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Impact of insemination technique, semen quality and oocyte cryopreservation on pronuclear morphology of zygotes derived from sibling oocytes

Published online by Cambridge University Press:  08 June 2009

Alessia Nicoli ,
Francesco Capodanno ,
Barbara Valli ,
Roberta Di Girolamo ,
Maria Teresa Villani ,
Andrea Nucera ,
Riccardo Focarelli  and
Giovanni B. La Sala
Alessia Nicoli*
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, 42100 Reggio Emilia, Italy. Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy.
Francesco Capodanno
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy.
Barbara Valli
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy.
Roberta Di Girolamo
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy.
Maria Teresa Villani
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy.
Andrea Nucera
Affiliation:
Department of Health Sciences, Section of Medical Statistics and Epidemiology, University of Pavia, Italy.
Riccardo Focarelli
Affiliation:
Department of Evolutionary Biology, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy.
Giovanni B. La Sala
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy.
*
All correspondence to: Alessia Nicoli. Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, 42100 Reggio Emilia, Italy. Tel: +39 0522296354. Fax: +39 522295909. e-mail: [email protected]
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Summary

Pronuclear morphology seems to be an important predictive value of zygote development and integrity. In this study we want to evaluate the effect of insemination technique, male factor and oocyte cryopreservation on pronuclear morphology of zygotes derived from sibling oocytes in our Centre of Reproductive Medicine, Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy. Subjects (n = 190) were submitted to IVF cycles with non-frozen and frozen sibling oocytes. Morphological evaluations were assessed using zygote pronuclear morphology (pronuclei, nucleoli and axis) in four groups: Group 1: 144 zygotes from 85 conventional IVF cycles with non-frozen oocytes; Group 2: 164 zygotes from 85 intracytoplasmic sperm injection (ICSI) cycles with Group 1 patients' sibling frozen oocytes; Group 3: 221 zygotes from 123 ICSI cycles with non-frozen oocytes; Group 4: 197 zygotes from 123 ICSI cycles with Group 3 patients' sibling frozen oocytes. No differences between Group 1 and Group 2 were seen. Group 3 was statistically different from Group 4 in relation to the nucleolar morphology. Oocyte cryopreservation procedure modified the nucleolar morphology of zygotes only in the presence of poor semen quality.

Keywords

CryopreservationICSIIVFPronucleiZygote
Type
Research Article
Information
Zygote , Volume 18 , Issue 1 , February 2010 , pp. 61 - 68
Copyright
Copyright © Cambridge University Press 2009

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