Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-19T03:54:30.203Z Has data issue: false hasContentIssue false
  • English
  • Français

The influence of clinical and laboratory factors on the formation of monopronucleated zygotes after intracytoplasmic sperm injection (ICSI)

Published online by Cambridge University Press:  25 February 2019

Gemma Fabozzi Open the ORCID record for Gemma Fabozzi [Opens in a new window] ,
Emilia Rega ,
Maria Flavia Starita ,
Maria Giulia Amendola ,
Antonio Colicchia ,
Pierluigi Giannini  and
Claudio Piscitelli
Gemma Fabozzi*
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
Emilia Rega
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
Maria Flavia Starita
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
Maria Giulia Amendola
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
Antonio Colicchia
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
Pierluigi Giannini
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
Claudio Piscitelli
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
*
Author for correspondence: Gemma Fabozzi, FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy. Tel: +39 06 94443140. Fax: +39 06 86275838. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

The aim of the present study was to determine whether clinical or laboratory factors can influence the development of single pronucleated zygotes (1PN) and two polar bodies (PB) after ICSI. In total, 341 ICSI cycles performed at FertiClinic-Villa Margherita from January 2012 to December 2014 were enrolled in the study. Group A included 240 cycles with no 1PN−2PB while group B included 101 cycles with one or more 1PN−2PB. Age, stimulation protocol, infertility factor, amount of gonadotropin administered, duration of therapy, peak estradiol levels, number of follicles at maturation triggering, oocytes retrieved and mature oocytes, time between retrieval and injection and sperm characteristics were compared between groups. In opposition to previous results showing no relationship between 1PN occurrence and clinical or laboratory variables, we observed that 1PN−2PB zygote formation seems to be associated with a lower female age, higher level of E2 and higher number of follicles on day of oocyte maturation triggering, higher number of astenozoospermic male patients, more oocytes retrieved at pick-up, more mature oocytes (MII) and longer time to injection.

Keywords

GonadotropinsICSIMale infertilityMonopronucleated zygotesStimulation protocol.
Type
Research Article
Information
Zygote , Volume 27 , Issue 2 , April 2019 , pp. 64 - 68
Copyright
© Cambridge University Press 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alpha Scientists in Reproductive Medicine ESHRE Special Interest Group of Embryology (2011) The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod 26, 1270–1283 and Reprod Biomed Online 22, 1632–46 (simultaneous publication).Google Scholar
Azevedo, AR, Pinho, MJ, Silva, J, Sá, R, Thorsteinsdóttir, S, Barros, A Sousa, MAzevedo (2014) Molecular cytogenetics of human single pronucleated zygotes. Reprod Sci 21, 1472–1482.Google Scholar
Baart, EB, Martini, E, Eijkemans, MJ, Van Opstal, D, Beckers, NG, Verhoeff, A, Macklon, NS Fauser, BC (2007) Milder ovarian stimulation for in-vitro fertilization reduces aneuploidy in the human preimplantation embryo: a randomized controlled trial. Hum Reprod 22, 980–988.Google Scholar
Balakier, H Casper, RF (1991) A morphologic study of unfertilized oocytes and abnormal embryos in human in vitro fertilization. J In Vitro Fert Embryo Transf 8, 73–79.Google Scholar
Balakier, H, Squire, J Casper, RF (1993) Characterization of abnormal one pronuclear human oocytes by morphology, cytogenetics and in-situ hybridization. Hum Reprod 8, 402–408.Google Scholar
Balhorn, R, Reed, S Tanphaichitr, N (1988) Aberrant protamine 1/protamine 2 ratios in sperm of infertile human males. Experientia 44, 52–55.Google Scholar
Belokopytova, IA, Kostyleva, EI, Tomilin, AN Vorob’ev, VI (1993) Human male infertility may be due to a decrease of the protamine P2 content in sperm chromatin. Mol Reprod Dev 34, 53–57.Google Scholar
Bradley, CK, Traversa, MV, Hobson, N, Gee, AJ McArthur, SJ (2017) Clinical use of monopronucleated zygotes following blastocyst culture and preimplantation genetic screening, including verification of biparental chromosome inheritance. Reprod BioMed Online 34, 567–574.Google Scholar
Capalbo, A, Treff, N, Cimadomo, D, Tao, X, Ferrero, S, Vaiarelli, A, Colamaria, S, Maggiulli, R, Orlando, G, Scarica, C, Scott, R, Ubaldi, FM Rienzi, L (2017) Abnormally fertilized oocytes can result in healthy live births: improved genetic technologies for preimplantation genetic testing can be used to rescue viable embryos in in vitro fertilization cycles. Fertil Steril 108, 1007–1015.Google Scholar
De Placido, G, Wilding, M, Strina, I, Alviggi, E, Alviggi, C, Mollo, A, Varicchio, MT, Tolino, A, Schiattarella, C Dale, B (2002) High outcome predictability after IVF using a combined score for zygote and embryo morphology and growth rate. Hum Reprod 17, 2402–2409.Google Scholar
de Yebra, L, Ballescà, JL, Vanrell, JA, Bassas, L Oliva, R (1993) Complete selective absence of protamine P2 in humans. J Biol Chem 268, 10553–10557.Google Scholar
Delvigne, A (2009) Symposium: Update on prediction and management of OHSS. Epidemiology of OHSS. Reprod Biomed Online 19, 8–13.Google Scholar
Ectors, FJ, Vanderzwalmen, P, Van Hoeck, J, Nijs, M, Verhaegen, G, Delvigne, A, Schoysman, R Leroy, F (1997) Relationship of human follicular diameter with oocyte fertilization and development after in-vitro fertilization or intracytoplasmic sperm injection. Hum Reprod 12, 2002–2005.Google Scholar
Griveau, JF, Charbonneau, M, Blanchard, Y, Lescoat, D Le Lannou, D (1992) Decondensation of human sperm nuclei and HP1 protamine degradation from normospermia and asthenospermia in Xenopus egg extracts. Arch Androl 29, 127–136.Google Scholar
Levron, J, Munné, S, Willadsen, S, Rosenwaks, Z Cohen, J (1995) Male and female genomes associated in a single pronucleus in human zygotes. Biol Reprod 52, 653–657.Google Scholar
Li, M, Xue, X, Zhang, S, Li, W, Zhao, X, Ren, W Shi, J (2015) Effects of triploidy incidence on clinical outcomes for IVF-ET cycles in different ovarian stimulation protocols. Gynecol Endocrinol 31, 769–773.Google Scholar
Lim, AS, Goh, VH, Su, CL Yu, SL (2000) Microscopic assessment of pronuclear embryos is not definitive. Hum Genet 107, 62–68.Google Scholar
Mateo, S, Parriego, M, Boada, M, Vidal, F, Coroleu, B Veiga, A (2013) In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection. Fertil Steril 99, 897–902.Google Scholar
Mateo, S, Vidal, F, Parriego, M, Rodríguez, I, Montalvo, V, Veiga, A Boada, M (2017) Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS. J Assist Reprod Genet 34, 905–911.Google Scholar
Munné, S Cohen, J (1998) Chromosome abnormalities in human embryos. Hum Reprod Update 4, 842–855.Google Scholar
Munné, S, Tang, Y, Grifo, J Cohen, J (1993) Origin of single pronucleated human zygotes. J Assist Reprod Genet 10, 276–279.Google Scholar
Miao, YL, Kikuchi, K, Sun, QY Schatten, H (2009) Oocyte aging: cellular and molecular changes, developmental potential and reversal possibility. Hum Reprod Update 15, 573–585.Google Scholar
Nogueira, D, Friedler, S, Schachter, M, Raziel, A, Ron-El, R Smitz, J (2006) Oocyte maturity and preimplantation development in relation to follicle diameter in gonadotropin-releasing hormone agonist or antagonist treatments. Fertil Steril 85, 578–583.Google Scholar
Otsu, E, Sato, A, Nagaki, M, Araki, Y Utsunomiya, T (2004) Developmental potential and chromosomal constitution of embryos derived from larger single pronuclei of human zygotes used in vitro fertilization. Fertil Steril 81, 723–724.Google Scholar
Palermo, G, Joris, H, Derde, MP, Camus, M, Devroey, P Van Steirteghem, A (1993) Sperm characteristics and outcome of human assisted fertilization by subzonal insemination and intracytoplasmic sperm injection. Fertil Steril 59, 826–835.Google Scholar
Plachot, M (1991) Chromosome analysis of oocytes and embryos. In Verlinsky Y and Kuliev A (eds). Preimplantation Genetics Springer, Boston, MA.Google Scholar
Plachot, M Crozet, N (1992) Fertilization abnormalities in human in-vitro fertilization. Hum Reprod 7(Suppl 1), 89–94.Google Scholar
Rienzi L, Ubaldi F, Anniballo R, Cerulo G and Greco E (1998) Preincubation of human oocytes may improve fertilization and embryo quality after intracytoplasmic sperm injection. Hum Reprod 13, 1014–9.Google Scholar
Rizk, B (2009) Symposium: Update on prediction and management of OHSS. Genetics of ovarian hyperstimulation syndrome. Reprod Biomed Online 19, 14–27.Google Scholar
Rosen, MP, Shen, S, Dobson, AT, Fujimoto, VY, McCulloch, CE Cedars, MI (2006) Triploidy formation after intracytoplasmic sperm injection may be a surrogate marker for implantation. Fertil Steril 85, 384–390.Google Scholar
Sachs, AR, Politch, JA, Jackson, KV, Racowsky, C, Hornstein, MD Ginsburg, ES (2000) Factors associated with the formation of triploid zygotes after intracytoplasmic sperm injection. Fertil Steril 73, 1109–1114.Google Scholar
Scott, RT Jr Rosenwaks, Z Ovulation induction for assisted reproduction. (1989) J Reprod Med 34(1 Suppl):108–114.Google Scholar
Staessen, C Van Steirteghem, C (1997) The chromosomal constitution of embryos developing from abnormally fertilized oocytes after intracytoplasmic sperm injection and conventional in-vitro fertilization. Hum Reprod 12, 321–327.Google Scholar
Staessen, C, Janssenswillen, C, Devroey, P Van Steirteghem, AC (1993) Cytogenetic and morphological observations of single pronucleated human oocytes after in-vitro fertilization. Hum Reprod 8, 221–223.Google Scholar
Sultan, KM, Munne, S, Palermo, GD, Alikani, M Cohen, J (1995) Chromosomal status of uni-pronuclear human zygotes following in-vitro fertilization and intracytoplasmic sperm injection. Hum Reprod 10, 132–136.Google Scholar
Van der Heijden, GW, van den Berg, IM, Baart, EB, Derijck, AA, Martini, E de Boer, P (2009) Parental origin of chromatin in human monopronuclear zygotes revealed by asymmetric histone methylation patterns, differs between IVF and ICSI. Mol Reprod Dev 76, 101–108.Google Scholar