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lncRNA SNHG14 involved in trophoblast cell proliferation, migration, invasion and epithelial–mesenchymal transition by targeting miR-330-5p in preeclampsia

Published online by Cambridge University Press:  09 November 2020

Yulei Zhang  and
Muling Zhang Open the ORCID record for Muling Zhang [Opens in a new window]
Yulei Zhang
Affiliation:
Department of Obstetrics, Huai’an First People’s Hospital/The Affiliated Huai’an No. 1 People’s Hospital of Nanjing Medical University, Huai’an, 223300, Jiangsu, China
Muling Zhang*
Affiliation:
Department of Obstetrics, Huai’an First People’s Hospital/The Affiliated Huai’an No. 1 People’s Hospital of Nanjing Medical University, Huai’an, 223300, Jiangsu, China
*
Author for correspondence: Muling Zhang. Department of Obstetrics, Huai’an First People’s Hospital/The Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical University, No. 1 Huanghe West Road, Huaiyin District, Huai’an223300, China. E-mail: [email protected]
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Summary

Preeclampsia (PE), a pregnancy-specific disease, has become one of the leading causes of maternal and neonatal morbidity and mortality. Pathogenesis of PE has still not been fully addressed and there is a great need to develop early diagnosis markers and effective therapy. This study aimed to determine if lncRNA SNHG14 has a protective effect on placental trophoblast and prevents PE. SNHG14 levels in the peripheral blood from patients with PE or from women with healthy pregnancies were detected using RT-qPCR. The relationship between SNHG14 and miR-330-5p was determined using a dual-luciferase reporter assay. In addition, cell proliferation and cell cycle were evaluated by performing CCK8 assays and flow-cytometric analysis, respectively. Wound-healing and transwell assays were performed to assess cell migration and invasion ability. lncRNA SNHG14 was downregulated in PE patients; it was involved in trophoblast proliferation and regulated cell proliferation during G1/S transition. In addition, lncRNA SNHG14 promoted migration, invasion and epithelial–mesenchymal transition (EMT) in HTR-8/SVneo cells. Luciferase reporter assay indicated that lncRNA SNHG14 served as a molecular sponge for miR-330-5p and negatively regulated miR-330-5p expression in PE. Furthermore, the effects of silenced SNHG14 on trophoblast proliferation, migration, invasion and EMT were reversed by addition of miR-330-5p inhibitor, suggesting that in PE lncRNA SNHG14 functions by competitively binding to miR-330-5p. Taken together, the current study demonstrated that in PE lncRNA SNHG14 is a vital regulator by binding to miR-330-5p. SNHG14 might serve as a therapeutic application in PE progression.

Keywords

lncRNA SNHG14miR-330-5pPreeclampsiaTrophoblast cell
Type
Research Article
Information
Zygote , Volume 29 , Issue 2 , April 2021 , pp. 108 - 117
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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