Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-20T04:04:34.907Z Has data issue: false hasContentIssue false
  • English
  • Français

mInscuteable regulates meiotic spindle organization during mouse oocyte meiotic maturation

Published online by Cambridge University Press:  18 November 2019

Zhuoni Xiao ,
Jiali Peng Open the ORCID record for Jiali Peng [Opens in a new window] ,
Meiting Xie ,
Jing Yang  and
Wangming Xu
Zhuoni Xiao*
Affiliation:
Reproductive Medical Center, Wuhan University Renmin Hospital, Wuhan, China
Jiali Peng
Affiliation:
Reproductive Medical Center, Wuhan University Renmin Hospital, Wuhan, China
Meiting Xie
Affiliation:
Reproductive Medical Center, Wuhan University Renmin Hospital, Wuhan, China
Jing Yang
Affiliation:
Reproductive Medical Center, Wuhan University Renmin Hospital, Wuhan, China
Wangming Xu
Affiliation:
Reproductive Medical Center, Wuhan University Renmin Hospital, Wuhan, China
*
Author for correspondence: Zhuoni Xiao. Reproductive Medical Center, Wuhan University, Renmin Hospital, 238 Jiefang Road, Wuhan, China Tel: +86 18627793566. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Establishment of cellular polarity is one of the key events during oocyte maturation. Inscuteable (Insc) has been identified as a key regulator of cell polarity during asymmetric division in Drosophila. However, the function of its evolutionarily conserved mammalian homologue, mInscuteable (mInsc), in mouse meiotic maturation is not clear. In this study, we investigated the roles of mInsc in mouse oocyte maturation. mInsc was detected at all stages of oocyte maturation. The protein level of mInsc was slightly higher at the germinal vesicle breakdown (GVBD) stage and remained constant during mouse oocyte maturation. The subcellular localization of mInsc overlapped with spindle microtubules. Disruption of microtubules and microfilaments caused changes in the localization of mInsc. Depletion or overexpression of mInsc significantly decreased the maturation rates of mouse oocytes. Depletion of mInsc significantly affected asymmetric division, spindle assembly, alignments of chromosomes and actin cap formation. Taken together, our results demonstrated that mInsc regulates meiotic spindle organization during mouse meiotic maturation.

Keywords

Cell polarityInscuteableMeiosismInscOocyte maturationSpinal organization
Type
Research Article
Information
Zygote , Volume 28 , Issue 1 , February 2020 , pp. 45 - 50
Copyright
© Cambridge University Press 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ai, JS, Wang, Q, Li, M, Shi, LH, Ola, SI, Xiong, B, Yin, S, Chen, DY and Sun, QY (2008) Roles of microtubules and microfilaments in spindle movements during rat oocyte meiosis. J Reprod Dev 54, 391–6.CrossRefGoogle ScholarPubMed
Berdnik, D and Knoblich, JA (2002) Drosophila Aurora-A is required for centrosome maturation and actin-dependent asymmetric protein localization during mitosis. Curr Biol 12, 640–7.CrossRefGoogle ScholarPubMed
Chartier, NT, Hyenne, V and Labbe, JC (2010) [Mechanisms of asymmetric cell division: from model organisms to tumorigenesis]. Med Sci (Paris) 26, 251–7.CrossRefGoogle Scholar
di Pietro, F, Echard, A and Morin, X (2016) Regulation of mitotic spindle orientation: an integrated view. EMBO Rep 17, 1106–30.CrossRefGoogle Scholar
Izaki, T, Kamakura, S, Kohjima, M and Sumimoto, H (2006) Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3. Biochem Biophys Res Commun 341, 1001–6.CrossRefGoogle ScholarPubMed
Jamnongjit, M and Hammes, SR (2005) Oocyte maturation: the coming of age of a germ cell. Semin Reprod Med 23, 234–41.CrossRefGoogle ScholarPubMed
Kloc, M, Ghobrial, RM, Borsuk, E and Kubiak, JZ (2012) Polarity and asymmetry during mouse oogenesis and oocyte maturation. Results Probl Cell Differ 55, 23–44.CrossRefGoogle ScholarPubMed
Knoblich, JA, Jan, LY and Jan, YN (1999) Deletion analysis of the Drosophila Inscuteable protein reveals domains for cortical localization and asymmetric localization. Curr Biol 9, 155–8.CrossRefGoogle ScholarPubMed
Kraut, R and Campos-Ortega, JA (1996) Inscuteable, a neural precursor gene of Drosophila, encodes a candidate for a cytoskeleton adaptor protein. Dev Biol 174, 65–81.CrossRefGoogle ScholarPubMed
Kraut, R, Chia, W, Jan, LY, Jan, YN and Knoblich, JA (1996) Role of inscuteable in orienting asymmetric cell divisions in Drosophila . Nature 383, 50–5.CrossRefGoogle ScholarPubMed
Lechler, T and Fuchs, E (2005) Asymmetric cell divisions promote stratification and differentiation of mammalian skin. Nature 437, 275–80.CrossRefGoogle Scholar
Masui, Y and Clarke, HJ (1979) Oocyte maturation. Int Rev Cytol 57, 185–282.CrossRefGoogle ScholarPubMed
Mauser, JF and Prehoda, KE (2012) Inscuteable regulates the Pins–Mud spindle orientation pathway. PLoS One 7, e29611.CrossRefGoogle ScholarPubMed
Postiglione, MP, Juschke, C, Xie, Y, Haas, GA, Charalambous, C and Knoblich, JA (2011) Mouse inscuteable induces apical–basal spindle orientation to facilitate intermediate progenitor generation in the developing neocortex. Neuron 72, 269–84.CrossRefGoogle ScholarPubMed
Schmittgen, TD and Livak, KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3, 1101–8.CrossRefGoogle Scholar
Sun, QY and Schatten, H (2006) Regulation of dynamic events by microfilaments during oocyte maturation and fertilization. Reproduction 131, 193–205.CrossRefGoogle ScholarPubMed
Wodarz, A, Ramrath, A, Kuchinke, U and Knust, E (1999) Bazooka provides an apical cue for Inscuteable localization in Drosophila neuroblasts. Nature 402, 544–7.CrossRefGoogle ScholarPubMed
Ye, Y, Kawamura, K, Sasaki, M, Kawamura, N, Groenen, P, Gelpke, MD, Rauch, R, Hseuh, AJ and Tanaka, T (2009) Kit ligand promotes first polar body extrusion of mouse preovulatory oocytes. Reprod Biol Endocrinol 7, 26.CrossRefGoogle ScholarPubMed
Yi, K, Rubinstein, B and Li, R (2013) Symmetry breaking and polarity establishment during mouse oocyte maturation. Philos Trans R Soc Lond B Biol Sci 368, 20130002.CrossRefGoogle ScholarPubMed
Zhang, Y, Wang, F, Niu, YJ, Liu, HL, Rui, R, Cui, XS, Kim, NH and Sun, SC (2015) Formin mDia1, a downstream molecule of FMNL1, regulates Profilin1 for actin assembly and spindle organization during mouse oocyte meiosis. Biochim Biophys Acta 1853, 317–27.CrossRefGoogle ScholarPubMed
Zigman, M, Cayouette, M, Charalambous, C, Schleiffer, A, Hoeller, O, Dunican, D, McCudden, CR, Finberg, N, Barres, BA, Siderovski, DP and Knoblich, JA (2005) Mammalian inscuteable regulates spindle orientation and cell fate in the developing retina. Neuron 48, 539–45.CrossRefGoogle ScholarPubMed