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Ectopic autologous transplantation of ovarian tissue as a feasible technique to assess ovarian morphophysiology

Published online by Cambridge University Press:  29 September 2021

Bárbara Rodrigues Nascimento ,
Guilherme Antonio de Gouvêa Lopes ,
Danielle Storino de Freitas  and
Paulo Henrique Almeida Campos-Junior Open the ORCID record for Paulo Henrique Almeida Campos-Junior [Opens in a new window]
Bárbara Rodrigues Nascimento
Affiliation:
Laboratório de Pesquisa em Reprodução e Desenvolvimento, Universidade Federal de São João del Rei. Praça Dom Helvécio, 74 – Dom Bosco, São João del Rei, MG, 36301-160, Brazil
Guilherme Antonio de Gouvêa Lopes
Affiliation:
Laboratório de Pesquisa em Reprodução e Desenvolvimento, Universidade Federal de São João del Rei. Praça Dom Helvécio, 74 – Dom Bosco, São João del Rei, MG, 36301-160, Brazil
Danielle Storino de Freitas
Affiliation:
Laboratório de Pesquisa em Reprodução e Desenvolvimento, Universidade Federal de São João del Rei. Praça Dom Helvécio, 74 – Dom Bosco, São João del Rei, MG, 36301-160, Brazil
Paulo Henrique Almeida Campos-Junior*
Affiliation:
Laboratório de Pesquisa em Reprodução e Desenvolvimento, Universidade Federal de São João del Rei. Praça Dom Helvécio, 74 – Dom Bosco, São João del Rei, MG, 36301-160, Brazil
*
Author for correspondence: Paulo Henrique Almeida Campos-Junior. Universidade Federal de São João del Rei. Praça Dom Helvécio, 74 – Dom Bosco, São João del Rei, MG, 36301-160, Brazil. E-mail: [email protected]
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Summary

The cryopreservation of murine ovarian tissue and its transplantation can be a promising technique for the preservation of fertility and an alternative for the future reconstitution of scientific valuable strains of mice. Accordingly, the aim of this study was to describe the entire surgical procedure for ovariectomy and dorsal subcutaneous autotransplantation in mice, and also some data about the efficiency of this procedure. Female C57Bl/6J mice (n = 18) were anaesthetised and bilaterally ovariectomized. After surgery, ovaries were autotransplanted in small subcutaneous pouches in the dorsal region of the forelimbs. The animals were inspected daily and, 23 days after transplantation, euthanasia and recovery of ovarian tissues were performed. Postoperative recovery, oestrous cyclicity, and folliculogenesis progression were evaluated. At 23 days after transplantation, the recovery of the ovaries was feasible, all classes (primordial to antral) of follicles were observed. Additionally, satisfactory efficiency rates were obtained, with 100% of anaesthesia survival rate, survival, graft recovery, folliculogenesis progression and oestrous cyclicity. In general, this short article describes ovarian ectopic autologous transplantation as an effective technique for maintaining rodent oogenesis and endocrine ovarian function. Even more broadly, we can still assume that the application of this technique may reduce the number of breeding matrices and experimental animals in the near future.

Keywords

AutotransplantationFertilityGermplasmOvaryRodent
Type
Short Communication
Information
Zygote , Volume 30 , Issue 3 , June 2022 , pp. 416 - 418
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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