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Microfilament assembly and cortical granule distribution during maturation, parthenogenetic activation and fertilisation in the porcine oocyte

Published online by Cambridge University Press:  26 September 2008

Nam-Hyung Kim ,
Billy N. Day ,
Hoon Taek Lee  and
Kil-Saeng Chung
Nam-Hyung Kim
Affiliation:
Animal Resources Research Center, Kon-Kuk University, Seoul, Korea, and Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri, USA
Billy N. Day
Affiliation:
Animal Resources Research Center, Kon-Kuk University, Seoul, Korea, and Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri, USA
Hoon Taek Lee
Affiliation:
Animal Resources Research Center, Kon-Kuk University, Seoul, Korea, and Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri, USA
Kil-Saeng Chung*
Affiliation:
Animal Resources Research Center, Kon-Kuk University, Seoul, Korea, and Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri, USA
*
Dr Kil-Saeng Chung, Kon-Kuk University, Department of Animal Science, Kwangjin-gu, Mojin-dong, Seoul 143-701, South Korea. Telephone: 82-2-450-3672. Fax: 82-2-455-5305.
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Summary

In this study we imaged integral changes in microfilament assembly and cortical granule distribution, and examined effects of microfilament inhibitor on the cortical granule distribution during oocyte maturation, parthenogenetic activation and in vitro fertilisation in the pig. The microfilament assembly and cortical granule distribution were imaged with fluorescent-labelled lectin and rhodamine-labelled phalloidin under laser scanning confocal microscopy. At the germinal vesicle stage, cortical granule organelles were located around the cell cortex and were present as a relatively wide area on the oolemma. Microfilaments were also observed in a wide uniform area around the cell cortex. Following germinal vesicle breakdown, microfilaments concentrated in the condensed chromatin and cortical granules were observed in the cortex. Treatment with cytochalasin B inhibited microfilament polymerisation and prevented movement of cortical granules to the cortex. Cortical granule exudation following sperm penetration was evenly distributed in the entire perivitelline space. These results suggest that the microfilament assembly is involved in the distribution, movement and exocytosis of cortical granules during maturation and fertilisation.

Keywords

Cortical granuleFertilisationMaturationMicrofilamentsOocytePig
Type
Article
Information
Zygote , Volume 4 , Issue 2 , May 1996 , pp. 145 - 149
Copyright
Copyright © Cambridge University Press 1996

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