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Expression of genes associated with fertility in the uterus and oviduct of heifers challenged with lipopolysaccharide

Published online by Cambridge University Press:  12 January 2022

Giuliana A. Ferronato ,
Joao A. Alvarado-Rincón Open the ORCID record for Joao A. Alvarado-Rincón [Opens in a new window] ,
Andressa S. Maffi ,
Antônio A. Barbosa ,
Bernardo G. Gasperin ,
Augusto Schneider ,
Rafael G. Mondadori Open the ORCID record for Rafael G. Mondadori [Opens in a new window] ,
Cássio C. Brauner  and
Marcio N. Corrêa
Giuliana A. Ferronato
Affiliation:
Facultad de Ciencias Agropecuarias, Universidad de la Salle, Campus Utopia, Yopal - Casanare, 850008, Colombia
Joao A. Alvarado-Rincón
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Andressa S. Maffi
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Antônio A. Barbosa
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Bernardo G. Gasperin
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Augusto Schneider
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Rafael G. Mondadori
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Cássio C. Brauner
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Marcio N. Corrêa*
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
*
Author for correspondence: Marcio Nunes Corrêa. Federal University of Pelotas, Pelotas, RS96160–000, Brazil. E-mail: [email protected]
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Summary

Lipopolysaccharide (LPS) endotoxemia has been negatively associated with fertility. This study aimed to investigate the effect of LPS-induced inflammation on gene expression associated with bovine fertility in the uterus and oviduct. Sixteen healthy heifers were divided into two groups. The LPS group (n = 8) received two intravenous (i.v.) injections of 0.5 µg/kg of body weight of LPS with a 24-h interval, and the control group (n = 8) received two i.v. injections of saline solution with the same interval of time. All the animals had the follicular wave synchronized. Three days after the second injection of LPS, all animals were slaughtered and uterine and oviduct samples were collected. Gene expression associated with inflammatory response, thermal and oxidative stresses, oviduct environment quality, and uterine environment quality was evaluated. Body temperature and leucogram demonstrated that LPS induced an acute systemic inflammatory response. In the uterus, the expression of PTGS2 and NANOG genes was downregulated by the LPS challenge. However, no change in expression was observed in the other evaluated genes in the uterus, nor those evaluated in the oviduct. In conclusion, the inflammatory process triggered by LPS did not persist in the uterus and oviduct 3 days after challenge with LPS. Nonetheless, reduction in PTGS2 and NANOG expression in the uterus suggested that, indirectly, LPS may have a prolonged effect, which may affect corpus luteum and endometrial functions.

Keywords

CattleEndotoxemiaLPSInflammationReproduction
Type
Short Communication
Information
Zygote , Volume 30 , Issue 4 , August 2022 , pp. 584 - 587
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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References

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