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The effect of bovine embryo culture without proteins supplements until day 4 on transcription level of hyaluronan synthases, receptors and mtDNA content

Published online by Cambridge University Press:  11 December 2009

A.T. Palasz ,
P. Beltrán Breña ,
J. De la Fuente  and
A. Gutiérrez-Adán
A.T. Palasz*
Affiliation:
Ministry of Science and Innovation, Department of Animal Reproduction, INIA, Madrid, Spain
P. Beltrán Breña
Affiliation:
Ministry of Science and Innovation, Department of Animal Reproduction, INIA, Madrid, Spain
J. De la Fuente
Affiliation:
Ministry of Science and Innovation, Department of Animal Reproduction, INIA, Madrid, Spain
A. Gutiérrez-Adán
Affiliation:
Ministry of Science and Innovation, Department of Animal Reproduction, INIA, Madrid, Spain
*
1All correspondence to: A.T. Palasz. Dpto. de Reproducción Animal y Conservación de Recursos Zoogenéticos INIA, Ctra de la Coruña Km 5.9, Madrid 28040, Spain. Fax: +34 91 347 4014. e-mail; [email protected]
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Summary

The effect of bovine embryo culture on a flat surface, (without a surface-active compound) on the level of mRNA expression of hyaluronan (HA) synthases (Has1, Has2 and Has3), Ha receptors RHAMM and C44 receptors was evaluated by mitochondrial DNA concentration and in vitro development. Cultures were evaluated up to 96 h post-insemination (hpi) using SOFaa medium. Of the three Has isoforms, Has2 expression only increased in the bovine serum albumin (BSA)-only supplemented groups regardless of time of BSA addition. Expression of RHAMM receptors was highly dependent on the addition of HA, irrespective of the presence of BSA in the medium. In contrast, expression of the CD44 receptor gene was not affected by any treatment. The cleavage rates and number of embryos that developed to ≤8-cell stage by day 4 were not affected by lack of BSA in the medium, but increased numbers of blastocysts developed in medium supplemented with BSA from days 1 or 4 with or without HA than in medium that had HA only. Addition of both HA and BSA at day 4 increased mtDNA copy numbers at the blastocyst stage. Data suggest that the addition of BSA and/or HA at 96 hpi increased expression of RHAMM and Has2 genes, but not CD44, Has1 or Has3 genes. Higher expression levels of Has2 than Has1 and the three isoforms indicate that high- rather than low-molecular-weight HA should be used for preimplantation bovine embryo culture.

Keywords

CultureEmbryoGene expressionHyaluronanMitochondriaSynthases
Type
Research Article
Information
Zygote , Volume 18 , Issue 2 , May 2010 , pp. 121 - 129
Copyright
Copyright © Cambridge University Press 2009

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