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Early mouse preimplantation development is unaffected by microinjection of metallothionein antibodies

Published online by Cambridge University Press:  26 September 2008

Elena Ibánez ,
Francesca Vidal  and
Juan Hidalgo
Elena Ibánez
Affiliation:
Departament de Biologia Cel-lular i Fisiologia, Universitat Autònoma de Barcelona, Barcelona, Spain
Francesca Vidal
Affiliation:
Departament de Biologia Cel-lular i Fisiologia, Universitat Autònoma de Barcelona, Barcelona, Spain
Juan Hidalgo*
Affiliation:
Departament de Biologia Cel-lular i Fisiologia, Universitat Autònoma de Barcelona, Barcelona, Spain
*
Dr Juan Hidalgo, Unitat de Fisiologia Animal, Facultat de Ciències, Departament de Biologia Cel·lular i Fisiologia, Universitat Autònoma de Barcelona, Bellaterra 08193, Barcelona, Spain. Fax: 34–3–581 22 95.
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Summary

Polyclonal antibodies that cross-react with rodent metallothionein I (MT I) and metallothionein II (MT II) were microinjected in 1-cell and 2-cell mouse embryos, into either the cytoplasm or the nucleus. Regardless of the experimental treatment, mouse embryo development in vitro was not affected and most of the embryos cleaved normally until the morula stage. The results suggest that metallothionein is not essential for normal mouse early preimplantational development, in agreement with recent studies in mice with inactivated MT I and MT II genes.

Keywords

Embryo developmentMetallothioneinMicroinjectionMouse
Type
Article
Information
Zygote , Volume 3 , Issue 1 , February 1995 , pp. 81 - 84
Copyright
Copyright © Cambridge University Press 1995

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Footnotes

1United de Biologia Cel-lular, Facultat de Ciències, Departament de Biologia Cel·lular i Fisiologia, Universitat Autònoma de Barcelona, Barcelona, Spain.
2Unitat de Fisiologia Animal, Facultat de Ciències, Departament de Biologia Cel-lular i Fisiologia, Universitat Autònoma de Barcelona, Barcelona, Spain.

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