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Laser blastocyst biopsy for preimplantation diagnosis in the human

Published online by Cambridge University Press:  26 September 2008

A. Veiga ,
M. Sandalinas ,
M. Benkhalifa ,
M. Boada ,
M. Carrera ,
J. Santaló ,
P.N. Barri  and
Y. Ménézo
A. Veiga*
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
M. Sandalinas
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
M. Benkhalifa
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
M. Boada
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
M. Carrera
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
J. Santaló
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
P.N. Barri
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
Y. Ménézo
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
*
A. Veiga, Reproductive Medicine Service, Department of Obstetrics and Gynaecology, Institut Universitari Dexeus, Barcelona, Spain.
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Summary

A new methodology for blastocyst biopsy that uses a 1.48μm in diode laser is described. Trophectoderm cells are biopsied after laster zona drilling and culture, fixed and processed for fluorescent in situ hybridisation (FISH) analysis. Preliminary results on the efficiency of the procedure and blastocyst recovery rate are promising. Blastocyst laser biopsy is a useful tool in preimplantation genetic diagnosis (PGD) as it allows a more reliable diagnosis and widens the diagnostic possibilities on account of the higher number of cells obtained in the biopsy.

Keywords

BlastocystNon-contact laserPreimplantation genetic diagnosisTrophectoderm biopsy
Type
Article
Information
Zygote , Volume 5 , Issue 4 , November 1997 , pp. 351 - 354
Copyright
Copyright © Cambridge University Press 1997

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