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In vitro maturation, in vitro fertilization and embryonic development of canine oocytes

Published online by Cambridge University Press:  01 November 2007

S.R. Lee ,
B.S. Kim ,
J-W. Kim ,
M.O. Kim ,
S.H. Kim ,
D.H. Yoo ,
M-J. Shin ,
Y.S. Park ,
S. Lee  and
Y.B. Park
...Show all authors
S.R. Lee
Affiliation:
School of Life Science and Biotechnology, Kyungpook National University, Daegu 702–701, Republic of Korea.
B.S. Kim
Affiliation:
School of Life Science and Biotechnology, Kyungpook National University, Daegu 702–701, Republic of Korea.
J-W. Kim
Affiliation:
School of Life Science and Biotechnology, Kyungpook National University, Daegu 702–701, Republic of Korea.
M.O. Kim
Affiliation:
School of Life Science and Biotechnology, Kyungpook National University, Daegu 702–701, Republic of Korea.
S.H. Kim
Affiliation:
School of Life Science and Biotechnology, Kyungpook National University, Daegu 702–701, Republic of Korea.
D.H. Yoo
Affiliation:
School of Life Science and Biotechnology, Kyungpook National University, Daegu 702–701, Republic of Korea.
M-J. Shin
Affiliation:
School of Life Science and Biotechnology, Kyungpook National University, Daegu 702–701, Republic of Korea.
Y.S. Park
Affiliation:
Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702–701, Republic of Korea.
S. Lee
Affiliation:
School of Life Science and Biotechnology, Kyungpook National University, Daegu 702–701, Republic of Korea.
Y.B. Park
Affiliation:
School of Life Science and Biotechnology, Kyungpook National University, Daegu 702–701, Republic of Korea.
J.H. Ha
Affiliation:
School of Life Science and Biotechnology, Kyungpook National University, Daegu 702–701, Republic of Korea.
Z.Y. Ryoo*
Affiliation:
School of Life Science and Biotechnology, Kyungpook National University, Daegu 702–701, Republic of Korea.
*
Correspondence and requests for materials should be addressed to: Zae Young Ryoo. School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, Daegu 702–701, Korea. Tel: +82 53 950 7361. Fax: +82 53 943 6925. e-mail: [email protected]
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Summary

In this study we have investigated the efficiency of in vitro maturation (IVM) as a basic way to study the development of canine oocytes after in vitro fertilization (IVF). We decided, therefore, to perform two-part experiments. Firstly, experiment I compared the effects of TCM199 without fetal bovine serum (FBS) with TCM199 supplemented with 5% FBS on the in vitro nuclear maturation rate of canine oocytes. For the efficiency of meiotic development to the metaphase II (MII) stage, we found that 4.7% (4/64) of all oocytes grown in TCM199 without FBS developed to the MII stage compared with only 1.7% (1/59) of those grown in TCM199 with 5% FBS for 48 h. Therefore, FBS did not increase in vitro nuclear maturation. In experiment II, the cleavage rate of canine oocytes used for IVF was investigated following heparin treatment. Canine oocytes were fertilized in four groups: Fert–TALP medium without heparin (Fert I) or Fert–TALP medium supplemented with 10, 20 or 30 µg/ml heparin (Fert II, Fert III, Fert IV, respectively). Oocytes that were grown for 24 h in Fert I following fertilization showed the highest rate of all of the groups, 6.5% (5/77) and developed to the early morula stage. Markedly, the oocytes cultured in Fert I for 24 h following insemination had a higher rate of embryonic development than other groups. We can assert that, unlike findings in other mammals, heparin treatment in canine IVF does not increase the efficiency of the fertilization rate and is therefore not an important factor.

Keywords

CanineEmbryoIn vitro fertilizationIn vitro maturationOocyte
Type
Research Article
Information
Zygote , Volume 15 , Issue 4 , November 2007 , pp. 347 - 353
Copyright
Copyright © Cambridge University Press 2007

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