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Folic acid supplementation during oocytes maturation influences in vitro production and gene expression of bovine embryos

Published online by Cambridge University Press:  09 March 2021

Carolina Gennari Verruma Open the ORCID record for Carolina Gennari Verruma [Opens in a new window] ,
Matheus Credendio Eiras ,
Artur Fernandes ,
Reginaldo Aparecido Vila ,
Cristiana Libardi Miranda Furtado ,
Ester Silveira Ramos  and
Raysildo Barbosa Lôbo
Carolina Gennari Verruma*
Affiliation:
Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil
Matheus Credendio Eiras
Affiliation:
Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil
Artur Fernandes
Affiliation:
Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil
Reginaldo Aparecido Vila
Affiliation:
Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil
Cristiana Libardi Miranda Furtado
Affiliation:
Drug Research and Development Center, Postgraduate Programme in Medical and Surgical Sciences, Federal University of Ceara, CE, Brazil
Ester Silveira Ramos
Affiliation:
Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil
Raysildo Barbosa Lôbo
Affiliation:
Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil
*
Author for correspondence: Carolina Gennari Verruma. Genetics Department, Medical School of Ribeirão Preto, Bandeirantes Avenue, 3900, 14049-900, São Paulo University, SP, Brazil. Tel: +55 16 3315 4909. E-mail: [email protected]
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Summary

Embryos that are produced in vitro frequently present epigenetic modifications. However, maternal supplementation with folic acid (FA) may improve oocyte maturation and embryo development, preventing epigenetic errors in the offspring. We sought to evaluate the influence of FA supplementation during in vitro maturation of grade I (GI) and grade III (GIII) bovine oocytes on embryo production rate and the expression of IGF2 and KCNQ1OT1 genes. The oocytes were matured in vitro with different concentrations of FA (0, 10, 30 and 100 μM), followed by in vitro fertilization and embryo culture. On the seventh day (D7) of culture, embryo production was evaluated and gene expression was measured using real-time qPCR. Supplementation with 10 μM of FA did not affect embryo production for GI and GIII oocytes. Moderate supplementation (30 μM) seemed to be a positive influence, increasing embryo production for GIII (P = 0.012), while the highest dose (100 μM) reduced embryo production (P = 0.010) for GI, and IGF2 expression was not detected. In GIII, only embryos whose oocyte maturation was not supplemented with FA demonstrated detected IGF2 expression. The lowest concentration of FA (10 μM) reduced KCNQ1OT1 expression (P = 0.05) on embryos from GIII oocytes. Different FA concentrations induced different effects on bovine embryo production and gene expression that was related to oocyte quality. Despite the epigenetic effects of FA, supplementation seems to be a promising factor to improve bovine embryo production if used carefully, as concentration is an important factor, especially in oocytes with impaired quality.

Keywords

Embryo early developmentEpigeneticsImprinted genesMethyl donorsOocyte supplementation
Type
Research Article
Information
Zygote , Volume 29 , Issue 5 , October 2021 , pp. 342 - 349
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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