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Frozen–thawed ampullary cell monolayer improves bovine embryo in vitro development and quality

Published online by Cambridge University Press:  13 August 2019

Anise Asaadi ,
Mojtaba Kafi Open the ORCID record for Mojtaba Kafi [Opens in a new window] ,
Hadi Atashi ,
Mehdi Azari  and
Miel Hostens
Anise Asaadi
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Mojtaba Kafi*
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Hadi Atashi
Affiliation:
Department of Animal Science, School of Agriculture, Shiraz University, Shiraz, Iran
Mehdi Azari
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Miel Hostens
Affiliation:
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
*
Address for correspondence: Mojtaba Kafi. Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. E-mail: [email protected]
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Summary

The aim of this study was to evaluate the effects of different timing for frozen–thawed bovine ampullary epithelial cell (BAEC) and bovine oviductal epithelial cell (BOEC) co-culture on the development and quality of bovine embryos produced in vitro. Embryo development was assessed by day 8 blastocyst yield, whereas embryo quality was determined using blastocyst differential cell count, cryotolerance and the expression of selected genes related to embryo quality. The results showed that the presence of BAECs during the last 6 h of in vitro maturation (IVM) increased blastocyst yield and survival of the vitrified-warmed blastocysts. In addition, embryos produced in the presence of BAECs during the last 6 h of IVM or in the presence of BOECs during the first 4 days of in vitro culture (IVC) showed a greater number of trophectoderm cells and a greater inner cell mass. In terms of gene expression, IFN-T was downregulated and PLAC8, AQP3 and ATP1A1 were upregulated in the presence of the BAECs during the last 6 h of the IVM and/or in the presence of BOECs during the first 4 days of IVC. In conclusion, co-culturing bovine oocytes with a frozen–thawed ampullary cell monolayer during the last 6 h of maturation increased blastocyst yield and quality.

Keywords

AmpullaEmbryo productionGene expressionOocyte maturation
Type
Research Article
Information
Zygote , Volume 27 , Issue 5 , October 2019 , pp. 337 - 346
Copyright
© Cambridge University Press 2019 

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