Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-27T01:25:01.676Z Has data issue: false hasContentIssue false
  • English
  • Français

Full term development of normal mice after transfer of IVF embryos derived from oocytes stored at room temperature for 1 day

Published online by Cambridge University Press:  01 February 2008

Zi-Li Lei ,
Jun-Cheng Huang ,
Li-Hong Shi ,
Yi-Liang Miao ,
Chang-Long Nan ,
Ji-Wen Yang ,
Ying-Chun OuYang ,
Qing-Yuan Sun  and
Da-Yuan Chen
Zi-Li Lei
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China. Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Jun-Cheng Huang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China. Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Li-Hong Shi
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China. Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Yi-Liang Miao
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China. Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Chang-Long Nan
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China. Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Ji-Wen Yang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China. Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Ying-Chun OuYang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China.
Qing-Yuan Sun
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China.
Da-Yuan Chen*
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China.
*
1 All correspondence to: Da-Yuan Chen, State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, #25 Bei Si Huan Xi Road, Haidian, Beijing 100080, PR China. Tel: +86 10 64807052. Fax: +86 10 64807099. e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Early studies have shown that some mouse cumulus–oocyte complexes (COCs) stored at room temperature for 24 h still retained full developmental potential. In this study, we stored denuded mouse oocytes (DOs) at room temperature (25 °C) for 24 h and activated these oocytes with 10 mM SrCl2 or fertilized the oocytes by IVF. We found that nearly half of the DOs stored at room temperature for 1 day can be fertilized normally by IVF and that two foster mothers gave birth to seven pups. Embryos from stored oocytes were cultured in CZB medium with or without 1 μg/ml 17β-estradiol (E2). The numbers of embryo that developed to morula/blastocyst stage after parthenogenetic activation and IVF were significantly increased when E2 was added to the culture (p < 0.05). These results suggest that E2 might improve mouse embryo development in vitro. The birth of seven agouti pups and their healthy growth indicated that the storage of DOs at room temperature for 1 day may be a practical procedure for mammalian reproduction.

Keywords

Embryo development17β-EstradiolIVFOocytePup
Type
Research Article
Information
Zygote , Volume 16 , Issue 1 , February 2008 , pp. 21 - 27
Copyright
Copyright © Cambridge University Press 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abbott, A.L., Xu, Z., Kopf, G.S., Ducibella, T. & Schultz, R.M. (1998). In vitro culture retards spontaneous activation of cell cycle progression and cortical granule exocytosis that normally occur in in vivo unfertilized mouse eggs. Biol. Reprod. 59, 1515–21.CrossRefGoogle ScholarPubMed
Beker, A.R.C.L., Colenbrander, B. & Bevers, M.M. (2002). Effect of 17β-estradiol on the in vitro maturation of bovine oocytes. Theriogenology 58, 1663–73.CrossRefGoogle ScholarPubMed
Buccione, R., Schroeder, A.C. & Eppig, J.J. (1990). Interactions between somatic cells and germ cells throughout mammalian oogenesis. Biol. Reprod. 43, 543–7.CrossRefGoogle ScholarPubMed
Cummins, J.M., Wakayama, T. & Yanagimachi, R. (1998). Fate of microinjected spermatid mitochondria in the mouse oocyte and embryo. Zygote 6, 213–22.CrossRefGoogle ScholarPubMed
Downs, S.M., Schroeder, A.C. & Eppig, J.J. (1986). Serum maintains the fertilizability of mouse oocytes matured in vitro by preventing the hardening of the zona pellucida. Gamete Res. 15, 115–22.CrossRefGoogle Scholar
Eppig, J.J. (1982). The relationship between cumulus cell–oocyte coupling, oocyte meiotic maturation and cumulus expansion. Dev. Biol. 89, 268–72.CrossRefGoogle ScholarPubMed
Eppig, J.J. (1991). Intercommunication between mammalian oocytes and companion somatic cells. Bioessays 13, 569–74.CrossRefGoogle ScholarPubMed
Gordo, A.C., Rodrigues, P., Kurokawa, M., Jellerette, T., Exley, G.E., Warner, C. & Fissore, R. (2002). Intracellular calcium oscillations signal apoptosis rather than activation in in vitro aged mouse eggs. Biol. Reprod. 66, 1828–37.CrossRefGoogle ScholarPubMed
Guler, A., Poulin, N., Mermillod, P., Terqui, M. & Cognie, Y. (2000). Effect of growth factors, EGF and IGF-1 and estradiol on in vitro maturation of sheep oocytes. Theriogenology 54, 209–18.CrossRefGoogle ScholarPubMed
Juetten, J.B.B. (1983). Effects of egg aging on in vitro fertilization and first cleavage division in the hamster. Gamete Res. 8, 219–30.CrossRefGoogle Scholar
Kikuchi, K., Izaike, Y., Noguchi, J., Furukawa, T., Daen, F.P., Naito, K. & Toyoda, Y. (1995). Decrease of histone H1 kinase activity in relation to parthenogenetic activation of pig follicular oocytes matured and aged in vitro. J. Reprod. Fertil. 105, 325–30.CrossRefGoogle ScholarPubMed
Kikuchi, K., Naito, K., Noguchi, J., Shimada, A., Kaneko, H., Yamashita, M., Aoki, F., Tojo, H. & Toyoda, Y. (2000). Maturation/M-phase promoting factor: a regulator of aging in porcine oocytes. Biol. Reprod. 63, 715–22.CrossRefGoogle ScholarPubMed
Kim, N.H., Moon, S.J., Prather, R.S. & Day, B.N. (1996). Cytoskeletal alteration in aged porcine oocytes and parthenogenesis. Mol. Reprod. Dev. 43, 513–8.3.0.CO;2-#>CrossRefGoogle ScholarPubMed
Kishikawa, H., Wakayama, T. & Yanagimachi, R. (1999). Comparison of oocyte-activating agents for mouse cloning. Cloning 1, 153–9.CrossRefGoogle ScholarPubMed
Longo, F. (1980). Aging of mouse eggs in vivo and in vitro. Gamete Res. 3, 379–93.CrossRefGoogle Scholar
Longo, F.J. (1974). Ultrastructural changes in rabbit eggs aged in vivo. Biol. Reprod. 11, 22–39.CrossRefGoogle ScholarPubMed
Miao, Y.L., Liu, X.Y., Qiao, T.W., Miao, D.Q., Luo, M.J. & Tan, J.H. (2005). Cumulus cells accelerate aging of mouse oocytes. Biol. Reprod. 73, 1025–31.CrossRefGoogle ScholarPubMed
Nakagata, N. (2000). Cryopreservation of mouse spermatozoa. Mamm. Genome 11, 572–6.CrossRefGoogle ScholarPubMed
Polge, C. (1952). Fertilizing capacity of bull spermatozoa after freezing at 79 degrees C. Nature 169 (4302), 626–7.CrossRefGoogle ScholarPubMed
Suzuki, H., Shingai, S., Togashi, M., Miyai, T. & Okamoto, M. (1987). In vitro fertilization and the development of eggs following transfer in genetically diabetic obese (db/db) mouse. Jpn. J. Anim. Reprod. 33, 96–100.CrossRefGoogle Scholar
Suzuki, H., Shingai, S., Togashi, M., Miyai, T., Motohide, Y. & Okamoto, M. (1988). The development of newborn C57BL/KsJ-dbm mice produced from eggs fertilized in vitro. Exp. Anim. 37, 245–9.CrossRefGoogle ScholarPubMed
Suzuki, H., Togashi, M., Moriguchi, Y. & Adachi, J. (1994). Relationship between age-related decline in fertility and in vitro fertilization rate in IVCS mice. J. Reprod. Dev. 40, 107–16.CrossRefGoogle Scholar
Suzuki, H., Yorozu, K., Watanabe, T., Nakura, M. & Adachi, J. (1996). Rederivation of mice by means of in vitro fertilization and embryo transfer. Exp. Anim. 45, 33–8.CrossRefGoogle ScholarPubMed
Takeda, K., Tasai, M., Iwamoto, M., Onishi, A., Tagami, T., Nirasawa, K., Hanada, H. & Pinkert, C.A. (2005). Microinjection of cytoplasm or mitochondria derived from somatic cells affects parthenogenetic development of murine oocytes. Biol. Reprod. 72, 1397–404.CrossRefGoogle ScholarPubMed
Tan, J. (1985). An ultrastructural study on the pig oocyte during its aging after ovulation. Acta Vet Zootech Sinica 16, 1–4.Google Scholar
Tan, J.H. (1988). Studies on the Goat Egg. Harbin, People's Republic of China: Northeast Agricultural College; Ph.D. Thesis.Google Scholar
Tanghe, S., Van Soom, A., Nauwynck, H., Coryn, M., de Kruif, A. (2002). Minireview: Functions of the cumulus oophorus during oocyte maturation, ovulation and fertilization. Mol. Reprod. Dev. 61, 414–24.CrossRefGoogle ScholarPubMed
Tarin, J.J., Perez-Albala, S., Aguilar, A., Minarro, J., Hermenegildo, C. & Cano, A. (1999). Long-term effects of postovulatory aging of mouse oocytes on offspring: a two-generational study. Biol. Reprod. 61, 1347–55.CrossRefGoogle ScholarPubMed
Tarin, J.J., Perez-Albala, S., Perez-Hoyos, S. & Cano, A. (2002). Postovulatory aging of oocytes decreases reproductive fitness and longevity of offspring. Biol. Reprod. 66, 495–9.CrossRefGoogle ScholarPubMed
Tesarik, J. & Mendoza, C. (1995). Nongenomic effects of 17beta-estradiol on maturing human oocytes: relationship to oocytes developmental potential. J. Clin. Endocrinol. Metab. 80, 1438–43.Google Scholar
Wakayama, S., Thuan, N.V., Kishigami, S., Ohta, H., Mizutani, E., Hikichi, T., Miyake, M. & Wakayama, T. (2004). Production of offspring from one-day-old oocytes stored at room temperature. J. Reprod. Dev. 50, 627–37.CrossRefGoogle ScholarPubMed
Wakayama, T. & Yanagimachi, R. (1998). Development of normal mice from oocytes injected with freeze-dried spermatozoa. Nat. Biotechnol. 16, 639–41.CrossRefGoogle ScholarPubMed
Wakayama, T., Whittingham, D.G. & Yanagimachi, R. (1998). Production of normal offspring from mouse oocytes injected with spermatozoa cryopreserved with or without cryoprotection. J. Reprod. Fertil. 112, 11–7.CrossRefGoogle ScholarPubMed
Watson, P.F. (1990). Artificial insemination and the preservation of semen. Marshail's Physiology of Reproduction (ed. , E.E Lamming.). pp. 746–869. London: Churchill Livingstone.Google Scholar
Webb, M., Howlett, S.K. & Maro, B. (1986). Parthenogenesis and cytoskeletal organization in ageing mouse eggs. J. Embryol. Exp. Morphol. 95, 131–45.Google ScholarPubMed
Whittingham, D.G. & Siracusa, G. (1978). The involvement of calcium in the activation of mammalian oocytes. Exp. Cell Res. 113, 311–7.CrossRefGoogle ScholarPubMed
Wu, B., Ignotz, G., Currie, W.B. & Yang, X. (1997). Dynamics of maturation-promoting factor and its constituent proteins during in vitro maturation of bovine oocytes. Biol. Reprod. 56, 253–9.CrossRefGoogle ScholarPubMed
Xu, Z., Abbott, A, Kopf, G.S., Schultz, R.M. & Ducibella, T. (1997). Spontaneous activation of ovulated mouse eggs: time-dependent effects on M-phase exit, cortical granule exocytosis, maternal messenger ribonucleic acid recruitment and inositol 1,4,5-trisphosphate sensitivity. Biol. Reprod. 57, 743–50.CrossRefGoogle ScholarPubMed
Yanagimachi, R. & Chang, M.C. (1961). Fertilizable life of golden hamster ova and their morphological changes at the time of losing fertilizability. J. Exp. Zool. 148, 185–203.CrossRefGoogle ScholarPubMed
Yokoyama, M., Katsuki, M. & Nomura, T. (1995). The creation of mouse models from human diseases associated with reproductive disturbance by in vitro fertilization and embryo transfer. Exp. Anim. 44, 139–43.CrossRefGoogle ScholarPubMed
Yu, J.N., Ma, S.F., Miao, D.Q., Tan, X.W., Liu, X.Y., Lu, J.H. & Tan, J.H. (2006). Effects of cell cycle status on the efficiency of liposome-mediated gene transfection in mouse fetal fibroblasts. J. Reprod. Dev. 52, 373–82.CrossRefGoogle ScholarPubMed
Zhang, L.S. (2004). Studies on Mammalian Somatic Cell Nuclear Transfer. Beijing, People's Republic of China: Graduated University of Chinese Academy of Sciences; Ph.D. Thesis.Google Scholar