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Dynamic and aberrant patterns of H3K4me3, H3K9me3, and H3K27me3 during early zygotic genome activation in cloned mouse embryos

Published online by Cambridge University Press:  15 September 2022

Zhihui Liu ,
Jing Cui ,
Weiguo Wang ,
Mingyang Li ,
Zhisong Wang ,
Giorgio Antonio Presicce ,
Xiuchun (Cindy) Tian ,
Liyou An  and
Fuliang Du Open the ORCID record for Fuliang Du [Opens in a new window]
Zhihui Liu
Affiliation:
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing210046, China
Jing Cui
Affiliation:
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing210046, China
Weiguo Wang
Affiliation:
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing210046, China
Mingyang Li
Affiliation:
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing210046, China
Zhisong Wang
Affiliation:
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing210046, China
Giorgio Antonio Presicce
Affiliation:
ARSIAL, Rome00162, Italy
Xiuchun (Cindy) Tian
Affiliation:
Centre for Regenerative Biology/Department of Animal Science, University of Connecticut, Storrs, CT, USA
Liyou An*
Affiliation:
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi830046, China
Fuliang Du*
Affiliation:
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing210046, China
*
Author for correspondence: Fuliang Du’ Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, #1 Wenyuan Rd, Nanjing210046, P R China, Email: [email protected]. Liyou An. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi830046, China. Email: [email protected]
Author for correspondence: Fuliang Du’ Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, #1 Wenyuan Rd, Nanjing210046, P R China, Email: [email protected]. Liyou An. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi830046, China. Email: [email protected]
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Summary

Somatic cell nuclear transfer (NT) is associated with aberrant changes in epigenetic reprogramming that impede the development of embryos, particularly during zygotic genome activation. Here, we characterized epigenetic patterns of H3K4me3, H3K9me3, and H3K27me3 in mouse NT embryos up to the second cell cycle (i.e. four-celled stage) during zygotic genome activation. In vivo fertilized and parthenogenetically activated (PA) embryos served as controls. In fertilized embryos, maternal and paternal pronuclei exhibited asymmetric H3K4me3, H3K9me3, and H3K27me3 modifications, with the paternal pronucleus showing delayed epigenetic modifications. Higher levels of H3K4me3 and H3K9me3 were observed in NT and PA embryos than in fertilized embryos. However, NT embryos exhibited a lower level of H3K27me3 than PA and fertilized embryos from pronuclear stage 3 to the four-celled stage. Our finding that NT embryos exhibited aberrant H3K4me3, H3K9me3, and H3K27me3 modifications in comparison with fertilized embryos during early zygotic genome activation help to unravel the epigenetic mechanisms of methylation changes in early NT reprogramming and provide an insight into the role of histone H3 in the regulation of cell plasticity during natural reproduction and somatic cell NT.

Keywords

Epigenetic modificationMouseNuclear reprogrammingNuclear transfer
Type
Research Article
Information
Zygote , Volume 30 , Issue 6 , December 2022 , pp. 903 - 909
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this study.

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