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Investigation of histone H4 hyperacetylation dynamics in the 5S rRNA genes family by chromatin immunoprecipitation assay

Published online by Cambridge University Press:  15 October 2014

Liliana Burlibașa  and
Ilinca Suciu
Liliana Burlibașa*
Affiliation:
University of Bucharest, Genetics Department, Bucharest, Romania. University of Bucharest, Genetics Department, Bucharest, Romania.
Ilinca Suciu
Affiliation:
University of Bucharest, Genetics Department, Bucharest, Romania.
*
All correspondence to: Liliana Burlibașa. University of Bucharest, Genetics Department, Bucharest, Romania. e-mail: [email protected]
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Summary

Oogenesis is a critical event in the formation of female gamete, whose role in development is to transfer genomic information to the next generation. During this process, the gene expression pattern changes dramatically concomitant with genome remodelling, while genomic information is stably maintained. The aim of the present study was to investigate the presence of H4 acetylation of the oocyte and somatic 5S rRNA genes in Triturus cristatus, using chromatin immunoprecipitation assay (ChIP). Our findings suggest that some epigenetic mechanisms such as histone acetylation could be involved in the transcriptional regulation of 5S rRNA gene families.

Keywords

5S rDNAoocytesChIP assayH4 hyperacetylationTriturus cristatus
Type
Short Communication
Information
Zygote , Volume 23 , Issue 6 , December 2015 , pp. 951 - 954
Copyright
Copyright © Cambridge University Press 2014 

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References

Agalioti, T., Chen, G. & Thanos, D. (2002). Deciphering the transcriptional histone acetylation code for a human gene. Cell 111, 381–92.Google Scholar
Burlibașa, L. & Zarnescu, O. (2013). In vivo effects of trichostatin A—a histone deacetylase inhibitor–on chromatin remodeling during Triturus cristatus spermatogenesis. Anim. Rep. Sci. 142, 89–99.Google Scholar
Burlibașa, L., Zarnescu, O., Cucu, N. & Gavrilă, L. (2008). Chromatin dynamics in Triturus cristatus oogenesis – an epigenetic approach, Zygote 16, 315–26.Google Scholar
Chipev, C.C. & Wolffe, A.P. (1992). Chromosomal organization of Xenopus laevis oocyte and somatic 5S rRNA genes in vivo . Mol. Cell. Biol. 12, 45–55.Google Scholar
Howe, L.A., Ranalli, T.A., Allis, D.C. & Ausio, J. (1998). Transcriptionally active Xenopus laevis somatic 5S ribosomal RNA genes are packaged with hyperacetylated histone H4, whereas transcriptionally silent oocyte genes are not. J. Biol. Chem. 273, 20693–6.Google Scholar
Lawrence, R.J., Early, K., Pontes, O., Silva, M., Chen, Z.J., Neves, N., Viegas, W. & Pikaard, C.S. (2004). A concerted DNA methylation/histone methylation switch regulates rRNA gene dosage control and nucleolar dominance. Mol. Cells 13, 599–609.Google Scholar
McStay, B. (2006). Nucleolar dominance, a model for rRNA gene silencing. Genes Dev. 20, 1207–14.Google Scholar
Reeder, R.H. (1985). Mechanisms of nucleolar dominance in animals and plants. J. Cell Biol. 101, 2013–6.Google Scholar
Reynolds, W.F., Smith, R.D., Bloomer, L.S. & Gottesfeld, J.M. (1982). Organization of Xenopus 5-s-genes in chromatin. Cell Nucleus 11, 63–87.Google Scholar
Santoro, R. & Grummt, I. (2005). Epigenetic mechanism of rRNA gene silencing, temporal order of NoRC-mediated histone modification, chromatin remodelling and DNA methylation. Mol. Cell. Biol. 25, 2539–46.Google Scholar
Santoro, R., Li, J. & Grummt, I. (2002). The nucleolar remodelling complex NoRC mediates heterochromatin formation and silencing of ribosomal gene transcription. Nat. Genet. 32, 393–6.Google Scholar
Shestakova, E., Bandu, M.T., Doly, J. & Bonnefoy, E. (2001). Inhibition of histone induces constitutive depression of the b interferon promoter and confers antiviral activity. J. Virol. 75, 3444–52.Google Scholar
Workman, J.L. (2006). Nucleosome displacement in transcription, Genes Dev. 20, 2009–17.Google Scholar