil 65(Suppl), 81–92.Google ScholarPubMed
Aitken, RJ and Krausz, C (2001). Oxidative stress, DNA damage and the Y chromosome. Reproduction 122, 497–506.CrossRefGoogle ScholarPubMed
Aoki, VW, Liu, L and Carrell, DT (2005). Identification and evaluation of a novel sperm protamine abnormality in a population of infertile males. Hum Reprod 20, 1298–306.CrossRefGoogle Scholar
Bedford, JM and Calvin, HI (1974). The occurrence and possible functional significance of –S-S cross links in sperm heads, with particular reference to eutherian mammals. J Exp Zool 188, 137–55.CrossRefGoogle Scholar
Benchaib, M, Lornage, J, Mazoyer, C, Lejeune, H, Salle, B and Francois, GJ (2007) Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome. Fertil Steril 87, 93–100.CrossRefGoogle ScholarPubMed
Cline, SD and Hanawalt, PC (2003).Who’s on first in the cellular response to DNA damage? Nat Rev Mol Cell Biol 4, 361–72.CrossRefGoogle ScholarPubMed
Collins, JA, Barnhart, KJ and Schlegel, PN (2008). Do sperm DNA integrity tests predict pregnancy with in vitro fertilization? Fertil Steril 89, 823–31.CrossRefGoogle ScholarPubMed
Donnelly, ET, McClure, N and Lewis, SE (1999).The effect of ascorbate and alpha-tocopherol supplementation in vitro on DNA integrity and hydrogen peroxide-induced DNA damage in human spermatozoa. Mutagenesis 23, 25–43.Google Scholar
Duran, EH, Morshedi, M, Taylor, S and Oehninger, S (2002). Sperm DNA quality predicts intrauterine insemination outcome: a prospective cohort study. Hum Reprod 17, 3122–8.CrossRefGoogle ScholarPubMed
Duru, NK, Morshedi, M and Oehninger, S (2001). Effects of H2O2 on DNA and plasma membrane integrity of human spermatozoa. Fertil Steril 82, 1200–7.Google Scholar
Evenson, DP, Larson, KL and Jost, LK (2002). Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparison with other techniques. J Androl 23, 25–43.CrossRefGoogle Scholar
Frazer, L (2004). Structural damage to nuclear DNA in mammalian spermatozoa: its evaluation technique and relationship with male infertility. Pol J Vet Sci 7, 311–21.Google Scholar
Ghyasvand, T, Goodarzi, MT, Amiri l Karimi J and Ghorbani M (2015). Serum levels of lycopene, beta-carotene and retinol and their correlation with sperm DNA damage in normospermic and infertile men. Int J Reprod Biomed 13, 787–92.Google ScholarPubMed
Greco, E, Scarselli, F, Iacobelli, M, Rienzi, L, Ubaldi, F, Ferrero, S, Franco, G, Anniballo, N, Mendoza, C and Tesarik, J (2005). Efficient treatment of infertility due to sperm DNA damage by ICSI with testicular spermatozoa. Hum Reprod 20, 226–30.CrossRefGoogle ScholarPubMed
Gunes, S, Al-Sadean, M and Agarwal, A (2015). Spermatogenesis, DNA damage and DNA repair mechanisms in male infertility. Reprod Biomed Online 31, 309–19.CrossRefGoogle ScholarPubMed
Henkel, R, Hajimohammed, M, Stalf, T, Hoogendijk, C, Mehnert, C, Menkveld, R, Gips, H, Schill, WB and Kruger, TF (2004). Influence of deoxyribonucleic acid damage on fertilization and pregnancy. Fertil Steril 81, 965–72.CrossRefGoogle Scholar
Hoeijmakers, JHJ (2001). Genome maintenance mechanism for preventing cancer. Nature 411, 366–74.CrossRefGoogle Scholar
Imamovic, KS and Pinter, B (2014). Review of clinical trials on effects of oral antioxidants on basic semen and other parameters in idiopathic oligoasthenoteratozoospermia. Biomed Res Int 2014, 426951.Google Scholar
Iman, SN, Shamsi, MB, Kumar, K, Deka, D and Dada, R (2011). Idiopathic recurrent pregnancy loss: role of paternal factors; a pilot study. J Reprod Infertil 12, 267–76.Google Scholar
Kamkar, N, Ramezanali, F and Sabbaghian, M (2018).The relationship between sperm DNA fragmentation, free radicals and antioxidant capacity with idiopathic repeated pregnancy loss. Reprod Biol 18, 330–5.CrossRefGoogle ScholarPubMed
Liu, D and Baker, H (1992). Sperm nuclear chromatin normality: relationship with sperm morphology, sperm-zona pellucida binding and fertilization rates in vitro . Fertil Steril 58, 1178–84.CrossRefGoogle ScholarPubMed
Lombardo, F, Sansone, A, Romanelli, F, Paoli, D, Gandini, L and Lenzi, A (2011). The role of antioxidant therapy in the treatment of male infertility: an overview. Asian J Androl 13, 690–7.CrossRefGoogle ScholarPubMed
Lopes, S, Jurisicova, A, Sun, JG and Casper, RF (1998). Reactive oxygen species : potential cause for DNA fragmentation in human spermatozoa. Hum Reprod 13, 896–900.CrossRefGoogle ScholarPubMed
Marcon, L and Boissonneault, G (2004). Transit DNA strand breaks during mouse and human spermiogenesis: new insights in stage specificity and link to chromatin remodeling. Biol Reprod 70, 910–8.CrossRefGoogle Scholar
Menezo, YJR, Hazout, A, Panteix, G, Robert, F, Rollet, J, Cohen-Bacrie, P, Chapuis, F, Clement, P and Benkhalifa, M (2007). Antioxidants to reduce sperm DNA fragmentation : an unexpected adverse effect. Reprod Biomed Online 14, 418–21.CrossRefGoogle Scholar
Morris, ID, Ilott, S, Dixon, L and Brison, DR (2002). The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (comet assay) and its relationship to fertilization and embryo development. Hum Reprod 17, 990–8.CrossRefGoogle ScholarPubMed
Moustafa, MH, Sharma, RK, Thornton, J, Mascha, E, Abdel-Hafez, MA, Thomas, AJ Jr Agarwal, A (2004). Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Hum Reprod 19, 129–38.CrossRefGoogle ScholarPubMed
Padron, OF, Brachett, NL, Sharma, RK, Lynne, CM, Thomas, AJ and Agarwal, A (1997). Seminal reactive oxygen species, sperm motility and morphology in men with spinal cord injury. Fertil Steril 67, 1115–20.CrossRefGoogle ScholarPubMed
Peris, SI, Bilodeau, J, Dufour, M and Bailey, JL (2007). Impact of cryopreservation and reactive oxygen species on DNA integrity, lipid peroxidation and functional parameters in ram sperm. Mol Reprod Dev 74, 878–92.CrossRefGoogle ScholarPubMed
Sailer, BL, Jost, LK and Evenson, DP (1995). Mammalian sperm DNA susceptibility to in-situ denaturation associated with the presence of DNA strand breaks as measured by the terminal deoxynucleotidyl transferase assay. J Androl 16, 80–7.Google ScholarPubMed
Sakkas, D and Alvarez, JG (2010). Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome and analysis. Fertil Steril 93, 1027–36.CrossRefGoogle ScholarPubMed
Sakkas, D, Manicardi, G, Bianchi, PG, Bizzaro, D and Bianchi, U (1995). Relationship between the presence of endogenous nicks and sperm chromatin packaging in maturing and fertilizing mouse spermatozoa. Biol Reprod 52, 1149–55.CrossRefGoogle ScholarPubMed
Sakkas, D, Mariethoz, E and St John, JC (1999). Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the Fas-Mediated pathway. Exp Cell Res 251, 350–5.CrossRefGoogle ScholarPubMed
Saleh, RA and Agarwal, A (2002). Oxidative stress and male infertility: from research bench to clinical practice. J Androl 23, 737–52.Google ScholarPubMed
Saleh, RA, Agarwal, A, Sharma, RK, Said, TM, Sikka, SC and Thomas, AJ Jr (2003). Evaluation of nuclear DNA damage in spermatozoa from infertile men with varicocele. Fertil Steril 80, 1431–6.CrossRefGoogle ScholarPubMed
Selvam, MKP and Agarwal, A (2018). A systematic review on sperm DNA fragmentation in male factor infertility: laboratory assessment. Arab J Urol 16, 65–76.CrossRefGoogle Scholar
Shaha, C, Tripathi, R and Mishra, DP (2010). Male germ cell apoptosis: regulation and biology. Philos Trans R Soc Lond B Biol Sci 365, 1501–15.CrossRefGoogle ScholarPubMed
Shansi, MB, Kumar, R and Dade, R (2008). Evaluation of nuclear DNA damage in human spermatozoa in men opting for assisted reproduction. Indian J Med Res 127, 115–23.Google Scholar
Simon, L, Murphy, K, Shamsi, MB, Liu, L, Emery, B, Aston, KI, Hotaling, J and Carrell, DT (2014). Paternal influence of sperm DNA integrity on early embryonic development. Hum Reprod 29, 2402–12.CrossRefGoogle ScholarPubMed
Steele, EK, McClure, N, Maxwell, RJ and Lewis, SE (1999). A comparison of DNA damage in testicular and proximal epididymal spermatozoa in obstructive azoospermia. Mol Hum Reprod 5, 831–5.CrossRefGoogle ScholarPubMed
Tvrda, E, Kovacik, A, Tusiimova, E, Paai, D, Mackovich, A, Alimov, J and Lukac, N (2016). Antioxidant efficiency of lycopene on oxidative stress-induced damage in bovine spermatozoa. J Anim Sci Biotechnol 7, 50.CrossRefGoogle ScholarPubMed
Twigg, J, Fulton, N and Gomez, E (1998). Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: lipid peroxidation, DNA fragmentation and the effectiveness of antioxidants. Hum Reprod 13, 1429–36.CrossRefGoogle ScholarPubMed
Walczak-Jedrzejowska, R, Wolski, JK and Slowikowska-Hilczer, J (2013).The role of oxidative stress and antioxidants in male fertility. Cent European J Urol 66, 60–7.CrossRefGoogle ScholarPubMed
Ward, WS and Coffey, DS (1991). DNA packaging and organization in mammalian spermatozoa: comparison with somatic cells. Biol Reprod 44, 569–74.CrossRefGoogle ScholarPubMed
Zini, A, Gabriel, MS and Libman, J (2010). Lycopene supplementation in vitro can protect human sperm deoxyribonucleic acid from oxidative damage. Fertil Steril 94, 1033–6.CrossRefGoogle ScholarPubMed
Zini, A, Kamal, K, Phang, D, Willis, J and Jarvi, K (2001). Biologic variability of sperm DNA denaturation in infertile men. Urology 58, 258–61.CrossRefGoogle ScholarPubMed