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Nuclear envelope removal/maintenance determines the structural and functional remodelling of embryonic red blood cell nuclei in activated mouse oocytes

Published online by Cambridge University Press:  15 January 2010

Daniel Szöllösi ,
Renata Czołowska ,
Ewa Borsuk ,
Maria S. Szöllösi  and
Pascale Debey
Daniel Szöllösi
Affiliation:
INRA, Jouy-en-Josas, France, University of Warsaw, Poland, IBPC, Paris and Muséum National d'Histoire Naturelle, Paris, France
Renata Czołowska*
Affiliation:
INRA, Jouy-en-Josas, France, University of Warsaw, Poland, IBPC, Paris and Muséum National d'Histoire Naturelle, Paris, France
Ewa Borsuk
Affiliation:
INRA, Jouy-en-Josas, France, University of Warsaw, Poland, IBPC, Paris and Muséum National d'Histoire Naturelle, Paris, France
Maria S. Szöllösi
Affiliation:
INRA, Jouy-en-Josas, France, University of Warsaw, Poland, IBPC, Paris and Muséum National d'Histoire Naturelle, Paris, France
Pascale Debey
Affiliation:
INRA, Jouy-en-Josas, France, University of Warsaw, Poland, IBPC, Paris and Muséum National d'Histoire Naturelle, Paris, France
*
Renata Czołowska, Department of Embryology, University of Warsaw, Krakowskie Przedmiescie 26/28, 00-927 Warsaw 64, Poland. Telephone: +48 22 6 20 03 81 (ext. 475). Fax: +48 22 8 26 86 24. e-mail: [email protected].
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Summary

Nuclei of embryonic red blood cells (e-RBC) from 12-day mouse fetuses are arrested in Go phase of the cell cycle and have low transcriptional activity. These nuclei were transferred with help of polyethylene glycol (PEG)-mediated fusion to parthenogenetically activated mouse oocytes and heterokaryons were analysed for nuclear structure and transcriptional activity. If fusion proceeded 25–45 min after oocyte activation, e-RBC nuclei were induced to nuclear envelope breakdown and partial chromatin condensation, followed by formation of nuclei structurally identical with pronuclei. These ‘pronuclei’, similar to egg (female) pronuclei, remained transcriptionally silent over several hours of in vitro culture. If fusion was performed 1 h or later (up to 7 h) after activation, the nuclear envelope of e-RBC nuclei remained intact and nuclear remodelling was less spectacular (slight chromatin decondensation, formation of nucleolus precursor bodies). These nuclei, however, reinforced polymerase-II-dependent transcription within a few hours of in vitro culture. Our present experiments, together with our previous work, demonstrate that nuclear envelope breakdown/maintenance are critical events for nuclear remodelling in activated mouse oocytes and that somatic dormant nuclei can be stimulated to renew transcription at a time when the female pronucleus remains transcriptionally silent.

Keywords

HeterokaryonsMouse oocyteNuclear remodellingRed blood cell nucleiTranscription
Type
Research Article
Information
Zygote , Volume 6 , Issue 1 , February 1998 , pp. 65 - 73
Copyright
Copyright © Cambridge University Press 1998

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