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Embryo production by parthenogenetic activation and fertilization of in vitro matured oocytes from Cebus apella

Published online by Cambridge University Press:  10 January 2012

Julianne S. Lima ,
Danuza L. Leão ,
Rafael V. Sampaio ,
Adriel B. Brito ,
Regiane R. Santos ,
Moysés S. Miranda ,
Otávio M. Ohashi  and
Sheyla F. S. Domingues
Julianne S. Lima*
Affiliation:
Rua Augusto Corrêa, Campus Básico, CEP 66075–110, Belém, Pará, Brazil. Laboratory of Wild Animal Biology and Medicine, Universidade Federal do Pará, Brazil. Animal Science Post-graduation Program, Universidade Federal do Pará, Brazil.
Danuza L. Leão
Affiliation:
Laboratory of Wild Animal Biology and Medicine, Universidade Federal do Pará, Brazil.
Rafael V. Sampaio
Affiliation:
Laboratory of In Vitro Fertilization, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil.
Adriel B. Brito
Affiliation:
Laboratory of Wild Animal Biology and Medicine, Universidade Federal do Pará, Brazil. Animal Science Post-graduation Program, Universidade Federal do Pará, Brazil.
Regiane R. Santos
Affiliation:
Laboratory of Wild Animal Biology and Medicine, Universidade Federal do Pará, Brazil. Animal Science Post-graduation Program, Universidade Federal do Pará, Brazil. Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Moysés S. Miranda
Affiliation:
Laboratory of In Vitro Fertilization, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil.
Otávio M. Ohashi
Affiliation:
Animal Science Post-graduation Program, Universidade Federal do Pará, Brazil. Laboratory of In Vitro Fertilization, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil.
Sheyla F. S. Domingues
Affiliation:
Laboratory of Wild Animal Biology and Medicine, Universidade Federal do Pará, Brazil. Animal Science Post-graduation Program, Universidade Federal do Pará, Brazil.
*
All correspondence to: Julianne S. Lima. Rua Augusto Corrêa, Campus Básico, CEP 66075–110, Belém, Pará, Brazil. Tel:/Fax: +55 81 32018011. e-mail: [email protected]
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Summary

The efficiency of in vitro fertilization (IVF) depends on the viability of spermatozoa. For capuchin monkeys (Cebus apella), in vitro capacitation of spermatozoa is challenging because of their unique seminal coagulum. Motile spermatozoa can be obtained after liquefaction of the semen coagulum in coconut water-based solution. The objective of the present study was to establish an optimal in vitro maturation (IVM) protocol for capuchin monkeys and to observe the effect of follicle stimulating hormone (FSH) and luteinising hormone (LH) on IVF and parthenogenetic activation (PA) of oocytes collected from unstimulated females. We assessed spermatozoa quality after recovery from seminal coagulum using the solution ACP-118® as an extender. Oocytes were matured in vitro for 36 or 40 h and subjected to IVF or PA by applying ionomycin combined either with 6-dimethylaminopurine (6-DMAP) or roscovitine. In total, 87% of oocytes reached metaphase II (MII) after 40 IVM and 4-cell embryo production was obtained after IVF and parthenogenesis using ionomycin/6-DMAP. ACP-118® was used successfully to harvest viable spermatozoa from semen coagulum and in the preservation of spermatozoa, which were able to fertilize oocytes in vitro.

Keywords

ACP-118®Capuchin monkeysFertilizationMaturationParthenogenesis
Type
Research Article
Information
Zygote , Volume 21 , Issue 2 , May 2013 , pp. 162 - 166
Copyright
Copyright © Cambridge University Press 2012

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