Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-29T19:09:10.699Z Has data issue: false hasContentIssue false
  • English
  • Français

Insulin–transferrin–selenium (ITS) improves maturation of porcine oocytes in vitro

Published online by Cambridge University Press:  08 February 2011

Junhe Hu ,
Xiaoling Ma ,
Jian Chang Bao ,
Wei Li ,
De Cheng ,
Zhimin Gao ,
Anmin Lei ,
Chunrong Yang  and
Huayan Wang
Junhe Hu
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China. Department of Life Science, Yulin College, Yulin, Shaanxi 719000, China.
Xiaoling Ma
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Jian Chang Bao
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Wei Li
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
De Cheng
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Zhimin Gao
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Anmin Lei
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Chunrong Yang
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Huayan Wang*
Affiliation:
Shaanxi Center for Stem Cell Engineering and Technology, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China. College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
*
All correspondence to: Huayan Wang, Shaanxi Center for Stem Cell Engineering and Technology, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China. Tel: +86 029 87080069. Fax: +86 029 87080068. e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

The objective of this study was to determine if insulin–transferrin–selenium (ITS) promoted a nuclear and cytoplasmic maturation of porcine oocytes that better supports subsequent embryonic development. The rate of oocyte in vitro maturation (IVM) in an experimental group treated with hormones for 42 h was significantly increased compared with that in a control group without hormone treatment (47.8% vs. 11.7%, respectively, p < 0.05). Following reduction of the hormone treatment period from 42 h to 21 h, which included both the first 21 h period of hormones treatment (45.4%) and the second 21 h period of hormone treatment (44.8%), the rate of oocyte IVM was still higher than that of the control group (p < 0.05). To improve porcine oocyte nuclear maturation, 1% ITS was added to medium supplemented with hormones. The rate of nuclear maturation in the ITS-treated group was significantly higher than in the ITS-untreated group (78.6% vs. 54.4%, respectively, p < 0.05). ITS treatment also significantly reduced the per cent of oocytes with type I and type III cortical granule (CG) distribution, respectively, and significantly increased the per cent of oocytes with type II CG distribution (85.3%). These observations indicated that the synchronization rates of nuclear and ooplasmic maturation reached 67.04% (78.56 × 85.33%). In conclusion, the combination of modified Tissue Culture Medium-199 (mM199) + 10 ng/ml epidermal growth factor (EGF) + 10 IU/ml pregnant mare serum gonadotrophin (PMSG) + 10 IU/ml human chorion gonadotrophin (hCG) + 2.5 IU/ml follicle stimulating hormone (FSH) + 1% ITS is suitable for culturing porcine oocytes in vitro, and effectively enhances porcine oocyte nuclear and cytoplasmic maturation.

Keywords

Cortical granulesIn vitro maturationInsulin–transferrin–seleniumPorcinePorcine oocyte
Type
Research Article
Information
Zygote , Volume 19 , Issue 3 , August 2011 , pp. 191 - 197
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cao, X., Zhou, P., Luo, H., Zhao, Y. & Shi, G. (2009). The effect of VEGF on the temporal-spatial change of alpha-tubulin and cortical granules of ovine oocytes matured in vitro. Anim. Reprod. Sci. 113, 236–50.CrossRefGoogle ScholarPubMed
Cerri, R.L., Rutigliano, H.M., Lima, F.S., Araujo, D.B. & Santos, J.E. (2009). Effect of source of supplemental selenium on uterine health and embryo quality in high-producing dairy cows. Theriogenology 71, 1127–37.CrossRefGoogle ScholarPubMed
De La Fuente, R., O'Brien, M.J. & Eppig, J.J. (1999). Epidermal growth factor enhances preimplantation developmental competence of maturing mouse oocytes. Hum. Reprod. 14, 3060–8.CrossRefGoogle ScholarPubMed
Favetta, L.A., St John, E.J., King, W.A. & Betts, D.H. (2007). High levels of p66shc and intracellular ROS in permanently arrested early embryos. Free Radic. Biol. Med. 42, 1201–10.CrossRefGoogle ScholarPubMed
Ferreira, E.M., Vireque, A.A., Adona, P.R., Meirelles, F.V., Ferriani, R.A. & Navarro, P.A. (2009). Cytoplasmic maturation of bovine oocytes: structural and biochemical modifications and acquisition of developmental competence. Theriogenology 71, 836–48.CrossRefGoogle ScholarPubMed
Funahashi, H. & Day, B.N. (1993). Effects of the duration of exposure to hormone supplements on cytoplasmic maturation of pig oocytes in vitro. J. Reprod. Fertil. 98, 179–85.CrossRefGoogle ScholarPubMed
Funahashi, H., Cantley, T. & Day, B.N. (1994). Different hormonal requirements of pig oocyte–cumulus complexes during maturation in vitro. J. Reprod. Fertil. 101, 159–65.CrossRefGoogle ScholarPubMed
Herrick, J.R., Behboodi, E., Memili, E., Blash, S., Echelard, Y. & Krisher, R.L. (2004). Effect of macromolecule supplementation during in vitro maturation of goat oocytes on developmental potential. Mol. Reprod. Dev. 69, 338–46.CrossRefGoogle ScholarPubMed
Hosoe, M. & Shioya, Y. (1997). Distribution of cortical granules in bovine oocytes classified by cumulus complex. Zygote 5, 371–6.CrossRefGoogle ScholarPubMed
Jeong, Y.W., Hossein, M.S., Bhandari, D.P., Kim, Y.W., Kim, J.H., Park, S.W., Lee, E., Park, S.M., Jeong, Y.I., Lee, J.Y., Kim, S. & Hwang, W.S. (2008). Effects of insulin–transferrin–selenium in defined and porcine follicular fluid supplemented IVM media on porcine IVF and SCNT embryo production. Anim. Reprod. Sci. 106, 13–24.CrossRefGoogle ScholarPubMed
Kawashima, I., Okazaki, T., Noma, N., Nishibori, M., Yamashita, Y. & Shimada, M. (2008). Sequential exposure of porcine cumulus cells to FSH and/or LH is critical for appropriate expression of steroidogenic and ovulation-related genes that impact oocyte maturation in vivo and in vitro. Reproduction 136, 9–21.CrossRefGoogle ScholarPubMed
Kim, S., Lee, G.S., Lee, S.H., Kim, H.S., Jeong, Y.W., Kim, J.H., Kang, S.K., Lee, B.C. & Hwang, W.S. (2005). Embryotropic effect of insulin-like growth factor (IGF)-I and its receptor on development of porcine preimplantation embryos produced by in vitro fertilization and somatic cell nuclear transfer. Mol. Reprod. Dev. 72, 88–97.CrossRefGoogle ScholarPubMed
Lee, M.S., Kang, S.K., Lee, B.C. & Hwang, W.S. (2005). The beneficial effects of insulin and metformin on in vitro developmental potential of porcine oocytes and embryos. Biol. Reprod. 73, 1264–8.CrossRefGoogle ScholarPubMed
Liu, X.Y., Mal, S.F., Miao, D.Q., Liu, D.J., Bao, S. & Tan, J.H. (2005). Cortical granules behave differently in mouse oocytes matured under different conditions. Hum. Reprod. 20, 3402–13.CrossRefGoogle ScholarPubMed
Lonergan, P., Rizos, D., Gutierrez-Adan, A., Fair, T. & Boland, M.P. (2003). Effect of culture environment on embryo quality and gene expression – experience from animal studies. Reprod. Biomed. Online 7, 657–63.CrossRefGoogle ScholarPubMed
Rodrigues, B.A. & Rodrigues, J.L. (2003). Meiotic response of in vitro matured canine oocytes under different proteins and heterologous hormone supplementation. Reprod. Domest. Anim. 38, 58–62.CrossRefGoogle ScholarPubMed
Shimada, M., Nishibori, M., Isobe, N., Kawano, N. & Terada, T. (2003). Luteinizing hormone receptor formation in cumulus cells surrounding porcine oocytes and its role during meiotic maturation of porcine oocytes. Biol. Reprod. 68, 1142–9.CrossRefGoogle ScholarPubMed
Tatemoto, H., Muto, N., Sunagawa, I., Shinjo, A. & Nakada, T. (2004). Protection of porcine oocytes against cell damage caused by oxidative stress during in vitro maturation: role of superoxide dismutase activity in porcine follicular fluid. Biol. Reprod. 71, 1150–7.CrossRefGoogle ScholarPubMed
Viana, K.S., Caldas-Bussiere, M.C., Matta, S.G., Faes, M.R., de Carvalho, C.S. & Quirino, C.R. (2007). Effect of sodium nitroprusside, a nitric oxide donor, on the in vitro maturation of bovine oocytes. Anim. Reprod. Sci. 102, 217–27.CrossRefGoogle ScholarPubMed
Wang, W., Hosoe, M., Li, R. & Shioya, Y. (1997a). Development of the competence of bovine oocytes to release cortical granules and block polyspermy after meiotic maturation. Dev. Growth Differ. 39, 607–15.CrossRefGoogle ScholarPubMed
Wang, W.H., Sun, Q.Y., Hosoe, M., Shioya, Y. & Day, B.N. (1997b). Quantified analysis of cortical granule distribution and exocytosis of porcine oocytes during meiotic maturation and activation. Biol. Reprod. 56, 1376–82.CrossRefGoogle ScholarPubMed