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Fertilizability and chromosomal integrity of frozen-thawed Bryde's whale (Balaenoptera edeni) spermatozoa intracytoplasmically injected into mouse oocytes

Published online by Cambridge University Press:  01 February 2007

H. Watanabe ,
H. Tateno ,
H. Kusakabe ,
T. Matsuoka ,
Y. Kamiguchi ,
Y. Fujise ,
H. Ishikawa ,
S. Ohsumi  and
Y. Fukui
H. Watanabe
Affiliation:
Department of Animal Production Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080–8555, Japan.
H. Tateno
Affiliation:
Department of Biological Sciences, Asahikawa Medical College, Asahikawa 078–8510, Japan.
H. Kusakabe
Affiliation:
Department of Biological Sciences, Asahikawa Medical College, Asahikawa 078–8510, Japan.
T. Matsuoka
Affiliation:
Department of Animal Production Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080–8555, Japan.
Y. Kamiguchi
Affiliation:
Department of Biological Sciences, Asahikawa Medical College, Asahikawa 078–8510, Japan.
Y. Fujise
Affiliation:
The Institute of Cetacean Research, Tokyo 104–0055, Japan.
H. Ishikawa
Affiliation:
The Institute of Cetacean Research, Tokyo 104–0055, Japan.
S. Ohsumi
Affiliation:
The Institute of Cetacean Research, Tokyo 104–0055, Japan.
Y. Fukui*
Affiliation:
Department of Animal Production Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080–8555, Japan.
*
All correspondence to: Yutaka Fukui, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080–8555, Japan. Tel: +81–155–49–5415. Fax: +81–155–49–5593. e-mail: [email protected]
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Summary

Prior to attempting the in vitro production of embryos in the Bryde's whale (Balaenoputera edeni), we investigated whether spermatozoa can retain the capacity for oocyte activation and pronucleus formation as well as chromosomal integrity under cryopreservation by using intracytoplasmic sperm injection (ICSI) into mouse oocytes. Regardless of motility and viability, whale spermatozoa efficiently led to the activation of mouse oocytes (90.3–97.4%), and sperm nuclei successfully transformed into male pronucleus within activated ooplasm (87.2–93.6%). Chromosome analysis at the first cleavage metaphase (M) of the hybrid zygotes revealed that a majority (95.2%) of motile spermatozoa had the normal chromosome complement, while the percentage of chromosomal normality was significantly reduced to 63.5% in immotile spermatozoa and 50.0% in dead spermatozoa due to the increase in structural chromosome aberrations. This is the first report showing that motile Bryde's whale spermatozoa are competent to support embryonic development.

Keywords

Assisted reproductive technologyBryde's whaleChromosomeFertilizationIntracytoplasmic sperm injection
Type
Research Article
Information
Zygote , Volume 15 , Issue 1 , February 2007 , pp. 9 - 14
Copyright
Copyright © Cambridge University Press 2007

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References

Amemiya, K., Iwanami, Y., Kobayashi, T., Terao, T., Fukui, Y., Ishikawa, H., Ohsumi, S., Hirabayashi, M. & Hochi, S. (2004). Acquirement of oocyte-activating factor in Antarctic minke whale (Balaenoptera bonaerensis) spermatogenic cells, assessed by meiosis resumption of microinseminated mouse oocytes. J. Mamm. Ova. Res. 21, 149–56.CrossRefGoogle Scholar
Asada, M., Horii, M., Mogoe, T., Fukui, Y., Ishikawa, H. & Ohsumi, S. (2000). In vitro maturation and ultrastructural observation of cryopreserved minke whale (Balaenoptera acutorostrata) follicular oocytes. Biol. Reprod. 62, 253–9.CrossRefGoogle ScholarPubMed
Asada, M., Tetsuka, M., Ishikawa, H., Ohsumi, S. & Fukui, Y. (2001a). Improvement on in vitro maturation, fertilization and development of minke whale (Balaenoptera acutorostrata) oocytes. Theriogenology 56, 521–33.CrossRefGoogle ScholarPubMed
Asada, M., Wei, H., Nagayama, R., Tetsuka, M., Ishikawa, H., Ohsumi, S. & Fukui, Y. (2001b). An attempt at intracytoplasmic sperm injection of frozen-thawed minke whale (Balaenoptera bonaerensis) oocytes. Zygote 9, 299–307.CrossRefGoogle ScholarPubMed
Chatot, C.L., Ziomek, C.A., Bavister, B.D., Lewis, J.L. & Torres, I. (1989). An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J. Reprod. Fertil. 86, 679–88.CrossRefGoogle ScholarPubMed
Fedorova, I.D., Kuznetsova, T.V., Baranov, V.S., Rybouchkin, A.V., Van Der Elst, J. & Dhont, M. (2005). Cytogenetic analysis of human spermatozoa using intracytoplasmic sperm injection into mouse oocytes. Genetika 41, 396–404.Google ScholarPubMed
Fukui, Y., Mogoe, T., Ishikawa, H. & Ohsumi, S. (1997a). Factors affecting in vitro maturation of minke whale (Balaenoptera acutorostrata) follicular oocytes. Biol. Reprod. 56, 523–8.CrossRefGoogle ScholarPubMed
Fukui, Y., Mogoe, T., Ishikawa, H. & Ohsumi, S. (1997b). In vitro fertilization of in vitro matured minke whale (Balaenoptera acutorostrata) follicular oocytes. Mar. Mamm. Sci. 13, 395–404.CrossRefGoogle Scholar
Iwayama, H., Hochi, S., Kato, M., Hirabayashi, M., Kuwayama, M., Ishikawa, H., Ohsumi, S. & Fukui, Y. (2004). Effects of cryodevice type and donors’ sexual maturity on vitrification of minke whale (Balaenoptera bonaerensis) oocytes at germinal vesicle stage. Zygote 12, 333–8.CrossRefGoogle ScholarPubMed
Kimura, Y. & Yanagimachi, R. (1995). Intracytoplasmic sperm injection in the mouse. Biol. Reprod. 52, 709–20.CrossRefGoogle ScholarPubMed
Kimura, Y., Yanagimachi, R., Kuretake, S., Bortkiewicz, H., Perry, A.C. & Yanagimachi, H. (1998). Analysis of mouse oocyte activation suggests the involvement of sperm perinuclear material. Biol. Reprod. 58, 1407–15.CrossRefGoogle ScholarPubMed
Lee, J.D., Kamiguchi, Y. & Yanagimachi, R. (1996). Analysis of chromosome constitution of human spermatozoa with normal and aberrant head morphologies after injection into mouse oocytes. Hum. Reprod. 11, 1942–6.CrossRefGoogle ScholarPubMed
McLay, D.W. & Clarke, H.J. (2003). Remodelling the paternal chromatin at fertilization in mammals. Reproduction 125, 625–33.CrossRefGoogle ScholarPubMed
Mikamo, K. & Kamiguchi, Y. (1983). A new assessment system for chromosomal mutagenicity using oocytes and early zygotes of the Chinese hamster. In Radiation-induced chromosome damage in man, (eds Ishihara, T. & Sasaki, M.S.), pp. 411–32. New York: Alan R. Liss.Google Scholar
Mogoe, T., Fukui, Y., Ishikawa, H. & Ohsumi, S. (1998). Morphological observations of frozen-thawed spermatozoa of southern minke whales (Balaenoptera acutorostrata). J. Reprod. Dev. 44, 95–100.CrossRefGoogle Scholar
Rybouchkin, A., Benijts, J., De Sutter, P. & Dhont, M. (1997). Disintegration of chromosomes in dead sperm cells as revealed by injection into mouse oocytes. Hum. Reprod. 12, 1693–8.CrossRefGoogle ScholarPubMed
Rybouchkin, A., Dozortsev, D., De Sutter, P., Qian, C. & Dhont, M. (1995). Intracytoplasmic injection of human spermatozoa into mouse oocytes: a useful model to investigate the oocyte-activating capacity and the karyotype of human spermatozoa. Hum. Reprod. 10, 1130–5.CrossRefGoogle ScholarPubMed
Rybouchkin, A., Dozortsev, D., Pelinck, M.J., De Sutter, P. & Dhont, M. (1996a). Analysis of the oocyte activating capacity and chromosomal complement of round-headed human spermatozoa by their injection into mouse oocytes. Hum. Reprod. 11, 2170–5.CrossRefGoogle ScholarPubMed
Rybouchkin, A.V., De Sutter, P. & Dhont, M. (1996b). Unprotected freezing of human spermatozoa exerts a detrimental effect on their oocyte activating capacity and chromosome integrity. Zygote 4, 263–8.CrossRefGoogle ScholarPubMed
Tateno, H. & Kamiguchi, Y. (1999a). Dithiothreitol induces sperm nuclear decondensation and protects against chromosome damage during male pronuclear formation in hybrid zygotes between Chinese hamster spermatozoa and Syrian hamster oocytes. Zygote 7, 321–7.CrossRefGoogle ScholarPubMed
Tateno, H. & Kamiguchi, Y. (1999b). Improvement of male pronuclear formation during cross-fertilization between Chinese hamster spermatozoa and Syrian hamster oocytes by nocodazole, and chromosome analysis of hybrid zygotes. Mol. Reprod. Dev. 52, 117–24.3.0.CO;2-S>CrossRefGoogle Scholar
Tateno, H. & Kamiguchi, Y. (2005). How long do parthenogenetically activated mouse oocytes maintain the ability to accept sperm nuclei as a genetic partner? J. Assist. Reprod. Genet. 22, 89–93.CrossRefGoogle Scholar
Tateno, H., Kimura, Y. & Yanagimachi, R. (2000). Sonication per se is not as deleterious to sperm chromosomes as previously inferred. Biol. Reprod. 63, 341–6.CrossRefGoogle Scholar
Toyoda, Y., Yokoyama, M. & Hoshi, T. (1971). Studies on the fertilization of mouse eggs in vitro; I. In vitro fertilization of mouse eggs by fresh epididymal semen. Jpn J. Anim. Reprod. 16, 147–51.Google Scholar
Watanabe, H., Mogoe, T., Asada, M., Hayashi, K., Fujise, Y., Ishikawa, H., Ohsumi, S., Miyamoto, A. & Fukui, Y. (2004). Relationship between serum sex hormone concentrations and histology of seminiferous tubules of captured baleen whales in the Western North Pacific during the feeding season. J. Reprod. Dev. 50, 419–27.CrossRefGoogle ScholarPubMed
Watanabe, S. (2003). A detailed cytogenetic analysis of large numbers of fresh and frozen-thawed human sperm after ICSI into mouse oocytes. Hum. Reprod. 18, 1150–7.CrossRefGoogle ScholarPubMed
Watanabe, S. (2004). Frequent structural chromosome aberrations in immotile human sperm exposed to culture media. Hum. Reprod. 19, 940–7.CrossRefGoogle ScholarPubMed
Wei, H. & Fukui, Y. (2000). Fertilisability of ovine, bovine or minke whale (Balaenoptera acutorostrata) spermatozoa intracytoplasmically injected into bovine oocytes. Zygote 8, 267–74.CrossRefGoogle ScholarPubMed