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Incidence of apoptosis after retinoids and insulin-like growth factor-I (IGF-I) supplementation during goat in vitro embryo production

Published online by Cambridge University Press:  17 June 2016

Juliana C.Z. Conceição ,
Marcelo T. Moura ,
José C. Ferreira-Silva ,
Ludymila F. Cantanhêde ,
Ricardo M. Chaves ,
Paulo F. Lima  and
Marcos A.L. Oliveira
Juliana C.Z. Conceição
Affiliation:
Laboratório de Biotécnicas Reprodutivas do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco (UFRPE). Av. Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171–900 Recife-PE/Brasil.
Marcelo T. Moura
Affiliation:
Laboratório de Biotécnicas Reprodutivas do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco (UFRPE). Av. Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171–900 Recife-PE/Brasil.
José C. Ferreira-Silva
Affiliation:
Laboratório de Biotécnicas Reprodutivas do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco (UFRPE). Av. Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171–900 Recife-PE/Brasil.
Ludymila F. Cantanhêde
Affiliation:
Laboratório de Biotécnicas Reprodutivas do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco (UFRPE). Av. Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171–900 Recife-PE/Brasil.
Ricardo M. Chaves
Affiliation:
Laboratório de Reprodução Animal da Universidade Estadual do Maranhão, São Luis-MA/Brasil.
Paulo F. Lima
Affiliation:
Laboratório de Biotécnicas Reprodutivas do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco (UFRPE). Av. Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171–900 Recife-PE/Brasil.
Marcos A.L. Oliveira*
Affiliation:
Laboratório de Biotécnicas Reprodutivas do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco (UFRPE). Av. Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171–900 Recife-PE/Brasil.
*
All correspondence to: Marcos A.L. Oliveira. Laboratório de Biotécnicas Reprodutivas do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco (UFRPE). Av. Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171–900 Recife-PE/Brasil. Tel:/Fax: +55 81 3320-6415. E-mail: [email protected]
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Summary

The addition of growth factors and vitamins enhances goat embryonic development in vitro. However, few attempts have been reported trying to identify supplementation regimens for oocyte maturation or embryo culture with additive properties. The present report was aimed to evaluate if retinoids [0.3 μM retinyl acetate (RAc) and 0.5 μM 9-cis-retinoic acid (RA)] supplementation during goat oocyte maturation and retinoids and/or 50 ng mL–1 IGF-I during embryo culture synergically enhanced embryonic development while diminishing the incidence of apoptosis. All combinations of RAc and RA treatment produced blastocysts with similar efficiencies, while IGF-I enhanced embryos yields irrespectively of retinoid addition. Moreover, retinoids and IGF-I supplementation showed similar caspase activity or DNA fragmentation indexes in blastocysts. In conclusion, supplementation with retinoids and IGF-I during goat embryo culture enhances blastocysts development without synergic reduction of apoptosis.

Keywords

GoatIVFPreimplantationProgrammed cell deathVitamin A
Type
Research Article
Information
Zygote , Volume 24 , Issue 6 , December 2016 , pp. 808 - 813
Copyright
Copyright © Cambridge University Press 2016 

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