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Nuclear Sm antigens in the sperm of different organisms

Published online by Cambridge University Press:  26 September 2008

Fernando Delgadoa ,
Monica Brito ,
Ilona I. Concha ,
Ricardo Schroeder  and
Luis O. Burzio
Fernando Delgadoa
Affiliation:
Instituto de Bioquímica, Facultad de Ciencias Universidad Austral de Chile, Valdivia, Chile.
Monica Brito
Affiliation:
Instituto de Bioquímica, Facultad de Ciencias Universidad Austral de Chile, Valdivia, Chile.
Ilona I. Concha
Affiliation:
Instituto de Bioquímica, Facultad de Ciencias Universidad Austral de Chile, Valdivia, Chile.
Ricardo Schroeder
Affiliation:
Instituto de Bioquímica, Facultad de Ciencias Universidad Austral de Chile, Valdivia, Chile.
Luis O. Burzio*
Affiliation:
Instituto de Bioquímica, Facultad de Ciencias Universidad Austral de Chile, Valdivia, Chile.
*
Luis O.Burzio, Instituto de Bioquímica, Facultad de Ciencias, Universidad Austral de Chile, PO Box 567, Valdiva, Chile. Fax: 0056-63-219410.
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Abstract

Summary

Immunoblot analysis of sperm protein from several species revealed the presence of polypeptides recognised by anti-Sm sera obtained from patients with systemic lupus erythematosus. Immunoreactive polypeptides in human, bull, mouse and rat sperm were identified as protein B', B and D as compared with the Sm polypeptides of HeLa cells. In the sperm of rooster, the teleost fish Cyprinus carpio and the mussel Choromytilus chorus, the immunoreactive polypeptide profile was more complex. To ascertain the sperm origin of the Sm antigens, immunolocalisation with anti-Sm serum was carried out. The results demonstrated that in all the specied studied staining was confinde to the sperm nucleus, confirming that some polypeptides of the small nuclear ribonnucleoprotein complex are present in the gamete.

Keywords

Anti-Sm serumSm antigenssnRNPsSperm antigens
Type
Article
Information
Zygote , Volume 2 , Issue 3 , August 1994 , pp. 227 - 235
Copyright
Copyright © Cambridge University Press 1994

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