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Improvement of the developmental competence of canine oocyte using caffeine supplementation during IVM at different maturation time

Published online by Cambridge University Press:  25 May 2018

Mohamed Fathi ,
A. Salama  and
Magdy R. Badr
Mohamed Fathi*
Affiliation:
Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
A. Salama
Affiliation:
Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Magdy R. Badr
Affiliation:
Department of AI and ET, Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt.
*
All correspondence to: Mohamed Fathi. Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. E-mail: [email protected]
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Summary

The aim of the current study was to investigate the effect of caffeine supplementation during in vitro maturation (IVM) for different maturation times on the developmental potential of canine oocytes recovered from ovariohysterectomized bitches. The recovered cumulus–oocytes complexes were in vitro matured for 72 h. Here, 10 mM caffeine was added to the maturation medium for different incubation times (caffeine from 0–72 h maturation, caffeine for the first 24 h of maturation only, caffeine addition from 24 to 48 h maturation time, caffeine addition from 48 to 72 h maturation or in caffeine-free medium, control group). The matured oocytes were in vitro fertilized using frozen–thawed spermatozoa. The presumptive zygotes were in vitro cultured in synthetic oviductal fluid medium for 5 days. The results showed that both maturation and fertilization rates were significantly higher (P ˂ 0.05) using caffeine-treated medium for the first 24 h of maturation compared with the control and other two groups of caffeine treatment (from 24 to 48 h and from 48 to 72 h), whereas use of caffeine-treated medium for a 0–72 h incubation time did not affect these rates (P > 0.05). Interestingly, the matured oocytes in caffeine-supplemented medium for the first 24 h or from 0–72 h showed a significant (P ˂ 0.05) increase in the total number of cleaved embryos compared with the control group. In conclusion, supplementation of the maturation medium with 10 mM caffeine for the first 24 h of maturation or during the whole maturation time (0–72 h) improved nuclear maturation and subsequent embryo development preimplantation following in vitro fertilization.

Keywords

CaffeineCanine oocytesIVCIVFIVM
Type
Research Article
Information
Zygote , Volume 26 , Issue 2 , April 2018 , pp. 162 - 167
Copyright
Copyright © Cambridge University Press 2018 

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