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Glycine and methionine transport by bovine embryos

Published online by Cambridge University Press:  26 September 2008

Catherine Guyader-Joly ,
Chaqué Khatchadourian  and
Yves Ménézo
Catherine Guyader-Joly
Affiliation:
UNCEIA, Chateauvillain; INRA, Jouy-en-Josas; and INSA, Villeurbanne, France
Chaqué Khatchadourian
Affiliation:
UNCEIA, Chateauvillain; INRA, Jouy-en-Josas; and INSA, Villeurbanne, France
Yves Ménézo*
Affiliation:
UNCEIA, Chateauvillain; INRA, Jouy-en-Josas; and INSA, Villeurbanne, France
*
Y. Ménézo, INSA, Unité de Biologie du développement préimplantatoire, Laboratoire de Biologie Appliquée, Bâtiment 406, 20 avenue Albert Einstein, 69621 Villeurbanne Cedex, France. Telephone: +33/04 72 43 83 39. Fax: +33/04 72 43 85 11.
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Summary

As glycine is one of the most concentrated amino acids in the female genital tract, we investigated its uptake by bovine in vitro matured/in vitro fertilised blastocysts in the presence of increasing concentrations of radiolabelled glycine. We also determined methionine uptake by in vitro and in vivo produced embryos. In our study, the hypothesis of more than one site of enzyme activity for glycine substrate was not validated. We determined a Vmax of 23.4fmol/min per embryo and a Km value of 13.3μM. No significant difference was observed either between in vivo and in vitro derived embryos or between grade 1 and grade 2 embryos for methionine uptake. The methionine and glycine uptake of a day 7 bovine was similar to that of a day 4 mouse blastocyst. This is rather low if we consider the relative cell numbers.

Keywords

Glycine and methionine transportBovine blastocyst
Type
Article
Information
Zygote , Volume 5 , Issue 3 , August 1997 , pp. 273 - 276
Copyright
Copyright © Cambridge University Press 1997

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